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CD10 (MME) (NM_000902) Human Untagged Clone

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SKU
SC109440
MME (untagged)-Human membrane metallo-endopeptidase (MME), transcript variant 1
  $688.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol CD10
Synonyms CALLA; CD10; CMT2T; NEP; SCA43; SFE
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC109440 sequence for NM_000902 edited (data generated by NextGen Sequencing)
ATGGGCAAGTCAGAAAGTCAGATGGATATAACTGATATCAACACTCCAAAGCCAAAGAAG
AAACAGCGATGGACTCCACTGGAGATCAGCCTCTCGGTCCTTGTCCTGCTCCTCACCATC
ATAGCTGTGACAATGATCGCACTCTATGCAACCTACGATGATGGTATTTGCAAGTCATCA
GACTGCATAAAATCAGCTGCTCGACTGATCCAAAACATGGATGCCACCACTGAGCCTTGT
ACAGACTTTTTCAAATATGCTTGCGGAGGCTGGTTGAAACGTAATGTCATTCCCGAGACC
AGCTCCCGTTACGGCAACTTTGACATTTTAAGAGATGAACTAGAAGTCGTTTTGAAAGAT
GTCCTTCAAGAACCCAAAACTGAAGATATAGTAGCAGTGCAGAAAGCAAAAGCATTGTAC
AGGTCTTGTATAAATGAATCTGCTATTGATAGCAGAGGTGGAGAACCTCTACTCAAACTG
TTACCAGACATATATGGGTGGCCAGTAGCAACAGAAAACTGGGAGCAAAAATATGGTGCT
TCTTGGACAGCTGAAAAAGCTATTGCACAACTGAATTCTAAATATGGGAAAAAAGTCCTT
ATTAATTTGTTTGTTGGCACTGATGATAAGAATTCTGTGAATCATGTAATTCATATTGAC
CAACCTCGACTTGGCCTCCCTTCTAGAGATTACTATGAATGCACTGGAATCTATAAAGAG
GCTTGTACAGCATATGTGGATTTTATGATTTCTGTGGCCAGATTGATTCGTCAGGAAGAA
AGATTGCCCATCGATGAAAACCAGCTTGCTTTGGAAATGAATAAAGTTATGGAATTGGAA
AAAGAAATTGCCAATGCTACGGCTAAACCTGAAGATCGAAATGATCCAATGCTTCTGTAT
AACAAGATGACATTGGCCCAGATCCAAAATAACTTTTCACTAGAGATCAATGGGAAGCCA
TTCAGCTGGTTGAATTTCACAAATGAAATCATGTCAACTGTGAATATTAGTATTACAAAT
GAGGAAGATGTGGTTGTTTATGCTCCAGAATATTTAACCAAACTTAAGCCCATTCTTACC
AAATATTCTGCCAGAGATCTTCAAAATTTAATGTCCTGGAGATTCATAATGGATCTTGTA
AGCAGCCTCAGCCGAACCTACAAGGAGTCCAGAAATGCTTTCCGCAAGGCCCTTTATGGT
ACAACCTCAGAAACAGCAACTTGGAGACGTTGTGCAAACTATGTCAATGGGAATATGGAA
AATGCTGTGGGGAGGCTTTATGTGGAAGCAGCATTTGCTGGAGAGAGTAAACATGTGGTC
GAGGATTTGATTGCACAGATCCGAGAAGTTTTTATTCAGACTTTAGATGACCTCACTTGG
ATGGATGCCGAGACAAAAAAGAGAGCTGAAGAAAAGGCCTTAGCAATTAAAGAAAGGATC
GGCTATCCTGATGACATTGTTTCAAATGATAACAAACTGAATAATGAGTACCTCGAGTTG
AACTACAAAGAAGATGAATACTTCGAGAACATAATTCAAAATTTGAAATTCAGCCAAAGT
AAACAACTGAAGAAGCTCCGAGAAAAGGTGGACAAAGATGAGTGGATAAGTGGAGCAGCT
GTAGTCAATGCATTTTACTCTTCAGGAAGAAATCAGATAGTCTTCCCAGCCGGCATTCTG
