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WNT5B (NM_030775) Human Untagged Clone

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SKU
SC109435
WNT5B (untagged)-Human wingless-type MMTV integration site family, member 5B (WNT5B), transcript variant 2
  $457.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol WNT5B
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_030775 edited
GAATTCGGCACGAGGATTTCTTTGGATGTTCTGCAGCAAGGACAGGTATGCTTTACAACA
GCCGAAGTGGCCTCCCGGCTGCCGAACGGAGGAACGCCGCAAGCTCCGCTCTTGAAATTA
CTTGTTTTCATTTCTCTTTGTGGTTTCTCAGCTCATTATTTCTTTGGAAATTAGGTCCTG
TGCAGGGTTCCCACAGTTTGGGGTAAGAGACAGAAGTCCTAGGGTGGGAAGATGAGCAGT
GGGAGGCGGAGGCTGGAAAGAGGCCGAGCTTCTTTGTGGGGAACACGCAGCACGTAAGCA
TCAGTGCAACTTTCTCCGCCTCACCCCGGCTCCTGGTCTGCCCTTATCCGCTGAGTTTCC
ACACTGACTCTCCATTTCTGTTTTCTCCAGGGAACCCTACTCTGGAAACTGTCAGTCCCA
GGGCACTGGGGAGGGCTGAGGCCGACCATGCCCAGCCTGCTGCTGCTGTTCACGGCTGCT
CTGCTGTCCAGCTGGGCTCAGCTTCTGACAGACGCCAACTCCTGGTGGTCATTAGCTTTG
AACCCGGTGCAGAGACCCGAGATGTTTATCATCGGTGCCCAGCCCGTGTGCAGTCAGCTT
CCCGGGCTCTCCCCTGGCCAGAGGAAGCTGTGCCAATTGTACCAGGAGCACATGGCCTAC
ATAGGGGAGGGAGCCAAGACTGGCATCAAGGAATGCCAGCACCAGTTCCGGCAGCGGCGG
TGGAATTGCAGCACAGCGGACAACGCATCTGTCTTTGGGAGAGTCATGCAGATAGGCAGC
CGAGAGACCGCCTTCACCCACGCGGTGAGCGCCGCGGGCGTGGTCAACGCCATCAGCCGG
GCCTGCCGCGAGGGCGAGCTCTCCACCTGCGGCTGCAGCCGGACGGCGCGGCCCAAGGAC
CTGCCCCGGGACTGGCTGTGGGGCGGCTGTGGGGACAACGTGGAGTACGGCTACCGCTTC
GCCAAGGAGTTTGTGGATGCCCGGGAGCGAGAGAAGAACTTTGCCAAAGGATCAGAGGAG
CAGGGCCGGGTGCTCATGAACCTGCAAAACAACGAGGCCGGTCGCAGGGCTGTGTATAAG
ATGGCAGACGTAGCCTGCAAATGCCACGGCGTCTCGGGGTCCTGCAGCCTCAAGACCTGC
TGGCTGCAGCTGGCCGAGTTCCGCAAGGTCGGGGACCGGCTGAAGGAGAAGTACGACAGC
GCGGCCGCCATGCGCGTCACCCGCAAGGGCCGGCTGGAGCTGGTCAACAGCCGCTTCACC
CAGCCCACCCCGGAGGACCTGGTCTATGTGGACCCCAGCCCCGACTACTGCCTGCGCAAC
GAGAGCACGGGCTCCCTGGGCACGCAGGGCCGCCTCTGCAACAAGACCTCGGAGGGCATG
GATGGCTGTGAGCTCATGTGCTGCGGGCGTGGCTACAACCAGTTCAAGAGCGTGCAGGTG
GAGCGCTGCCACTGCAAGTTCCACTGGTGCTGCTTCGTCAGGTGTAAGAAGTGCACGGAG
ATCGTGGACCAGTACATCTGTAAATAGCCCGGAGGGCCTGCTCCCGGCCCCCCTGCACTC
TGCCTCACAAAGGTCTATATTATATAAATCTATATAAATCTATTTTATATTTGTATAAGT
AAATGGGTGGGTGCTATACAATGGAAAGATGAAAATGGAAAGGAAGAGCTTATTTAAGAG
ACGCTGGAGATCTCTGAGGAGTGGACTTTGCTGGTTCTCTCCTCTTGGTGGGTGGGAGAC
AGGGCTTTTTCTCTCCCTCTGGTGAGGACTCTCAGGATGTAGGGACTTGGAAATATTTAC
TGTCTGTCCACCACGGCCTGGAGGAGGGAGGTTGTGGTTGGATGGAGGAGATGATCTTGT
