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EED (NM_152991) Human Untagged Clone

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SKU
SC109981
EED (untagged)-Human embryonic ectoderm development (EED), transcript variant 2
  $457.00
4 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol EED
Synonyms COGIS; HEED; WAIT1
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_152991, the custom clone sequence may differ by one or more nucleotides


ATGTCCGAGAGGGAAGTGTCGACTGCGCCGGCGGGAACAGACATGCCTGCGGCCAAGAAGCAGAAGCTGA
GCAGTGACGAGAACAGCAATCCAGACCTCTCTGGAGACGAGAATGATGACGCTGTCAGTATAGAAAGTGG
TACAAACACTGAACGCCCTGATACACCTACAAACACGCCAAATGCACCTGGAAGGAAAAGTTGGGGAAAG
GGAAAATGGAAGTCAAAGAAATGCAAATATTCTTTCAAATGTGTAAATAGTCTCAAGGAAGATCATAACC
AACCATTGTTTGGAGTTCAGTTTAACTGGCACAGTAAAGAAGGAGATCCATTAGTGTTTGCAACTGTAGG
AAGCAACAGAGTTACCTTGTATGAATGTCATTCACAAGGAGAAATCCGGTTGTTGCAATCTTACGTGGAT
GCTGATGCTGATGAAAACTTTTACACTTGTGCATGGACCTATGATAGCAATACGAGCCATCCTCTGCTGG
CTGTAGCTGGATCTAGAGGCATAATTAGGATAATAAATCCTATAACAATGCAGTGTATAAAGCACTATGT
TGGCCATGGAAATGCTATCAATGAGCTGAAATTCCATCCAAGAGATCCAAATCTTCTCCTGTCAGTAAGT
AAAGATCATGCTTTACGATTATGGAATATCCAGACGGACACTCTGGTGGCAATATTTGGAGGCGTAGAAG
GGCACAGAGATGAAGTTCTAAGTGCTGATTATGATCTTTTGGGTGAAAAAATAATGTCCTGTGGTATGGA
TCATTCTCTTAAACTTTGGAGGATCAATTCAAAGAGAATGATGAATGCAATTAAGGAATCTTATGATTAT
AATCCAAATAAAACTAACAGGCCATTTATTTCTCAGAAAATCCATTTTCCTGATTTTTCTACCAGAGACA
TACATAGGAATTATGTTGATTGTGTGCGATGGTTAGGCGATTTGATACTTTCTAAGTCTTGTGAAAATGC
CATTGTGTGCTGGAAACCTGGCAAGATGGAAGATGATATAGATAAAATTAAACCCAGTGAATCTAATGTG
ACTATTCTTGGGCGATTTGATTACAGCCAGTGTGACATTTGGTACATGAGGTTTTCTATGGATTTCTGGC
AAAAGATGCTTGCATTGGGCAATCAAGTTGGCAAACTTTATGTTTGGGATTTAGAAGTAGAAGATCCTCA
TAAAGCCAAGTAA

