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TNXB (NM_032470) Human Untagged Clone

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SKU
SC109841
TNXB (untagged)-Human tenascin XB (TNXB), transcript variant XB-S
  $617.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol TNXB
Synonyms EDS3; EDSCLL; EDSCLL1; HXBL; TENX; TN-X; TNX; TNXB1; TNXB2; TNXBS; VUR8; XB; XBS
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC109841 sequence for NM_032470 edited (data generated by NextGen Sequencing)
ATGCGCCTCTCGTGGAGCGTGGCCCAGGGCCCCTTTGATTCCTTCGTGGTCCAGTATGAG
GACACGAACGGGCAGCCCCAGGCCTTGCTCGTGGACGGCGACCAGAGCAAGATCCTCATC
TCAGGCCTGGAGCCCAGCACCCCCTACAGGTTCCTCCTCTATGGCCTCCATGAAGGGAAG
CGCCTGGGGCCCCTCTCAGCTGAGGGCACCACAGGGCTGGCTCCTGCTGGTCAGACCTCA
GAGGAGTCAAGGCCCCGCCTGTCCCAGCTGTCTGTGACTGACGTGACCACCAGTTCACTG
AGGCTCAACTGGGAGGCCCCACCGGGGGCCTTCGACTCCTTCCTGCTCCGCTTTGGGGTT
CCATCACCAAGCACACTGGAGCCGCATCCGCGTCCACTGCTGCAGCGCGAGCTGATGGTG
CCGGGGACGCGGCACTCGGCCGTGCTCCGGGACCTGCGTTCCGGGACTCTGTACAGCCTG
ACACTGTATGGGCTGCGAGGACCCCACAAGGCCGACAGCATCCAGGGAACCGCCCGCACC
CTCAGCCCAGTTCTGGAGAGCCCCCGTGACCTCCAATTCAGTGAAATCAGGGAGACCTCA
GCCAAGGTCAACTGGATGCCCCCACCATCCCGGGCGGACAGCTTCAAAGTCTCCTACCAG
CTGGCGGACGGAGGGGAGCCTCAGAGTGTGCAGGTGGATGGCCAGGCCCGGACCCAGAAA
CTCCAGGGGCTGATCCCAGGCGCTCGCTATGAGGTGACCGTGGTCTCGGTCCGAGGCTTT
GAGGAGAGTGAGCCTCTCACAGGCTTCCTCACCACGGTTCCTGACGGTCCCACACAGTTG
CGTGCACTGAACTTGACCGAGGGATTCGCCGTGCTGCACTGGAAGCCCCCCCAGAATCCT
GTAGACACCTATGACATCCAGGTCACAGCCCCTGGGGCCCCGCCTCTGCAGGCGGAGACC
CCAGGCAGCGCGGTGGACTACCCCCTGCATGACCTTGTCCTCCACACCAACTACACCGCC
ACAGTGCGTGGCCTGCGGGGCCCCAACCTCACTTCCCCAGCCAGCATCACCTTCACCACA
GGGCTAGAGGCCCCTCGGGACTTGGAGGCCAAGGAAGTGACCCCCCGCACCGCCCTGCTC
ACTTGGACTGAGCCCCCAGTCCGGCCCGCAGGCTACCTGCTCAGCTTCCACACCCCTGGT
GGACAGACCCAGGAGATCCTGCTCCCAGGAGGGATCACATCTCACCAGCTCCTTGGCCTC
TTTCCCTCCACCTCCTACAATGCACGGCTCCAGGCCATGTGGGGCCAGAGCCTCCTGCCG
CCCGTGTCCACCTCTTTCACCACGGGTGGGCTGCGGATCCCCTTCCCCAGGGACTGCGGG
GAGGAGATGCAGAACGGAGCCGGTGCCTCCAGGACCAGCACCATCTTCCTCAACGGCAAC
CGCGAGCGGCCCCTGATCGTGTTTTGCGACATGGAGACTGATGGGGGCGGCTGGCTGGTG
TTCCAGCGCCGCATGGATGGACAGACAGACTTCTGGAGGGACTGGGAGGACTATGCCCAT
GGTTTTGGGAACATCTCTGGAGAGTTCTGGCTGGGCAATGAGGCCCTGCACAGCCTGACA
CAGGCAGGTGACTACTCCATGCGCGTGGACCTGCGGGCTGGGGACGAGGCTGTGTTCGCC
CAGTACGACTCCTTCCACGTAGACTCGGCTGCGGAGTACTACCGCCTCCACTTGGAGGGC
