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DNMT3L (NM_175867) Human Untagged Clone

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SKU
SC110409
DNMT3L (untagged)-Human DNA (cytosine-5-)-methyltransferase 3-like (DNMT3L), transcript variant 2
  $457.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol DNMT3L
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC110409 sequence for NM_175867 edited (data generated by NextGen Sequencing)
ATGGCGGCCATCCCAGCCCTGGACCCAGAGGCCGAGCCCAGCATGGACGTGATTTTGGTG
GGATCCAGTGAGCTCTCAAGCTCCGTTTCACCCGGGACAGGCAGAGATCTTATTGCATAT
GAAGTCAAGGCTAACCAGCGAAATATAGAAGACATCTGCATCTGCTGCGGAAGTCTCCAG
GTTCACACACAGCACCCTCTGTTTGAGGGAGGGATCTGCGCCCCATGTAAGGACAAGTTC
CTGGATGCCCTCTTCCTGTACGACGATGACGGGTACCAATCCTACTGCTCCATCTGCTGC
TCCGGAGAGACGCTGCTCATCTGCGGAAACCCTGATTGCACCCGATGCTACTGCTTCGAG
TGTGTGGATAGCCTGGTCGGCCCCGGGACCTCGGGGAAGGTGCACGCCATGAGCAACTGG
GTGTGCTACCTGTGCCTGCCGTCCTCCCGAAGCGGGCTGCTGCAGCGTCGGAGGAAGTGG
CGCAGCCAGCTCAAGGCCTTCTACGACCGAGAGTCGGAGAATCCCCTTGAGATGTTCGAA
ACCGTGCCTGTGTGGAGGAGACAGCCAGTCCGGGTGCTGTCCCTTTTTGAAGACATCAAG
AAAGAGCTGACGAGTTTGGGCTTTTTGGAAAGTGGTTCTGACCCGGGACAACTGAAGCAT
GTGGTTGATGTCACAGACACAGTGAGGAAGGATGTGGAGGAGTGGGGACCCTTCGATCTT
GTGTACGGCGCCACACCTCCCCTGGGCCACACCTGTGACCGTCCTCCCAGCTGGTACCTG
TTCCAGTTCCACCGGCTCCTGCAGTACGCACGGCCCAAGCCAGGCAGCCCCGGGCCCTTC
TTCTGGATGTTCGTGGACAATCTGGTGCTGAACAAGGAAGACCTGGACGTCGCATCTCGC
TTCCTGGAGATGGAGCCAGTCACCATCCCAGATGTCCACGGCGGATCCTTGCAGAATGCT
GTCCGCGTGTGGAGCAACATCCCAGCCATAAGGAGCAGGCACTGGGCTCTGGTTTCGGAA
GAAGAATTGTCCCTGCTGGCCCAGAACAAGCAGAGCTCGAAGCTCGCGGCCAAGTGGCCC
ACCAAGCTGGTGAAGAACTGCTTTCTCCCCCTAAGAGAATATTTCAAGTATTTTTCAACA
GAACTCACTTCCTCTTTATAA