CAGCCCCCCTTCTTTAGTGCCCAGCAGTCCAACTCATTGAACTATGGGGGCATCGGCATG
GTCATAGGACACGAAATCACCCATGGCTTCGATGACAATGGCAGAAACTTTAACAAAGAT
GGAGACCTCGTTGACTGGTGGACTCAACAGTCTGCAAGTAACTTTAAGGAGCAATCCCAG
TGCATGGTGTATCAGTATGGAAACTTTTCCTGGGACCTGGCAGGTGGACAGCACCTTAAT
GGAATTAATACACTGGGAGAAAACATTGCTGATAATGGAGGTCTTGGTCAAGCATACAGA
GCCTATCAGAATTATATTAAAAAGAATGGCGAAGAAAAATTACTTCCTGGACTTGACCTA
AATCACAAACAACTATTTTTCTTGAACTTTGCACAGGTGTGGTGTGGAACCTATAGGCCA
GAGTATGCGGTTAACTCCATTAAAACAGATGTGCACAGTCCAGGCAATTTCAGGATTATT
GGGACTTTGCAGAACTCTGCAGAGTTTTCAGAAGCCTTTCACTGCCGCAAGAATTCATAC
ATGAATCCAGAAAAGAAGTGCCGGGTTTGGTGA

Clone variation with respect to NM_000902.3
5' Read Nucleotide Sequence
>OriGene 5' read for NM_000902 unedited
GGTGTTCGAATTTTGTAATACGACTCACTATAGGGCGGCCCGCGAATTCGCACGAGCAGC
AGCCTCCAACTTCTCCCGAATCCCACTGATTTTAGGTGATGGGCAAGTCAGAAAGTCAGA
TGGATATAACTGATATCAACACTCCAAAGCCAAAGAAGAAACAGCGATGGACTCCACTGG
AGATCAGCCTCTCGGTCCTTGTCCTGCTCCTCACCATCATAGCTGTGACAATGATCGCAC
TCTATGCAACCTACGATGATGGTATTTGCAAGTCATCAGACTGCATAAAATCAGCTGCTC
GACTGATCCAAAACATGGATGCCACCACTGAGCCTTGTACAGACTTTTTCAAATATGCTT
GCGGAGGCTGGTTGAAACGTAATGTCATTCCCGAGACCAGCTCCCGTTACGGCAACTTTG
ACATTTTAAGAGATGAACTAGAAGTCGTTTTGAAAGATGTCCTTCAAGAACCCAAAACTG
AAGATATAGTAGCAGTGCAGAAAGCAAAAGCATTGTACAGGTCTTGTATAAATGAATCTG
CTATTGATAGCAGAGGTGGAGAACCTCTACTCAAACTGTTACCAGACATATATGGGTGGC
CAGTAGCAACAGAAAACTGGGAGCAAAAATATGGTGCTTCTTGGACAGCTGAAAAAGCTA
TTGCACAACTGAATTCTAAATATGGGAAAAAAGTCCTTATTAATTTGTTTGTTGGCACTG
ATGATAAGAATTCTGTGAATCATGTAATTCATATTGACCAACCTCGACTTGGCCTCCCTT
CTAGAGATACTATGAATGCACTGGAAATCTATAAGAGGCTTGTACAGCATATGTGGATTT
TATGAATTCTGTGGCCAGAATGATTCGTCAGGAAGAAAGATTGCCATCGATGAAACCAGC
TTGCTTTGGAATGAATAAAGTATGGAA
3' Read Nucleotide Sequence
>OriGene 3' read for NM_000902 unedited
CCCCATTTGTTGCCCGCCGGCCGCATTCTACATCGCGTTTTTTTTTTTTTTTTTTACCAT
CACCAACGTTTGTTTTTATTTTGCATTTTTGAGACTGGAAACTGATAAACATTTCTGATT
AGGAAACTTTGACAATACAGTCTTTCTTCTTTCCAAAATAACAAATGTCTTTTAGTTTGA
GAGCTCCAGCTGTGGCAACAAGAATGATAAATGCTTTTGAGAAAATTAGCACGGTTTTGC
CCCACTTAAATGGTTAACAGTAACAGTAGTACAGACCCAAACTTGACAAAACTTTGGCAC
TTGTTATTTGGACTAAAATTTTGTCTCACTCCATTCACACACGTCCATGGGAGTTTTCAG
TTTATCCTGCCCACTGATTCATACCTGGCAGACCGATTCCCTGGGACGGCAGCATTGGAC
ACCTACTCCTTCTCTCGCTGACAACCACCAATATACTCTAATTCTCTTTTTCTAGGCGAG