TACCACGGCACCGCAGGGGACTCCATGAGCTACCACAGCGGCAGTGTCTTCTCTGCCCGT
GATCGGGACCCCAACAGCTTGCTCATCTCCTGCGCTGTCTCCTACCGAGGGGCCTGGTGG
TACAGGAACTGCCACTACGCCAACCTCAACGGGCTCTACGGGAGCACAGTGGACCATCAG
GGAGTGAGCTGGTACCACTGGAAGGGCTTCGAGTTCTCGGTGCCCTTCACGGAAATGAAG
CTGAGACCAAGAAACTTTCGCTCCCCAGCGGGGGGAGGCTGA

Clone variation with respect to NM_032470.3
375 t=>a;903 g=>a;916 g=>a;1208 a=>c;1457 a=>t
5' Read Nucleotide Sequence
>OriGene 5' read for NM_032470 unedited
NNCCCNNNCCCCNNNNNCCNNNNNNCCTGTCAGATTTGTATACGATCATATAGGCGGCCG
CGNAATTCGCACCAGCTGGGNATGACAGCCCCANAAAAGGACACACCAGCCCCAGAGTTA
GCCCCAGAGGCCCCTGAGCCTCCTGAAGAGCCCCGCCTAGGAGTGCTGACCGTGACCGAC
ACAACCCCAGACTCCATGCGCCTCTCGTGGAGCGTGGCCCAGGGCCCCTTTGATTCCTTC
GTGGTCCAGTATGAGGACACGAACGGGCAGCCCCAGGCCTTGCTCGTGGACGGCGACCAG
AGCAAGATCCTCATCTCAGGCCTGGAGCCCAGCACCCCCTACAGGTTCCTCCTCTATGGC
CTCCATGAAGGGAAGCGCCTGGGGCCCCTCTCAGCTGAGGGCACCACAGGGCTGGCTCCT
GCTGGTCAGACCTCAGAGGAGTCAAGGCCCCGCCTGTCCCAGCTGTCTGTGACTGACGTG
ACCACCAGTTCACTGAGGCTCAACTGGGAGGCCCCACCGGGGGCCTTCGACTCCTTCCTG
CTCCGCTTTGGGGTTCCATCACCAAGCACACTGGAGCCGCATCCGCGTCCACTGCTGCAG
CGCGAGCTGATGGTGCCGGGGACGCGGCACTCGGCCGTGCTCCGGGACCTGCGTTCCGGG
ACTCTGTACAGCCTGACACTGTATGGGCTGCGAGGACCCCACANGGCCGACAGCATCCAG
GGAACCGNCCGCACCCTCAGCCCAGTTCTGGGAGAGCCCNCGTGACCTCCATTNCAGTGA
AATCAGGGAGACCTCAGCCAAGGTCAACTGGATGCCCCCACATCCCGGGCGACAGCTTNC
AAGTCTCCTACCGCTGGCGNACGNAGGGGAGCCTCANATGTGCCAGTGGATGGCCANNGC
CCGNACCAGAACTCCAGGGCCTGA
3' Read Nucleotide Sequence
>OriGene 3' read for NM_032470 unedited
GGGGGGGGNNNNNNNNNNNNTTTTNNNNNAANAAAATCTTGNACCGCGCCGCATACTANG
ATCGGTTTTTTTTTTTTTTTTTTTACCTCAGTTTCTCCTTTATTGCTCCCGTACGAACCG
CTCCCCTCCCCCCTGTAAACACAGTGCTGCGAGATCGCTGGCAGAGAAGGCTTCCTCCAG
CGGCTGGGTGGTGAAGGACCCTGGCTCTTCTCTCGGGGCGACCCCTCAGTGCTCGGCAGT
CATACTGGGGTGCGAGAGAGGTGGGCAGCAGCTCAGCCTCCCCCCGCTGGGGAGCGAAAG
TTTCTTGGTCTCAGCTTCATTTCCGTGAAGGGCACCGAGAACTCGAAGCCCTTCCAGTGG
TACCAGCTCACTCCCTGATGGTCCACTGTGCTCCCGTAGAGCCCGTTGAGGTTGGCGTAG
TGGCAGTTCCTGTACCACCAGGCCCCTCGGTAGGAGACAGCGCAGGAGATGAGCAAGCTG
TTGGGGTCCCGATCACGGGCAGAGAAGACACTGCCGCTGTGGTAGCTCATGGAGTCCCCT
GCGGTGCCGTGGTAGCCCTCCAAGTGGAGGCGGTAGTACTCCGCAGCCGAGTCTACGTGG
AAGGAGTCGTACTGGGCGAACACAGCCTCGTCCCCAGCCCGCAGGTCCACGCGCATGGAG
TAGTCACCTGCCTGTGTCAGGCTGTGCAGGNCCTCATTGCCCAGCCAGACTCTCCAGAGA
TGTTCCCAAAAACATGGNCATAGTCCTCCCAGTCCCTCCAGAAGTCTGTCTGTCCATCCA
