ITM2C (NM_030926) Human Untagged Clone

SKU SC109191

ITM2C (untagged)-Human integral membrane protein 2C (ITM2C), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: ITM2C
• Synonyms: BRI3; BRICD2C; E25; E25C; ITM3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109191 sequence for NM_030926 edited (data generated by NextGen Sequencing); ATGGTGAAGATTAGCTTCCAGCCCGCCGTGGCTGGCATCAAGGGCGACAAGGCTGACAAG; GCGTCGGCGTCGGCCCCTGCGCCGGCCTCGGCCACCGAGATCCTGCTGACGCCGGCTAGG; GAGGAGCAGCCCCCACAACATCGATCCAAGAGGGGGAGCTCAGTGGGCGGCGTGTGCTAC; CTGTCGATGGGCATGGTCGTGCTGCTCATGGGCCTCGTGTTCGCCTCTGTCTACATCTAC; AGATACTTCTTTCTTGCACAGCTGGCCCGAGATAACTTCTTCCGCTGTGGTGTGCTGTAT; GAGGACTCCCTGTCCTCCCAGGTCCGGACTCAGATGGAGCTGGAAGAGGATGTGAAAATC; TACCTCGACGAGAACTACGAGCGCATCAACGTGCCTGTGCCCCAGTTTGGCGGCGGTGAC; CCTGCAGACATCATCCATGACTTCCAGCGGGGTCTGACTGCGTACCATGATATCTCCCTG; GACAAGTGCTATGTCATCGAACTCAACACCACCATTGTGCTGCCCCCTCGCAACTTCTGG; GAGCTCCTCATGAACGTGAAGAGGGGGACCTACCTGCCGCAGACGTACATCATCCAGGAG; GAGATGGTGGTCACGGAGCATGTCAGTGACAAGGAGGCCCTGGGGTCCTTCATCTACCAC; CTGTGCAACGGGAAAGACACCTACCGGCTCCGGCGCCGGGCAACGCGGAGGCGGATCAAC; AAGCGTGGGGCCAAGAACTGCAATGCCATCCGCCACTTCGAGAACACCTTCGTGGTGGAG; ACGCTCATCTGCGGGGTGGTGTGA; ; Clone variation with respect to NM_030926.4; 157 g=>a;252 c=>t;258 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_030926 unedited; AAGTTCAAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGAGGCTGCA; CCGGCAGAGGCTGCGGGGCGGACGCGCGGGCCGGCGCAGCCATGGTGAAGATTAGCTTCC; AGCCCGCCGTGGCTGGCATCAAGGGCGACAAGGCTGACAAGGCGTCGGCGTCGGCCCCTG; CGCCGGCCTCGGCCACCGAGATCCTGCTGACGCCGGCTAGGGAGGAGCAGCCCCCACAAC; ATCGATCCAAGAGGGGGAGCTCAGTGGGCGGCGTGTGCTACCTGTCGATGGGCATGGTCG; TGCTGCTCATGGGCCTCGTGTTCGCCTCTGTCTACATCTACAGATACTTCTTTCTTGCAC; AGCTGGCCCGAGATAACTTCTTCCGCTGTGGTGTGCTGTATGAGGACTCCCTGTCCTCCC; AGGTCCGGACTCAGATGGAGCTGGAAGAGGATGTGAAAATCTACCTCGACGAGAACTACG; AGCGCATCAACGTGCCTGTGCCCCAGTTTGGCGGCGGTGACCCTGCAGACATCATCCATG; ACTTCCAGCGGGGTCTGACTGCGTACCATGATATCTCCCTGGACAAGTGCTATGTCATCG; AACTCAACACCACCATTGTGCTGCCCCCTCGCAACTTCTGGGAGCTCCTCATGAACGTGA; AGAGGGGGACCTACCTGCCGCAGACGTACATCATCCAGGAGGAGATGGTGGTCACGGAGC; ATGTCAGTGACAAGGAGGCCCTGGGGTCCTTCATCTACCACCTGTGCAACGGGAAAGACA; CCTACCGGCTCCGGCGCCGGGCAACGCGNNNAGCGGATCAACAGCGTGGGGCCCAAGAAC; TGCATGCCATCCGCCACTTCGAGAACACCTTNCGTGTGNAGACGCTCATCTGCNGNGTGG; GTGTGAGGCCCTCCTCCCCCANAAC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_030926 unedited; GGCCGCAATCTANAGTCGAGNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT; TTTTTTTTTTTTTTTTTCAAAAAGAAAAAATATTTTTTTTGTTTTATAAACCCTTTTGAA; AAAAAATTTATTTGAAAAAATTTCCAAAGCTTTTAAAAGTTTCTTCCCCAAAAACTGATT; GAAGGGGTTACACCCCTCCTTTCCAAGGTTAAGGGTTGAAAACGGTTACCTTTTCGAATG; ATCAGTAAAAACTTCCAAAAGTGAAAAGGAAGGGGAAAAAGGGGCTTTCCCCTAAGCTGG; GGGGGAGGGAATTTCAAAACAAAGGAGGCGGGGGGGACAAGCCCCAGGGGGCTCCCCCTT; TCCCTTTGGGTTATTTTTAAAAACAGTTCCAAGCTTGGAAAAAGCAATGACCTCCCCCTA; CTAAGAAAAACCCACGGTTCGCCCCCCGGGGACGTGACTGAACAGGGACCTTGCTTGCCC; CGTTCCTAAGCCGCCCTTCCCACTGCTTTGACTGAGGGGACCCTGTTTGTCCTCCTGGGG; GGCAGAACTGGGGGCCCTACCTCCTTGTTTTTTTGGTCCCACTCAAAGAACAGTTGGGCT; TGACGGACTCATCCCCACTCCCAAAACTCAAAGCTCCTGTTGGGGGGCTAAAGGCACTGA; CAAATTCCCTGGGACACCCCCAGGNACCCCCTTCTTTTGTGCTCTGGNGGGCCCCTCCTT; GGGCAATTGCCCCATCCTTATTAAACCCCAGGTTTAGAAATGGGACGGGGAAAAAGTGCC; CCTCCAAAAAAACTTTGAGGACTTTTCTAATGGCAAAACTTTTGAGCCATGCCGGCCGNC; CCAAAACCATTCCTTGGCCAGGGCTTAATGGCGGGCTCCCCACGGTCTTCCCTGGTTGCC; GGGCATTGTGGCCCAGCTTTGCGGCCCATTTAAAACGGGGAACCAAAAACTTTGTACAAT; CCTT;
• Restriction Sites: NotI-NotI
• ACCN: NM_030926
• Insert Size: 2140 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_030926.4, NP_112188.1
• RefSeq Size: 2123 bp
• RefSeq ORF: 804 bp
• Locus ID: 81618
• UniProt ID: Q9NQX7
• Cytogenetics: 2q37.1
• Domains: BRICHOS
• Protein Families: Transmembrane
• Summary: Negative regulator of amyloid-beta peptide production. May inhibit the processing of APP by blocking its access to alpha- and beta-secretase. Binding to the beta-secretase-cleaved APP C-terminal fragment is negligible, suggesting that ITM2C is a poor gamma-secretase cleavage inhibitor. May play a role in TNF-induced cell death and neuronal differentiation (By similarity).[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) encodes the longest isoform (1). Both variants 1 and 5 encode the same isoform.