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EEF1D (NM_001960) Human Untagged Clone

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SKU
SC109188
EEF1D (untagged)-Human eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein) (EEF1D), transcript variant 2
  $300.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol EEF1D
Synonyms EF-1D; EF1D; FP1047
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC109188 sequence for NM_001960 edited (data generated by NextGen Sequencing)
ATGGCTACAAACTTCCTAGCACATGAGAAGATCTGGTTCGACAAGTTCAAATATGACGAC
GCAGAAAGGAGATTCTACGAGCAGATGAACGGGCCTGTGGCAGGKGCCTCCCGCCAGGAG
AACGGCGCCAGCGTGATCCTCCGTGACATTGCGAGAGCCAGAGAGAACATCCAGAAATCC
CTGGCTGGAAGCTCAGGCCCCGGGGCCTCCAGCGGCACCAGCGGAGACCACGGTGAGCTC
GTCGTCCGGATTGCCAGTCTGGAAGTGGAGAACCAGAGTCTGCGTGGCGTGGTACAGGAG
CTGCAGCAGGCCATCTCCAAGCTGGAGGCCCGGCTGAACGTGCTGGAGAAGAGCTCGCCT
GGCCACCGGGCCACGGCCCCACAGACCCAGCACGTATCTCCCATGCGCCAAGTGGAGCCC
CCAGCCAAGAAGCCAGCCACACCAGCAGAGGATGACGAGGATGATGACATTGACCTGTTT
GGCAGTGACAATGAGGAGGAGGACAAGGAGGCGGCACAGCTGCGGGAGGAGCGGCTACGG
CAGTACGCGGAGAAGAAGGCCAAGAAGCCTGCACTGGTGGCCAAGTCCTCCATCCTGCTG
GATGTCAAGCCTTGGGATGATGAGACGGACATGGCCCAGCTGGAGGCCTGTGTGCGCTCT
ATCCAGCTGGACGGGCTGGTCTGGGGGGCTTCCAAGCTGGTGCCCGTGGGCTACGGTATC
CGGAAGCTACAGATTCAGTGTGTGGTGGAGGACGACAAGGTGGGGACAGACTTGCTGGAG
GAGGAGATCACCAAGTTTGAGGAGCACGTGCAGAGTGTCGATATCGCAGCTTTCAACAAG
ATCTGA