Clone variation with respect to NM_175867.1
832 a=>g
5' Read Nucleotide Sequence
>OriGene 5' read for NM_175867 unedited
GGAACTTGAGAATTGTAACACCACTTATATAGGCGGCCGCGAATTCGCACGAGGTGGGCC
TGGNACCTTCCACTCATCTTTGCCTGCGCCGTCCCTCGTTCCTGGCCCAGTCCTCCCCGG
GGACCCTGAGCCTGGAAGCCCCGGACCACTGGAACCTTGAACCCACCAGCTGGCTGTACC
CGGAGCCGTGGCAGCAGCCCTCATCCCCATGGCGGCCATCCCAGCCCTGGACCCAGAGGC
CGAGCCCAGCATGGACGTGATTTTGGTGGGATCCAGTGAGCTCTCAAGCTCCGTTTCACC
CGGGACAGGCAGAGATCTTATTGCATATGAAGTCAAGGCTAACCAGCGAAATATAGAAGA
CATCTGCATCTGCTGCGGAAGTCTCCAGGTTCACACACAGCACCCTCTGTTTGAGGGAGG
GATCTGCGCCCCATGTAAGGACAAGTTCCTGGATGCCCTCTTCCTGTACGACGATGACGG
GTACCAATCCTACTGCTCCATCTGCTGCTCCGGAGAGACGCTGCTCATCTGCGGAAACCC
TGATTGCACCCGATGCTACTGCTTCGAGTGTGTGGATAGCCTGGTCGGCCCCGGGACCTC
GGGGAAGGTGCACGCCATGAGCAACTGGGTGTGCTACCTGTGCCTGCCGTCCTCCCGAAG
CGGGCTGCTGCAGCGTCGGAGGAAGTGGCGCAGCCAGCTCAAGGCCTTCTACGACCGAGA
GTCGGAGAATCCCCTTGAGATGTTCGAAACCGTGCCTGTGTGGAGGAGACAGCCAGTCCG
GGTGCTGTNCCTTTTTGAAGACATCAAGAAGACTGACGAGTTTGGGCTTTTTGGAAAAGT
GGTTCTGACCCGGGACACTGAAGCATGTGGTTGATGTCACANACACGTGAGGAAAGATGT
GNA
3' Read Nucleotide Sequence
>OriGene 3' read for NM_175867 unedited
CCTTGCGCCATTGGCGATGGTCAACTTGCCAGGGCCAGGAAAGCACTGGGGNAGGGTCAC
AGGGCTGCCACCCGGGTTCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTATAGTC
GAGTTTTTTTTTTTTTTTTTTTTTTTTCTTTGTTCAAAGAAAAGTCTTTTTCTTCACAGT
ATAGTGACTCTTTTATAAAGAGGAAGTGAGTTCTGTTGAAAAATACTTGAAATTTTCTCT
TAGGGGGAAAAAGCATTTCTTCACCAGCTTGGTGGGCCACTTGGCCGCGAGCTTCGAGCT
CTGCTTGTTCTGGGCCAGCAGGGACATTTCTTCTTCCGAAACCAGAGCCCAGTGCCTGCT
CCTTATGGCTGGGATGTTGCTCCACACGCGGACAGCATTCTGCAAGGCTCCGCCGTGGAC
ATCTGGGATGGTGACTGGCTCCCTCTCCAGGAAGCGAGATGCGACGTCCAGGTCTTCCTT
GTTCAGCACCAGATTGTCCACGAACATCCAAAAAAAGGGCCCGGGGCTGCCTGGCTTGGG
CCGTGCGTACTGCAGGAGCCGGTGGAACTGGAACAGGTACCAGCTGGGAGGACGGTCACA
GGTGTGGCCCAGGGAGAGGTGTGGCGCCGTACACAATATCGAAGGGTCCCCACTCCTCCA
CATCCTTCCTCAGTGTGTCTGTGACATCAACCACATGCTTCAGTTGTCCCGGGTCAAATC
CACTTTCCAAAAAGCCCAAACTCGTCAGCTCTTTCTTGATGTCTTCAAAAAGGGACAGCA
CCCGGACTGGCTGTCTCCTCCCACAGGCACGGTTTCAAACTCTAAGGGGATTCTCGACTC
TCGGTCGTAAAGGCCTTGAGCTGGCTGCNCCACTCCTCCGAGCTGCACAGCCCGTTCGGG
AGGACG
Restriction Sites Please inquire |
ACCN NM_175867
Insert Size 1300 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_175867.1, NP_787063.1
RefSeq Size 1702 bp
RefSeq ORF 1161 bp
Locus ID 29947
UniProt ID Q9UJW3
Cytogenetics 21q22.3
Protein Families Druggable Genome, Transcription Factors
Protein Pathways Cysteine and methionine metabolism, Metabolic pathways
Summary CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases, but is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, it does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jul 2012
Transcript Variant: This variant (2) uses an alternate in-frame acceptor splice site at the 3' terminal exon compared to variant 1, which results in an isoform (2) that is 1 aa shorter than isoform 1.
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Documents
Resources
"NM_175867" in other vectors (6)
SKU Description Size Price
RC219008 DNMT3L (Myc-DDK-tagged)-Human DNA (cytosine-5-)-methyltransferase 3-like (DNMT3L), transcript variant 2 10 ug
$457.00
RC219008L1 Lenti ORF clone of Human DNA (cytosine-5-)-methyltransferase 3-like (DNMT3L), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC219008L2 Lenti ORF clone of Human DNA (cytosine-5-)-methyltransferase 3-like (DNMT3L), transcript variant 2, mGFP tagged 10 ug
$757.00
RC219008L3 Lenti ORF clone of Human DNA (cytosine-5-)-methyltransferase 3-like (DNMT3L), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC219008L4 Lenti ORF clone of Human DNA (cytosine-5-)-methyltransferase 3-like (DNMT3L), transcript variant 2, mGFP tagged 10 ug
$757.00
RG219008 DNMT3L (tGFP-tagged) - Human DNA (cytosine-5-)-methyltransferase 3-like (DNMT3L), transcript variant 2 10 ug
$489.00 $657.00

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