GMEB1 (NM_024482) Human Untagged Clone

SKU SC110190

GMEB1 (untagged)-Human glucocorticoid modulatory element binding protein 1 (GMEB1), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: GMEB1
• Synonyms: P96PIF; PIF96
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_024482, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGCTAATGCAGAAGTGAGTGTCCCAGTGGGGGATGTGGTTGTGGTACCTACTGAAGGAAATGAAGGGG; AGAATCCTGAAGACACTAAAACCCAAGTGATTTTGCAGTTACAGCCTGTGCAACAAGGGATTTATGAAGC; TGGGTCGGAGAACAACACGGCAGTTGTAGCAGTAGAAACTCACACGATACACAAAATTGAAGAAGGGATT; GATACAGGCACTATAGAAGCAAATGAGGATATGGAAATTGCTTACCCCATAACTTGTGGGGAGAGCAAAG; CCATCCTCCTCTGGAAGAAGTTTGTATGTCCAGGAATAAACGTGAAGTGTGTCAAGTTCAATGATCAGTT; GATCAGCCCCAAGCACTTTGTTCATCTGGCTGGCAAGTCCACTCTGAAGGACTGGAAGAGAGCTATTCGT; CTGGGTGGGATCATGCTCAGGAAAATGATGGACTCCGGACAGATTGATTTTTACCAACATGACAAAGTTT; GCTCCAATACCTGCAGAAGCACCAAATTTGATCTTCTGATCAGCAGTGCAAGAGCTCCAGTGCCAGGACA; GCAGACAAGTGTGGTGCAGACACCCACTTCGGCTGATGGTAGCATCACGCAGATTGCCATCTCAGAAGAG; AGCATGGAAGAGGCAGGGCTGGAATGGAACTCAGCTCTCACCGCTGCTGTCACCATGGCCACGGAGGAGG; GTGTAAAGAAAGACTCAGAGGAAATTTCAGAGGACACTTTGATGTTCTGGAAAGGAATAGCTGATGTAGG; GCTGATGGAAGAGGTTGTCTGCAATATACAGAAGGAAATAGAGGAGCTACTCAGGGGAGTTCAGCAGCGG; CTCATCCAGGCTCCCTTCCAAGTCACAGATGCTGCTGTTCTCAACAATGTAGCACACACATTTGGCCTAA; TGGACACAGTCAAGAAGGTTTTAGACAACAGAAGGAACCAAGTAGAGCAGGGAGAAGAACAGTTTCTCTA; TACTCTGACAGACTTGGAACGCCAGTTGGAGGAGCAGAAGAAGCAAGGCCAGGATCACAGGCTGAAATCT; CAGACAGTTCAAAATGTGGTACTGATGCCTGTGAGCACTCCTAAGCCTCCAAAAAGGCCCCGGCTCCAGC; GGCCAGCCTCCACCACTGTCTTGAGCCCTTCTCCTCCTGTCCAGCAGCCTCAGTTCACAGTCATCTCACC; CATCACCATCACCCCAGTGGGTCAGTCATTTTCCATGGGCAATATTCCAGTGGCCACCCTCAGCCAGGGC; TCCAGTCCTGTGACTGTCCACACACTGCCTTCTGGCCCTCAGCTCTTCCGCTATGCCACAGTGGTCTCCT; CTGCCAAGAGCAGCTCACCAGACACAGTGACCATCCACCCTTCATCTAGCTTGGCGCTGCTGAGCTCTAC; TGCCATGCAGGATGGGAGTACACTGGGCAACATGACCACCATGGTTAGCCCTGTGGAATTGGTGGCCATG; GAGTCCGGCCTAACCTCGGCAATTCAGGCTGTTGAAAGCACCTCAGAGGATGGGCAGACCATCATTGAGA; TTGATCCAGCCCCGGACCCAGAAGCTGAAGATACTGAGGGCAAAGCAGTCATCTTGGAGACAGAGCTGAG; GACTGAGGAGAAAGTTGTGGCTGAGATGGAAGAACACCAGCATCAAGTTCACAATGTGGAGATTGTGGTC; TTAGAGGATTAA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_024482 unedited; GTTCAAATTTGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGCAGAAGTGAG; TGTCCCAGTGGGGGATGTGGTTGTGGTACCTACTGAAGGAAATGAAGGGGAGAATCCTGA; AGACACTAAAACCCAAGTGATTTTGCAGTTACAGCCTGTGCAACAAGGTTTGTTTATCGA; TGGACACTTTTACAACAGGATTTATGAAGCTGGGTCGGAGAACAACACGGCAGTTGTAGC; AGTAGAAACTCACACGATACACAAAATTGAAGAAGGGATTGATACAGGCACTATAGAAGC; AAATGAGGATATGGAAATTGCTTACCCCATAACTTGTGGGGAGAGCAAAGCCATCCTCCT; CTGGAAGAAGTTTGTATGTCCAGGAATAAACGTGAAGTGTGTCAAGTTCAATGATCAGTT; GATCAGCCCCAAGCACTTTGTTCATCTGGCTGGCAAGTCCACTCTGAAGGACTGGAAGAG; AGCTATTCGTCTGGGTGGGATCATGCTCAGGAAAATGATGGACTCCGGACAGATTGATTT; TTACCAACATGACAAAGTTTGCTCCAATACCTGCAGAAGCACCAAATTTGATCTTCTGAT; CAGCAGTGCAAGAGCTCCAGTGCCAGGACAGCAGACAAGTGTGGTGCAGACACCCACTTC; GGCTGATGGTAGCATCACGCAGATTGCCATCTCAGAAGAGAGCATGGAAGAAGCAGGGCT; GGAATGGAACTCAGCTCTCACCGCTGCTGTCACCATGGCCACGGAGGAAGGGTGTAAAGA; AAGACTCAGAGGAAATTTCAGAGGACACTTTGATGTTCTGGAAAGGAATANCTGATGTAN; GGCTGATGGAAGAAGNTGTCTGCATATACAGAAGGAAAATAGAGAGCTACTCAGGGGAGT; TCAGCAGCGGCTCATCCCAGCTCCCTTCC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_024482 unedited; ATCTAGAGTCGAGNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCAGGAAGGCAGCTTG; TTTTGTCCATTTTTCAATGAGGGAGTGGGAAGAACCCAACATTTTCAGTTACAACAATAA; AATTTTGTTTTTTTTTTTTTAAAAAAGGGTCCTATGTGGAAATGAGTGGGACAATGATAA; AAATAAACAAATAATTAAAATTCCAAATCAAAACATAACTCCTGGCCCTGAGATCCCCAG; TTAATCCTCTAAGACCACAATCTCCACATTGTGAACTTGATGCTGGGGTTCTTCCATCTC; AGCCACAACTTTCTCCTCAGTCCTCAGCTCTGTCTCCAAGATGACTGCTTTGCCCTCAGT; ATCTTCAGTTTCTGGGACCGGGGCTGGATCAATCTCAATGATGGTCTGCCCATCCTTTGA; GGTGCTTTCAACAGCCTGAATTGCCGAGGTTAGGCCGGACTCCATGGCCACCAATTCCAC; AGGGCTAACCATGGTGGTCATGTTGCCCAACGTACCTCCATCCTGCATGGCAGTAAAGCT; CACCACCGCCAAACTAGATGAAGGGTGCATGGCCACTGTGTTTGGAGAGCTGTTCTTTGG; AAAAGAGACCACTGTGGCATAACGCAAAAACTGAGGCCCCAAAGGACATGTGTGGAACAG; TCCAGAGACTGGACCCCTGGCTGAGGTTGCCCACTGAAAAATTGCCCATGAAAAATGACT; GCCCCCATTGGGGCGATGGGGATGGCGTGAAAGAACGTCAACCGGAGCTGCTGTTAAAGA; AGAAAACAGCCTAATAAAGTGGCGGAAGCTTGCCCCTGTGAACCGGCCCCTTTTGGAGGT; CTAGAGCGCCACAGGCTCCACCCCAATTTTGAACGGGCGGAGAATTCACCCGGGACCCGG; CCCTGCTCTTTTGTTCCCCCATGGGGGTCCAACGTGTGAAATTAAAAAAAAGGGCTTTCC; CCCGGCTTATTTGGTCTCTTTGTTGGCTAAAACTCTTGAATCGGCCCTTAGCGCAAAAGG; G;
• Restriction Sites: NotI-NotI
• ACCN: NM_024482
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_024482.1, NP_077808.1
• RefSeq Size: 1944 bp
• RefSeq ORF: 1692 bp
• Locus ID: 10691
• UniProt ID: Q9Y692
• Cytogenetics: 1p35.3
• Domains: SAND
• Protein Families: Druggable Genome, Transcription Factors
• Summary: This gene encodes a member of KDWK gene family which associates with GMEB2 protein. The GMEB1-GMEB2 complex is essential for parvovirus DNA replication. Studies in rat for a similar gene suggest that this gene's role is to modulate the transactivation of the glucocorticoid receptor when it is bound to glucocorticoid response elements. Three alternative spliced transcript variants encoding different isoforms exist. [provided by RefSeq, Feb 2016]; Transcript Variant: This variant (2) differs in the 5' UTR and uses an alternate in-frame splice junction compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Variants 2 and 3 both encode the same isoform (2).