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FXYD1 (NM_021902) Human Untagged Clone

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SKU
SC109579
FXYD1 (untagged)-Human FXYD domain containing ion transport regulator 1 (FXYD1), transcript variant b
  $150.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol FXYD1
Synonyms PLM
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC109579 sequence for NM_021902 edited (data generated by NextGen Sequencing)
ATGGCGTCTCTTGGCCACATCTTGGTTTTCTGTGTGGGTCTCCTCACCATGGCCAAGGCA
GAAAGTCCAAAGGAACACGACCCGTTCACTTACGACTACCAGTCCCTGCAGATCGGAGGC
CTCGTCATCGCCGGGATCCTCTTCATCCTGGGCATCCTCATCGTGCTGAGCAGAAGATGC
CGGTGCAAGTTCAACCAGCAGCAGAGGACTGGGGAACCCGATGAAGAGGAGGGAACTTTC
CGCAGCTCCATCCGCCGTCTGTCCACCCGCAGGCGGTAG

Clone variation with respect to NM_021902.2
5' Read Nucleotide Sequence
>OriGene 5' read for NM_021902 unedited
TTCGGCACGAGGCTCAGCCCCCGGGGCACAGCAGGACGTTTGGGGGCCTTCTTTCAGCAG
GGGACAGCCCGATTGGGGACAATGGCGTCTCTTGGCCACATCTTGGTTTTCTGTGTGGGT
CTCCTCACCATGGCCAAGGCAGAAAGTCCAAAGGAACACGACCCGTTCACTTACGACTAC
CAGTCCCTGCATATCGGAGGCCTCGTCATCGCCGGGATCCTCTTCATCCTGGGCATCCTC
ATCGTGCTGAGCAGAAGATGCCGGTGCAAGTTCAACCAGCAGCAGAGGACTGGGGAACCC
GATGAAGAGGAGGGAACTTTCCGCAGCTCCATCCGCCGTCTGTCCACCCGCAGGCGGTAG
AAACACCTGGAGCGATGGAATCCGGCCAGGACTCCCCTGGCACCTGACATCTCCCACGCT
CACACTGCGGGCNCCACCGGCCCCTTCCGCGGCCCCTTTCCCAGGCCCTGCCCCCGCAGA
CTCCCCCTGCCGCCAAGACTTCCAATAAAACGTGCGTTCCTCTCGACAAAAAAAAAAAAA
AAAAACTCGACTCTAGATTGCGGCCGCGGTCATAGCTGGTNCCTGAACAGATCCCGGGTG
GCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCC
CACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTA
TATATTATGGGNGGAGGGGGGGGGGGGTTTNNACCNAAGGGGGAATTTGGGAGAAAAAAC
CCTGTAGGGCCCGGGNGGTCTTA
3' Read Nucleotide Sequence
>OriGene 3' read for NM_021902 unedited
CCCCCCCCCCTTAGGTTTGAACCGCGGCTCGCATCCTACGATCGAGTTTTTTTTTTTTTT
TTTTGTCGAGAGGACACGCACGTTTTATTGGAAGTCTTGGCGGCAGGGGGAGTCTGCGGG
GGCAGGGCTGGGGAAGGGGCGGCGGAGGGGGCGGTGGGCGCGCAGGTGGAGCGTGGGAGA
TGTCAGGTGCCAGGGGAGTCCTGGCCGGATTCCATCGCTCCAGGTGTTTCTACCGCCTGC
GGGTGGACAGACGGCGGATGGAGCTGCGGAAAGTTCCCTCCTCTTCATCGGGTTCCCCAG
TCCTCTGCTGCTGGTTGAACTTGCACCGGCATCTTCTGCTCAGCACGATGACGATGCCCA
CGATGAAGAGGATCCCGGCCTTGACTAGGTCTCCGATCTGTTGGGACTGTTTAACTTACG
TGCACGCGCCCGGGCCACTCTTTCCCTCTCGTCCTGTCCCCTTCGTTCGCCACTTTCTCC
TCTCCCCCTCCGCTCCCTCCTCCTCCTTTCTTCTCTTCTCTTTCTTCCTTTTCCCCTCTT
CCCTTTCCTCTCCTCATCATTTCTTCCCCTTCTACTCCCTTTCTCGNCCCCTCCACTCCT
TCTTCTCCCCTCCTTTTTCTCCTTCTCCTCACCTTCTCTATCCTCACCCCTCACTCATCC
CCCCCTCACCCTCTCCTCTAACTCCCCTCTTCTCCTCCCCCTTCCTCTCCCTTTTCCCTT
TCTCACCCTCTTCCTCTCCCTTTTCCATCCCCCTCCCCCTCCCTCCCCTTCTCTCTTCTC
CTTTTCTCTTTCCCCCCCTCTCCTCTCCTATCCCCCCCTTCCATATTCTCCTCTTCTCCC
CTTCCTTTTTTTTTCCCTCTACCCCTTCCCCCTCTTCTCTAATTCCCCTTCTTCTTTTCC
TCTCCCCCCTTATTATCTCTTAN
Restriction Sites NotI-NotI |
ACCN NM_021902
Insert Size 279 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_021902.2, NP_068702.1
RefSeq Size 558 bp
RefSeq ORF 279 bp
Locus ID 5348
UniProt ID O00168
Cytogenetics 19q13.12
Domains ATP1G1_PLM_MAT8
Protein Families Ion Channels: Other, Transmembrane
Summary This gene encodes a member of a family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. The approved human gene nomenclature for the family is FXYD-domain containing ion transport regulator. Mouse FXYD5 has been termed RIC (Related to Ion Channel). FXYD2, also known as the gamma subunit of the Na,K-ATPase, regulates the properties of that enzyme. FXYD1 (phospholemman), FXYD2 (gamma), FXYD3 (MAT-8), FXYD4 (CHIF), and FXYD5 (RIC) have been shown to induce channel activity in experimental expression systems. Transmembrane topology has been established for two family members (FXYD1 and FXYD2), with the N-terminus extracellular and the C-terminus on the cytoplasmic side of the membrane. The protein encoded by this gene is a plasma membrane substrate for several kinases, including protein kinase A, protein kinase C, NIMA kinase, and myotonic dystrophy kinase. It is thought to form an ion channel or regulate ion channel activity. Transcript variants with different 5' UTR sequences have been described in the literature. provided by RefSeq, Jul 2008
Transcript Variant: This variant (b) differs in the 5' UTR compared to variant a. Variants a, b, c and d encode the same protein.
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Documents
Resources
"NM_021902" in other vectors (6)
SKU Description Size Price
RC211575 FXYD1 (Myc-DDK-tagged)-Human FXYD domain containing ion transport regulator 1 (FXYD1), transcript variant b 10 ug
$150.00
RC211575L1 Lenti ORF clone of Human FXYD domain containing ion transport regulator 1 (FXYD1), transcript variant b, Myc-DDK-tagged 10 ug
$450.00
RC211575L2 Lenti ORF clone of Human FXYD domain containing ion transport regulator 1 (FXYD1), transcript variant b, mGFP tagged 10 ug
$450.00
RC211575L3 Lenti ORF clone of Human FXYD domain containing ion transport regulator 1 (FXYD1), transcript variant b, Myc-DDK-tagged 10 ug
$450.00
RC211575L4 Lenti ORF clone of Human FXYD domain containing ion transport regulator 1 (FXYD1), transcript variant b, mGFP tagged 10 ug
$450.00
RG211575 FXYD1 (tGFP-tagged) - Human FXYD domain containing ion transport regulator 1 (FXYD1), transcript variant b 10 ug
$489.00

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