TTGCGCGCTGGGACACCAGCCAGCCCGCCCCATCAGTCTCCATGTCGCAAACACGATCAN
GGGCCCGCTCGCGTTTGCGTTGAGGAAAATGTGCCTGTCCTGNAGCCACCGGTCCGTCTG
CATCTCTNCCGCAGTCTGGGGAAGGGGATCGCACCCACCCGTGTGAAAGAGGTGGACCCG
GCGNNNAGAGCTCTGGCCCCATGCCTGGAACTGCATTGGAGAGGGG
Restriction Sites NotI-NotI |
ACCN NM_032470
Insert Size 2390 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032470.2, NP_115859.2
RefSeq Size 3129 bp
RefSeq ORF 2022 bp
Locus ID 7148
UniProt ID P22105
Cytogenetics 6p21.33-p21.32
Domains FBG, FN3
Protein Families Druggable Genome, Secreted Protein
Protein Pathways ECM-receptor interaction, Focal adhesion
Summary This gene encodes a member of the tenascin family of extracellular matrix glycoproteins. The tenascins have anti-adhesive effects, as opposed to fibronectin which is adhesive. This protein is thought to function in matrix maturation during wound healing, and its deficiency has been associated with the connective tissue disorder Ehlers-Danlos syndrome. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. It is one of four genes in this cluster which have been duplicated. The duplicated copy of this gene is incomplete and is a pseudogene which is transcribed but does not encode a protein. The structure of this gene is unusual in that it overlaps the CREBL1 and CYP21A2 genes at its 5' and 3' ends, respectively. Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jul 2008
Transcript Variant: This variant (XB-S) is transcribed from a cryptic internal promoter sequence and is substantially shorter than variant 3 at the 5' end. It encodes isoform 2, which is identical to the C-terminus of the full-length protein, isoform 3.
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Documents
Resources
"NM_032470" in other vectors (4)
SKU Description Size Price
RC220722 TNXB (Myc-DDK-tagged)-Human tenascin XB (TNXB), transcript variant XB-S 10 ug
$616.00
RC220722L3 Lenti ORF clone of Human tenascin XB (TNXB), transcript variant XB-S, Myc-DDK-tagged 10 ug
$916.00
RC220722L4 Lenti ORF clone of Human tenascin XB (TNXB), transcript variant XB-S, mGFP tagged 10 ug
$916.00
RG220722 TNXB (tGFP-tagged) - Human tenascin XB (TNXB), transcript variant XB-S 10 ug
$789.00 $816.00

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