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PPAP2A (PLPP1) (NM_003711) Human Untagged Clone

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SKU
SC109962
PPAP2A (untagged)-Human phosphatidic acid phosphatase type 2A (PPAP2A), transcript variant 1
  $300.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PPAP2A
Synonyms LLP1a; LPP1; PAP-2a; PAP2; PPAP2A
Vector PCMV6-Neo
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>OriGene ORF sequence for NM_003711 edited
ATGTTTGACAAGACGCGGCTGCCGTACGTGGCCCTCGATGTGCTCTGCGTGTTGCTGGCT
GGATTGCCTTTTGCAATTCTTACTTCAAGGCATACCCCCTTCCAACGAGGAGTATTCTGT
AATGATGAGTCCATCAAGTACCCTTACAAAGAAGACACCATACCTTATGCGTTATTAGGT
GGAATAATCATTCCATTCAGTATTATCGTTATTATTCTTGGAGAAACCCTGTCTGTTTAC
TGTAACCTTTTGCACTCAAATTCCTTTATCAGGAATAACTACATAGCCACTATTTACAAA
GCCATTGGAACCTTTTTATTTGGTGCAGCTGCTAGTCAGTCCCTGACTGACATTGCCAAG
TATTCAATAGGCAGACTGCGGCCTCACTTCTTGGATGTTTGTGATCCAGATTGGTCAAAA
ATCAACTGCAGCGATGGTTACATTGAATACTACATATGTCGAGGGAATGCAGAAAGAGTT
AAGGAAGGCAGGTTGTCCTTCTATTCAGGCCACTCTTCGTTTTCCATGTACTGCATGCTG
TTTGTGGCACTTTATCTTCAAGCCAGGATGAAGGGAGACTGGGCAAGACTCTTACGCCCC
ACACTGCAATTTGGTCTTGTTGCCGTATCCATTTATGTGGGCCTTTCTCGAGTTTCTGAT
TATAAACACCACTGGAGCGATGTGTTGACTGGACTCATTCAGGGAGCTCTGGTTGCAATA
TTAGTTGCTGTATATGTATCGGATTTCTTCAAAGAAAGAACTTCTTTTAAAGAAAGAAAA
GAGGAGGACTCTCATACAACTCTGCATGAAACACCAACAACTGGGAATCACTATCCGAGC
AATCACCAGCCTTGA
5' Read Nucleotide Sequence
>OriGene 5' read for NM_003711 unedited
GTGGGTTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGGCACGAGGGGGCCCCGGGC
GCCATTGCTGGCGGTGGGAGCGCCGCCCGGTCTCAGCCCGCCCTCGGCTGCTCTCCTCCT
CCGGCTGGGAGGGGCCGTAGCTCGGGGCCGTCGCCAGCCCCGGCCCGGGCTCGAGAATCA
AGGGCCTCGGCCGCCGTCCCGCAGCTCAGTCCATCGCCCTTGCCGGGCAGCCCGGGCAGA
GACCATGTTTGACAAGACGCGGCTGCCGTACGTGGCCCTCGATGTGCTCTGCGTGTTGCT
GGCTGGATTGCCTTTTGCAATTCTTACTTCAAGGCATACCCCCTTCCAACGAGGAGTATT
CTGTAATGATGAGTCCATCAAGTACCCTTACAAAGAAGACACCATACCTTATGCGTTATT
AGGTGGAATAATCATTCCATTCAGTATTATCGTTATTATTCTTGGAGAAACCCTGTCTGT
TTACTGTAACCTTTTGCACTCAAATTCCTTTATCAGGAATAACTACATAGCCACTATTTA
CAAAGCCATTGGAACCTTTTTATTTGGTGCAGCTGCTAGTCAGTCCCTGACTGACATTGC
CAAGTATTCAATAGGCAGACTGCGGCCTCACTTCTTGGATGTTTGTGATCCAGATTGGTC
AAAAATCAACTGCAGCGATGGTTACATTGAATACTACATATGTCGAGGGAATGCAGAAAG
AGTTAAAGAAAGCAGGTTGTCCTTCTATTCAGCCACTCTTCGTTTTCCATGTACTGCATG
CTGTTTGTGGCACTTTATCTTCAAGCCAGGATGAAGGAGACTGGGCAAGACTCTTACGCC
CCACACTGCATTTGGTCTTGTTGCCGTATCCATTTATTGTGGCCCTTTCTCGAGTTTCTG
ATTATAAACACACTGGAGCGATGTGTTGACTGACCTCATTCAGGGAGGCTTCTGGTTGCA
ATTATTAGTGGCTTGTTATTATTGTATATCGAT
Restriction Sites Please inquire |
ACCN NM_003711
Insert Size 1530 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003711.2, NP_003702.2
RefSeq Size 1638 bp
RefSeq ORF 855 bp
Locus ID 8611
UniProt ID O14494
Cytogenetics 5q11.2
Domains acidPPc
Protein Families Druggable Genome, Transmembrane
Protein Pathways Ether lipid metabolism, Fc gamma R-mediated phagocytosis, Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways, Sphingolipid metabolism
Summary The protein encoded by this gene is a member of the phosphatidic acid phosphatase (PAP) family. PAPs convert phosphatidic acid to diacylglycerol, and function in synthesis of glycerolipids and in phospholipase D-mediated signal transduction. This enzyme is an integral membrane glycoprotein that plays a role in the hydrolysis and uptake of lipids from extracellular space. Alternate splicing results in multiple transcript variants of this gene. provided by RefSeq, May 2013
Transcript Variant: This variant (1, also named PAP2-a1) lacks an alternate exon and contains an in-frame alternate exon in the 5' coding region, compared to variant 2. The resulting isoform (1) contains a distinct internal segment and is shorter, compared to isoform 2.
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Documents
Resources
"NM_003711" in other vectors (4)
SKU Description Size Price
RC208064 PPAP2A (Myc-DDK-tagged)-Human phosphatidic acid phosphatase type 2A (PPAP2A), transcript variant 1 10 ug
$300.00
RC208064L3 Lenti ORF clone of Human phosphatidic acid phosphatase type 2A (PPAP2A), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC208064L4 Lenti ORF clone of Human phosphatidic acid phosphatase type 2A (PPAP2A), transcript variant 1, mGFP tagged 10 ug
$600.00
RG208064 PPAP2A (tGFP-tagged) - Human phosphatidic acid phosphatase type 2A (PPAP2A), transcript variant 1 10 ug
$489.00 $500.00

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