5' Read Nucleotide Sequence
>OriGene 5' read for NM_152991 unedited
GGATTTTGTAATACGACTTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGAAGCCGCG
CGGGAGGGCGCGCGCGCGCGCCCCTTTTTCAGCAGTGTGGCGGGGTCGCACGCACGCCCG
CCTCGGCGGCTGGGCGCGATTTGCGACAGTGGGGGGGGCGGTGGAGGTGGCGGCGGCAGC
GGCAACTTTGCGGCAAGCTCGGGCCGGGCTTGCTTGACGGCGGTGTGGCGGAGGCCCCGC
CCCAGGCGGCAGGAACCTGGAGGGAGGCGGAGGAATATGTCCGAGAGGGAAGTGTCGACT
GCGCCGGCGGGAACAGACATGCCTGCGGCCAAGAAGCAGAAGCTGAGCAGTGACGAGAAC
AGCAATCCAGACCTCTCTGGAGACGAGAATGATGACGCTGTCAGTATAGAAAGTGGTACA
AACACTGAACGCCCTGATACACCTACAAACACGCCAAATGCACCTGGAAGGAAAAGTTGG
GGAAAGGGAAAATGGAAGTCAAAGAAATGCAAATATTCTTTCAAATGTGTAAATAGTCTC
AAGGAAGATCATAACCAACCATTGTTTGGAGTTCAGTTTAACTGGCACAGTAAAGAAGGA
GATCCATTAGTGTTTGCAACTGTAGGAAGCAACAGAGTTACCTTGTATGAATGTCATTCA
CAAGGAGAAATCCGGTTGTTGCAATCTTACGTGGATGCTGATGCTGATGAAAACTTTTAC
ACTTGTGCATGGACCTATGATAGCAATACGAGCCATCCTCTGCTGGCTGTAGCTGGATCT
AGAGGCATAATTAGGATAATAAATCCTATNACAATGCAGTGTATAAAGCACTATGTTGGC
CCATGAAATGCTANTCATGAGCTGAAATTCATNNCAGAGATCCAAATCTCTNCTGTCAGT
AGTAAAAGATCATGCTTACGATNATGGAATATCCAGACGACACTCTGTGGCATATTNGNA
GCGTANAAGGCACCNAATGAGTNCTAGTGCTGATATGT
3' Read Nucleotide Sequence
>OriGene 3' read for NM_152991 unedited
AGGANAGCACTGGGGNAGGGTCAAGGGATGCCACCCGGGTATCTGTTCAGGAAAACAGCT
ATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTACAAAAATAAATTT
TTATTAAAAGCAGTAGAGTCTGAGCAGGAAGACAGTACAAAGAATGTAAACAATGTAAAC
ATCACTACATTCAGCTCAGCCTGATTGAATGCTGAAAGACAACTCTAAATGCTCTACGTG
CCCTTACTAGCAAGATACATTAATTCTATTTTACACAGACAACAAACACACTCTAATTTT
GATTAGGCAAAAGTATTTTATCGAAGTCGATCCCAGCGCCAAATACTGGCATCATCACAA
ACAGCTATAAGAATGCTGCTATCCCTGCTAAAACTGGTTTGTCGAATAGCAGCACCACAT
TTATGATGAGTCAGTGTTGTACATTTGGCTTTATGAGGATCTTCTACTTCTAAATCCCAA
ACATAAAGTTTGCCAACTTGATTGCCCAATGCAAGCATCTTTTGCCAGAAATCCATAGAA
AACCTCATGTACCAAATGTCACACTGGCTGTAATCAAATCGCCCAAGAATAGTCACATTA
GATTCACTGGGTTTAATTTTATCTATATCATCTTCCATCTTGCCAGGTTTCCAGCACACA
ATGGCATTTTCACAAGACTTAGAAAGTATCAAATCGCCTAACCATCGCACACAATCAACA
TAATTCCTATGTATGTCTCTGGTAGAAAATCAGGAAAAGGATTTTCTGAGAAATAATGGC
CTGTTAGTTTATTTGGATTATAACAATAGATTCTTAATTGCATTCTCATTCTCTTTGGAT
TGATCCTNCAAGTTTAAGAGATGAT
Restriction Sites NotI-NotI |
ACCN NM_152991
Insert Size 1900 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_152991.1, NP_694536.1
RefSeq Size 2413 bp
RefSeq ORF 1203 bp
Locus ID 8726
Cytogenetics 11q14.2
Domains WD40
Protein Families Druggable Genome, Transcription Factors
Summary This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. Two transcript variants encoding distinct isoforms have been identified for this gene. provided by RefSeq, Jul 2008
Transcript Variant: This variant (2) contains an additional segment within the coding region, which results in immediate translation termination, compared to variant 1. The resulting isoform (b) contains a shorter C-terminus compared to isoform a.
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Documents
Resources
"NM_152991" in other vectors (6)
SKU Description Size Price
RC224366 EED (Myc-DDK-tagged)-Human embryonic ectoderm development (EED), transcript variant 2 10 ug
$457.00
RC224366L1 Lenti ORF clone of Human embryonic ectoderm development (EED), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC224366L2 Lenti ORF clone of Human embryonic ectoderm development (EED), transcript variant 2, mGFP tagged 10 ug
$757.00
RC224366L3 Lenti ORF clone of Human embryonic ectoderm development (EED), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC224366L4 Lenti ORF clone of Human embryonic ectoderm development (EED), transcript variant 2, mGFP tagged 10 ug
$757.00
RG224366 EED (tGFP-tagged) - Human embryonic ectoderm development (EED), transcript variant 2 10 ug
$489.00 $657.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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