ATF3 (NM_001674) Human Untagged Clone

SKU SC108959

ATF3 (untagged)-Human activating transcription factor 3 (ATF3), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: ATF3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001674 edited; GAATTCGGCACGAGGGCGCACTGCACAGCTCTCTTCTCTCGCCGCCGCCCGAGCGCACCC; TTCAGCCCGCGCGCCGGCCGTGAGTCCTCGGTGCTCGCCCGCCGGCCAGACAAACAGCCC; GCCCGACCCCGTCCCGACCCTGGCCGCCCCGAGCGGAGCCTGGAGCAAAATGATGCTTCA; ACACCCAGGCCAGGTCTCTGCCTCGGAAGTGAGTGCTTCTGCCATCGTCCCCTGCCTGTC; CCCTCCTGGGTCACTGGTGTTTGAGGATTTTGCTAACCTGACGCCCTTTGTCAAGGAAGA; GCTGAGGTTTGCCATCCAGAACAAGCACCTCTGCCACCGGATGTCCTCTGCGCTGGAATC; AGTCACTGTCAGCGACAGACCCCTCGGGGTGTCCATCACAAAAGCCGAGGTAGCCCCTGA; AGAAGATGAAAGGAAAAAGAGGCGACGAGAAAGAAATAAGATTGCAGCTGCAAAGTGCCG; AAACAAGAAGAAGGAGAAGACGGAGTGCCTGCAGAAAGAGTCGGAGAAGCTGGAAAGTGT; GAATGCTGAACTGAAGGCTCAGATTGAGGAGCTCAAGAACGAGAAGCAGCATTTGATATA; CATGCTCAACCTTCATCGGCCCACGTGTATTGTCCGGGCTCAGAATGGGAGGACTCCAGA; AGATGAGAGAAACCTCTTTATCCAACAGATAAAAGAAGGAACATTGCAGAGCTAAGCAGT; CGTGGTATGGGGGCGACTGGGGAGTCCTCATTGAATCCTCATTTTATACCCAAAACCCTG; AAGCCATTGGAGAGCTGTCTTCCTGTGTACCTCTAGAATCCCAGCAGCAGAGAACCATCA; AGGCGGGAGGGCCTGCAGTGATTCAGCAGGCCCTTCCCATTCTGCCCCAGAGTGGGTCTT; GGACCAGGGCAAGTGCATCTTTGCCTCAACTCCAGGATTTAGGCCTTAACACACTGGCCA; TTCTTATGTTCCAGATGGCCCCCAGCTGGTGTCCTGCCCGCCTTTCATCTGGATTCTACA; AAAAACCAGGATGCCCACCGTTAGGATTCAGGCAGCAGTGTCTGTACCTCGGGTGGGAGG; GATGGGGCCATCTCCTTCACCGTGGCTACCATTGTCACTCGTAGGGGATGTGGAGTGAGA; ACAGCATTTAGTGAAGTTGTGCAACGGCCAGGGTTGTGCTTTCTAGCAAATATGCTGTTA; TGTCCAGAAATTGTGTGTGCAAGAAAACTAGGCAATGTACTCTTCCGATGTTTGTGTCAC; ACAACACTGATGTGACTTTTATATGCTTTTTCTCAGATCTGGTTTCTAAGAGTTTTGGGG; GGCGGGGCTGTCACCACGTGCAGTATCTCAAGATATTCAGGTGGCCAGAAGAGCTTGTCA; GCAAGAGGAGGACAGAATTCTCCCAGCGTTAACACAAAATCCATGGGCAGCATGATGGCA; GGTCCTCTGTTGCAAACTCAGTTCCAAAGTCACAGGAAGAAAGCAGAAAGTTCAACTTCC; AAAGGGTTAGGACTCTCCACTCAATGTCTTAGGTCAGGAGTTGTGTCTAGGCTGGAAGAG; CCAAAGAATATTCCATTTTCCTTTCCTTGTGGTTGAAAACCACAGTCAGTGGAGAGATGT; TTGGAAACCACAGTCAGTGGAGCCTGGGTGGTACCCAGGCTTTAGCATTATTGGATGTCA; ATAGCATTGTTTTTGTCATGTAGCTGTTTTAAGAAATCTGGCCCAGGGTGTTTGCAGCTG; TGAGAAGTCACTCACACTGGCCACAAGGACGCTGGCTACTGTCTATTAAAATTCTGATGT; TTCTGTGAAATTCTCAGAGTGTTTAATTGTACTCAATGGTATCATTACAATTTTCTGTAA; GAGAAAATATTACTTATTTATCCTAGTATTCCTAACCTGTCAGAATAATAAATATTGGAA; CCAAGACATGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA; AAAAAAAAAAACTCGAC;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001674 