CTGGAAGTCTAGAGTCTTTGTTGGTTAGAGGACTGCCTGTGATCCTGGCCACTAGGCCAA
GAGGCCCTATGAAGGTGGCGGGAACTCAGCTTCAACCTCGATGTCTTCAGGGTCTTGTCC
AGAATGTAGATGGGTTCCGTAAGAGGCCTGGTGCTCTCTTACTCTTTCATCCACGTGCAC
TTGTGCGGCATCTGCAGTTTACAGGAACGGCTCCTTCCCTAAAATGAGAAGTCCAAGGTC
ATCTCTGGCCCAGTGACCACAGAGAGATCTGCACCTCCCGGACTTCAGGCCTGCCTTTCC
AGCGAGAATTCTTCATCCTCCACGGTTCACTAGCTCCTACCTGAAGAGGAAAGGGGGCCA
TTTGACCTGACATGTCAGGAAAGCCCTAAACTGAATGTTTGCGCCTGGGCTGCAGAAGCC
AGGGTGCATGACCAGGCTGCGTGGACGTTATACTGTCTTCCCCCACCCCCGGGGAGGGGA
AGCTTGAGCTGCTGCTGTCACTCCTCCACCGAGGGAGGCCTCACAAACCACAGGACGCTG
CAACGGGTCAGGCTGGCGGGCCCGGCGTGCTCATCATCTCTGCCCCAGGTGTACGGTTTC
TCTCTGACATTAAATGCCCTTCATGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCGAC
5' Read Nucleotide Sequence
>OriGene 5' read for NM_030775 unedited
CATATTATGNATACGACTCACTATAGGGCGGCCCGCGAATCGGCACGAGGATTTCTTTGG
ATGTTCTGCAGCAAGGACAGGTATGCTTTACAACAGCCGAAGTGGCCTCCCGGCTGCCGA
ACGGAGGAACGCCGCAAGCTCCGCTCTTGAAATTACTTGTTTTCATTTCTCTTTGTGGTT
TCTCAGCTCATTATTTCTTTGGAAATTAGGTCCTGTGCAGGGTTCCCACAGTTTGGGGTA
AGAGACAGAAGTCCTAGGGTGGGAAGATGAGCAGTGGGAGGCGGAGGCTGGAAAGAGGCC
GAGCTTCTTTGTGGGGAACACGCAGCACGTAAGCATCAGTGCAACTTTCTCCGCCTCACC
CCGGCTCCTGGTCTGCCCTTATCCGCTGAGTTTCCACACTGACTCTCCATTTCTGTTTTC
TCCAGGGAACCCTACTCTGGAAACTGTCAGTCCCAGGGCACTGGGGAGGGCTGAGGCCGA
CCATGCCCAGCCTGCTGCTGCTGTTCACGGCTGCTCTGCTGTCCAGCTGGGCTCAGCTTC
TGACAGACGCCAACTCCTGGTGGTCATTAGCTTTGAACCCGGTGCAGAGACCCGAGATGT
TTATCATCGGTGCCCAGCCCGTGTGCAGTCAGCTTCCCGGGCTCTCCCCTGGCCAGAGGA
AGCTGTGCCAATTGTACCAGGAGCACATGGCCTACATAGGGGAGGGAGCCAAGACTGGCA
TCAAGGAATGCCAGCACCAGTTCCGGCAGCGGCGGTGGGATTGCAGCACAGCGGACAACG
CATCTGTCTTTGGGAGAGTCATGCAGATAGGCAGCCGAGAGACCGNCTTCACCCACGCCG
TGAGCGCCGCGGGCGTGGTCAACGCCATCAGCCGGGCCTGCCGCGAGGGCGAGCTCTNCA
CCTGCGNTGCAGCCGGACGGNGCGGCCCAGGACCTGCCCGGACTGGCTGTGNGCCGCTGT
GGG
3' Read Nucleotide Sequence
>OriGene 3' read for NM_030775 unedited
NNTTTTTTNNGGGGCATTTATGTNCAGTAGGNAACCGTACACCTGGGGCAGNANAGATGA
GCACGCCGGGCCCGCCAGCCTGACCCGTTGCAGCGTCCTGTGGTTTGTGAGGCCTCCCTC
GGTGGAGGAGTGACAGCAGCAGCTCAAGCTTCCCCTCCCCGGGGGTGGGGGAAGACAGTA
TAACGTCCACGCAGCCTGGTCATGCACCCTGGCTTCTGCAGCCCAGGCGCAAACATTCAG
TTTAGGGCTTTCCTGACATGTCAGGTCAAATGGCCCCCTTTCCTCTTCAGGTAGGAGCTA
GTGAACCGTGGAGGATGAAGAATTCTCGCTGGAAAGGCAGGCCTGAAGTCCGGGAGGTGC
AGATCTCTCTGTGGTCACTGGGCCAGAGATGACCTTGGACTTCTCATTTTAGGGAAGGAG
CCGTTCCTGTAAACTGCAGATGCCGCACAAGTGCACGTGGATGAAAGAGTAAGAGAGCAC