AGACGCCCCGGCCCACCGGAAAATTGCACACCCCCCAGGCCCCCCTCGCCCGAGCGCCAC
ACCCCGCCCCCAACAGGCACTTTTTCCTTGCCCCGCTGCCCTTCCAATCCTTCCACTTCG
CCCCTCCCTCCCACCAACACCGCTCCTCGCAGCTATCTTCCTCCCCACCCCCCCCTGCCC
CCCGCCCCTTCTCCCCACCTCTCTCGCCACTTTCCTACCTCTCCAACCATTTTTGTCTAT
AAAAATTTATCAATTCCCTCTCTTCCCCCCATACCCTCCTCCCTCTCACCCCTCGTCTCT
ACTGCCCGTCACACACCCCAAACGCCCCCCCTGCCCGTCCCAACCCACCACGACGCCGCC
CCCCCGCCTCACCCATCACACATAGTCATCCCACCCCATCCCCCCCTCAACTCCCTCCCC
ACTCCCTCCCTCTCCGCCTCCTCCCCACCCGCTCCCC
Restriction Sites NotI-NotI |
ACCN NM_000902
Insert Size 5850 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000902.2, NP_000893.1
RefSeq Size 5595 bp
RefSeq ORF 2253 bp
Locus ID 4311
UniProt ID P08473
Cytogenetics 3q25.2
Domains Peptidase_M13
Protein Families Druggable Genome, Protease, Transmembrane
Protein Pathways Alzheimer's disease, Hematopoietic cell lineage, Renin-angiotensin system
Summary The protein encoded by this gene is a type II transmembrane glycoprotein and a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). The encoded protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. provided by RefSeq, Aug 2017
Transcript Variant: This variant (1) contains an alternate 5' UTR exon compared to variant 2b. Variants 1, 1bis, 2a, 2b, 3, and 4 all encode the same isoform (a).
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Documents
Resources
"NM_000902" in other vectors (6)
SKU Description Size Price
RC223013 MME (Myc-DDK-tagged)-Human membrane metallo-endopeptidase (MME), transcript variant 1 10 ug
$687.00
RC223013L1 Lenti ORF clone of Human membrane metallo-endopeptidase (MME), transcript variant 1, Myc-DDK-tagged 10 ug
$987.00
RC223013L2 Lenti ORF clone of Human membrane metallo-endopeptidase (MME), transcript variant 1, mGFP tagged 10 ug
$987.00
RC223013L3 Lenti ORF clone of Human membrane metallo-endopeptidase (MME), transcript variant 1, Myc-DDK-tagged 10 ug
$987.00
RC223013L4 Lenti ORF clone of Human membrane metallo-endopeptidase (MME), transcript variant 1, mGFP tagged 10 ug
$987.00
RG223013 MME (tGFP-tagged) - Human membrane metallo-endopeptidase (MME), transcript variant 1 10 ug
$789.00 $887.00

file-alt Citations

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