unedited; NNGGAACCGTTCAAAATTTGTAAACGACTCACTATAGGGCGGCCGCGAATCGGCACGAGG; GCGCACTGCACAGCTCTCTTCTCTCGCCGCCGCCCGAGCGCACCCTTCAGCCCGCGCGCC; GGCCGTGAGTCCTCGGTGCTCGCCCGCCGGCCAGACAAACAGCCCGCCCGACCCCGTCCC; GACCCTGGCCGCCCCGAGCGGAGCCTGGAGCAAAATGATGCTTCAACACCCAGGCCAGGT; CTCTGCCTCGGAAGTGAGTGCTTCTGCCATCGTCCCCTGCCTGTCCCCTCCTGGGTCACT; GGTGTTTGAGGATTTTGCTAACCTGACGCCCTTTGTCAAGGAAGAGCTGAGGTTTGCCAT; CCAGAACAAGCACCTCTGCCACCGGATGTCCTCTGCGCTGGAATCAGTCACTGTCAGCGA; CAGACCCCTCGGGGTGTCCATCACAAAAGCCGAGGTAGCCCCTGAAGAAGATGAAAGGAA; AAAGAGGCGACGAGAAAGAAATAAGATTGCAGCTGCAAAGTGCCGAAACAAGAAGAAGGA; GAAGACGGAGTGCCTGCAGAAAGAGTCGGAGAAGCTGGAAAGTGTGAATGCTGAACTGAA; GGCTCAGATTGAGGAGCTCAAGAACGAGAAGCAGCATTTGATATACATGCTCAACCTTCA; TCGGCCCACGTGTATTGTCCGGGCTCAGAATGGGAGGACTCCAGAAGATGAGAGAAACCT; CTTTATCCAACAGATAAAAGAAGGAACATTGCAGAGCTAAGCAGTCGTGGTATGGGGGCG; ACTGGGGAGTCCTCATTGAATCCTCATTTTATACCCAAAACCCTGAAGCCATTGGAGAGC; TGTCTTCCTGTGTACCTCTAGAATCCCGCAGCAGAGACCTAG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001674 unedited; TGTATAAAAAAACCGTTTTTTAGAGAAGAGAAATAAAATATTTTTTTTTTTTTTTTTTTT; TTTTTTTTTTTTTTTTTTTTTTTTTTTTAACAATGACTGGGCTCCAATATTTATTATTCG; GACGGGTTAGGAATACTAGGATAAATAAGTAATTTTTTCTTTTACAAAAAATGGCAAGGA; TACCATTGAGCACAATTAAACCCTCTGAGAATTCCCCAGAAACATCCAATTTTTAATAAA; CAGTATCCAGCGCCCTTGGGGCCAGGGGGAGGGACTTACAAAAGGTGAAAACACCCTGGG; CCAGATTTCTCAAAACAGCTCCCCGACTACCCCCCGGCTTTGGCAACCCCAATAATGCTA; AAACTGGAGACCCACCTCGCCACCTCTGACTGGGACACACCCCAAAACACCACAAACGCC; TGAGACCACCCCCTTCACGACAAGGACCTCGTCCCCCCCCCTACCCATACCCACCCCCCC; ATCTCTCCCTTCCTTATCTCACCCCTCCCCCCCTCCCCCCCTCCCTCCCTACACCTTCCC; CACCCCACTCTGCCCCACCCCCCTTTGTCTGCTCCACCGCCATCCCTTTTACACATCCCC; CCCCACTTTCACTCCCACCCGCACTCTTCCTCTCCAATCACCTCTCTCACCGCCTCTCTT; CTCCCCCCCCCCCCCCCCCCCCGCCACCTCCCCCTCCTCCCCCTTTCTCTCTCCTTCCTC; CCCCCCTCCCTCTTCTTACCCCCTTCCATGTCTCCCCCCACCACCCTCCACTTCCTCACC; CGCCCCCTTTTTTCCCCCGCTCTCTGTATCCCCTGATAATCTCCCATTTTTTCTATTCAC; CCTCACTATCCACCCATTCCACCCCACGCCCTCTTATTATATCTACTCCCTCATCTCTCC; ACTCCTCTCCTCCCTCCCTCTACGTCCGCACGTACTCACCACACTCTACACTGTTCTCAC; ATACTACT;
• Restriction Sites: NotI-NotI
• ACCN: NM_001674
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_001674.2.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001674.2, NP_001665.1
• RefSeq Size: 2049 bp
• RefSeq ORF: 546 bp
• Locus ID: 467
• UniProt ID: P18847
• Cytogenetics: 1q32.3
• Domains: BRLZ
• Protein Families: Transcription Factors
• Summary: This gene encodes a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. This gene is induced by a variety of signals, including many of those encountered by cancer cells, and is involved in the complex process of cellular stress response. Multiple transcript variants encoding different isoforms have been found for this gene. It is possible that alternative splicing of this gene may be physiologically important in the regulation of target genes. [provided by RefSeq, Apr 2011]; Transcript Variant: This variant (1) encodes the longest isoform (1), which represses rather than activates transcription from promoters with ATF-binding elements. Both variants 1 and 3 encode isoform 1.