CAGGCCTCTTACGGAACCCATCTACATTCTGGACAAGACCCTGAAGACATCGAGGTTGAA
GCTGAGTTCCCGCCACCTTCATAGGGCCTCTTGGCCTAGTGGCCAGGATCACAGGCAGTC
CTCTAACCAACAAAGACTCTAGACTTCCAGACAAGATCATCTCCTCCATCCAACCACAAC
CTCCCTCCTNCAGGCCGTGGTGGACAGACAGTAAATATTTCCAAGTCCCTACATCCTGAG
AGTCCTCACCANGAGGAGAGAAAAAGCCCTGTCTCCCACCCACCAAGAGGAGAGAACCAG
CAAAGTCCACTCCTCAGAGATCTCCANCGTCTCTTAAATAGCTCTTCCTTTCCATTNTCA
TCTTTCCATTGTATAGCACCCACCCCTTTACTTATACAAATATAAATAGATTTATATAGA
TTTATATATATAGACCCTTG
Restriction Sites NotI-NotI |
ACCN NM_030775
Insert Size 2610 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_030775.2, NP_110402.2
RefSeq Size 2184 bp
RefSeq ORF 1080 bp
Locus ID 81029
UniProt ID Q9H1J7
Cytogenetics 12p13.33
Domains wnt
Protein Families Secreted Protein
Protein Pathways Basal cell carcinoma, Hedgehog signaling pathway, Melanogenesis, Pathways in cancer, Wnt signaling pathway
Summary The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 94% and 80% amino acid identity to the mouse Wnt5b protein and the human WNT5A protein, respectively. Alternative splicing of this gene generates 2 transcript variants. provided by RefSeq, Jul 2008
Transcript Variant: This variant (2) differs from variant 1 in the 5' UTR, which results from an alternate exon. Both variants encode the same protein.
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Documents
Resources
"NM_030775" in other vectors (6)
SKU Description Size Price
RC200847 WNT5B (Myc-DDK-tagged)-Human wingless-type MMTV integration site family, member 5B (WNT5B), transcript variant 2 10 ug
$457.00
RC200847L1 Lenti ORF clone of Human wingless-type MMTV integration site family, member 5B (WNT5B), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC200847L2 Lenti ORF clone of Human wingless-type MMTV integration site family, member 5B (WNT5B), transcript variant 2, mGFP tagged 10 ug
$757.00
RC200847L3 Lenti ORF clone of Human wingless-type MMTV integration site family, member 5B (WNT5B), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC200847L4 Lenti ORF clone of Human wingless-type MMTV integration site family, member 5B (WNT5B), transcript variant 2, mGFP tagged 10 ug
$757.00
RG200847 WNT5B (tGFP-tagged) - Human wingless-type MMTV integration site family, member 5B (WNT5B), transcript variant 2 10 ug
$489.00 $657.00

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