Clone variation with respect to NM_001960.4
105 t=>k
5' Read Nucleotide Sequence
>OriGene 5' read for NM_001960 unedited
NGGGCACCATTTGTATACGACTCCTATAGGCGGCCCGCGAATTCGCACGAGGGCCGAGGG
TGCCCGGCGGGGCCGCAGAGACCGGCGGGGCCGCAACATCTTAGGGAACAAGCGGGCCGG
GCTGCGACGGGCCGATGGGGAGGCCCCCTCTGCCTTGCCCTACTGTTACTTCCTGCAGAA
GGATGCAGAGGCCCCCTGGCTCAGCAAGCCTGCCTACGACAGCGCCGAGTGCCGCCACCA
CGCTGCCGAGGCCCTGCGTGTGGCCTGGTGCCTCGAAGCTGCCTCCCTGTCTCACCGACC
CGGTCCTCGGTCTGGCCTGTCCGTGTCCAGCCTGAGACCCAACAGAAAAATGGCTACAAA
CTTCCTAGCACATGAGAAGATCTGGTTCGACAAGTTCAAATATGACGACGCAGAAAGGAG
ATTCTACGAGCAGATGAACGGGCCTGTGGCAGGTGCCTCCCGCCAGGAGAACGGCGCCAG
CGTGATCCTCCGTGACATTGCGAGAGCCAGAGAGAACATCCAGAAATCCCTGGCTGGAAG
CTCAGGCCCCGGGGCCTCCAGCGGCACCAGCGGAGACCACGGTGAGCTCGTCGTCCGGAT
TGCCAGTCTGGAAGTGGAGAACCAGAGTCTGCGTGGCGTGGTACAGGAGCTGCAGCAGGC
CATCTCCAAGCTGGAGGCCCCGCTGAACGTGCTGGAGAAGAGCTCGCCTGGCCACCGGGC
CACGGCCCCACAGACCCCGCACGTATCTCCCATGCGCCCAGTGGAGCCCCCAGCCAAGAA
GCCAGCCACACCANCANAGNATGACGAAGATGATGACCATTGACCTGTTTGCCAGTGACC
ATGGAGGAGGAGGACCAGGAAGCGGCCCAGCTGCCGGAAGAAAGCGCTACCGCCAGTACC
CGAAGAAA
3' Read Nucleotide Sequence
>OriGene 3' read for NM_001960 unedited
GACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTGCCGGGCTCAGTCTTTAATCGGGGGAGGGCCTTACGCACGCGCGCACGTACACACAC
TTAGGCTTCAAATCTTGGTGAAAGCTGCGATATCGACACTTTGCACGTGCTCCTCAAACT
TGGGGATCTCCTCCTCCAGCAAGTCTGTCCCCACCTTGGCGTCCTCCACCACACACTGAA
TCTGTAGCTTCCGGATACCGGAACCCACGGGCACCAGCTTGGAAGCCCCCCAAACCAACC
CGTCCAGCTGGATAAAGCGCACACAGGCCTCCAGCTGGGCCATGTCCGTCTCATCATCCC
AAGGGTTGACATCCAGCAGGATGGAGGACTTGGCCACCAGTGCAGGCTTTTTGGCCTTTT
TCTCCGCGTACTGGCGTAACCGCTCCTCCCGCAGCTGGGCCGCCTCCTTGGCCTCCTCCT
CATTGGCACTGGCAAACAGGGCAATGGCATCATCCTCGGCATCCTCTGGTGGGGGGGCTG
GCTTCTTGGCTGGGGGCTCCACTTGGCGCATGGGAGATACCTGCTGGGTCTGTGGGGCCG
TGGCCCGGTGGCCAAGCGAGCTCTTCTCCAGCACGTTCAGCCGGGCCTTCAGCTTGGAGA
TGGGCTGCTGCAACTTCTGTACCACGCCACGCAGACTCTGGTTCTTCACTTCCAGACTGG
CAATCCCGACGACGAGCTCCCCGGGTCTCCCCTGGTGCCGCTGGAGGGCCCGGGGCCTCG
AGCCTCCAACCAAGGATTTCTGGGAAGGTCTCTTCTGCCTCTCGCCATGGTCCCGGAGGA
TCAAGCTTGGCGCCCGTCTCCTGGCGGGAAGCCACCTGGCCAAGCCCGGTTATTTGGTTC
GGAAAACTCCTT
Restriction Sites NotI-NotI |
ACCN NM_001960
Insert Size 1360 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001960.2, NP_001951.2
RefSeq Size 994 bp
RefSeq ORF 846 bp
Locus ID 1936
UniProt ID P29692
Cytogenetics 8q24.3
Domains EF1BD
Summary This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit, delta, functions as guanine nucleotide exchange factor. It is reported that following HIV-1 infection, this subunit interacts with HIV-1 Tat. This interaction results in repression of translation of host cell proteins and enhanced translation of viral proteins. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. Related pseudogenes have been defined on chromosomes 1, 6, 7, 9, 11, 13, 17, 19.provided by RefSeq, Aug 2010
Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Variants 2, 5, 6, and 9 encode the same isoform (2).
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Documents
Resources
"NM_001960" in other vectors (5)
SKU Description Size Price
RC203202 EEF1D (Myc-DDK-tagged)-Human eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein) (EEF1D), transcript variant 2 10 ug
$603.00
RC203202L3 Lenti ORF clone of Human eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein) (EEF1D), transcript variant 2, Myc-DDK-tagged 10 ug
$903.00
RC203202L4 Lenti ORF clone of Human eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein) (EEF1D), transcript variant 2, mGFP tagged 10 ug
$903.00
RG203202 EEF1D (tGFP-tagged) - Human eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein) (EEF1D), transcript variant 2 10 ug
$489.00 $803.00
SC320760 EEF1D (untagged)-Human eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein) (EEF1D), transcript variant 2 10 ug
$300.00

file-alt Citations

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