ARIH2 (NM_006321) Human Untagged Clone

SKU SC108565

ARIH2 (untagged)-Human ariadne homolog 2 (Drosophila) (ARIH2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: ARIH2
• Synonyms: ARI2; TRIAD1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_006321 edited; GAATTCGGCACGAGGCACTTCCGGAGCTGTGGGGACGACTCTTCTGGAGGAAGCAGCGCG; GGCTTGACCGGCGTCGGCCCGCCGCCTCCGCTGCCGCTTCGCCCCAATCCGGTCCCTCTG; GCCCGGCCTGACCCGGTCTGGCTTGTTCGGGCTCAGCGGCCGCGAGGCCGCAGCTCCCGA; TGGAAATCATATTATGTAGAATACTTGGGTGACATCTGCCTGAGAGATCTCCAAGAATTA; CAGATTGAGTCTCGCTCTGTTGCCCAGGCTGGAGTGCAGTGGTGCAATCTCTGCTCACTG; CAACCTCCGTCTCCCGAGTTCAAGCAGTTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGAT; TACAGGTAAGACAAAAATACTAATGCATTTGAGAAAGCGGTAGTTTTGGGGGGAGGGGGA; AAAAGCAACTGCTTTCCTGATCTGCAACTTGGCTGGATGCTAAGATGTCAGTGGACATGA; ATAGCCAGGGGTCTGACAGCAATGAAGAGGACTATGACCCAAATTGTGAGGAAGAGGAAG; AAGAAGAAGAAGACGACCCTGGGGACATAGAGGACTATTACGTGGGAGTAGCCAGCGATG; TGGAGCAGCAGGGGGCTGATGCCTTTGATCCCGAGGAGTACCAGTTCACTTGCTTGACCT; ACAAGGAATCTGAGGGTGCCCTCAATGAGCACATGACCAGCTTAGCTTCTGTCCTAAAGG; TATCTCATTCAGTTGCTAAACTTATATTAGTTAATTTCCACTGGCAAGTTTCAGAGATAT; TGGACAGATACAAGTCCAATTCTGCTCAACTGCTTGTTGAGGCTCGAGTTCAGCCTAATC; CATCAAAACATGTTCCCACATCCCATCCCCCTCACCACTGTGCAGTGTGTATGCAGTTTG; TGCGAAAGGAAAACCTACTCTCTCTGGCCTGTCAGCACCAGTTTTGCCGCAGCTGCTGGG; AGCAGCACTGCTCAGTTCTCGTCAAGGACGGCGTGGGCGTGGGAGTCTCTTGCATGGCTC; AGGACTGTCCACTCCGTACACCAGAGGACTTTGTGTTTCCATTGCTTCCCAATGAAGAAT; TGAGAGAGAAATACAGGCGCTACCTCTTCAGGGACTATGTGGAGAGTCATTACCAGCTCC; AGCTGTGCCCTGGTGCAGACTGCCCCATGGTTATTCGGGTACAGGAGCCTAGAGCTCGCC; GAGTACAGTGCAATCGGTGCAACGAGGTCTTCTGTTTCAAGTGTCGTCAGATGTATCACG; CACCCACAGACTGTGCCACAATCCGGAAATGGCTCACGAAGTGTGCAGACGACTCTGAAA; CAGCCAACTACATTAGTGCTCACACTAAAGACTGTCCCAAGTGCAACATCTGCATTGAGA; AGAATGGAGGCTGCAATCACATGCAATGCTCCAAATGTAAACACGACTTCTGCTGGATGT; GTCTAGGAGATTGGAAGACTCATGGCAGTGAATACTATGAGTGCAGTCGTTACAAGGAGA; ATCCTGACATCGTGAACCAGAGCCAACAAGCCCAGGCGAGGGAAGCCCTCAAGAAGTACT; TATTCTACTTTGAGAGGTGGGAAAACCACAATAAAAGCTTGCAGCTAGAGGCACAGACAT; ACCAGCGGATTCACGAGAAGATTCAGGAGAGGGTCATGAACAATCTGGGGACATGGATCG; ACTGGCAGTACCTACAGAATGCTGCCAAGCTCTTGGCCAAGTGTCGATACACCCTGCAAT; ACACCTACCCATATGCATATTACATGGAGTCCGGACCCAGGAAGAAGCTGTTTGAATACC; AGCAGGCTCAGCTGGAGGCTGAGATCGAAAACCTCTCATGGAAAGTGGAGCGTGCAGACA; GCTATGACAGAGGGGACTTGGAGAACCAGATGCATATAGCGGAGCAGCGGAGGAGAACCC; TGCTGAAAGATTTCCATGACACCTAAGTTGGGATGTGGATGTGCCGGGGTGAGGAAGATG; TGGCTGCAAGGTCTCCCGGCTGCCATACTGCATGCTGCAGGCTCTGCCTTTCATGACCCC; AGGCAACAGCCAGGGCCCCACTCCTGAGAGACACTGGCAACACCTCTTAGTTGATTTCTG; TTTTCTTCTCTTTTCACTTTTTGTTTCTACCAGGGTAGAGGCCATGTTGAACTGGCCTCT; TTTCAGGACTTTTATTTCCCCCTGGATGGTTGTTGGGAGGGAGGGAAAGTGTTTTCTGAA; TGGCTATTAATAGTATTAGATCATTACAACTTATGTAACTTTCAAAGGTTGTACAATTAT; ACAAAAAAAAAAAAAAAAAACTCGAC;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006321 unedited; TTTTGTAATACGACTTACTATAGGGCGGCCGCGAATTCGGCACGAGGCACTTCCGGAGCT; GTGGGGACGACTCTTCTGGAGGAAGCAGCGCGGGCTTGACCGGCGTCGGCCCGCCGCCTC; CGCTGCCGCTTCGCCCCAATCCGGTCCCTCTGGCCCGGCCTGACCCGGTCTGGCTTGTTC; GGGCTCAGCGGCCGCGAGGCCGCAGCTCCCGATGGAAATCATATTATGTAGAATACTTGG; GTGACATCTGCCTGAGAGATCTCCAAGAATTACAGATTGAGTCTCGCTCTGTTGCCCAGG; CTGGAGTGCAGTGGTGCAATCTCTGCTCACTGCAACCTCCGTCTCCCGAGTTCAAGCAGT; TCTCCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTAAGACAAAAATACTAATGCAT; TTGAGAAAGCGGTAGTTTTGGGGGGAGGGGGAAAAAGCAACTGCTTTCCTGATCTGCAAC; TTGGCTGGATGCTAAGATGTCAGTGGACATGAATAGCCAGGGGTCTGACAGCAATGAAGA; GGACTATGACCCAAATTGTGAGGAAGAGGAAGAAGAAGAAGAAGACGACCCTGGGGACAT; AGAGGACTATTACGTGGGAGTAGCCAGCGATGTGGAGCAGCAGGGGGCTGATGCCTTTGA; TCCCGAGGAGTACCAGNTCACTTGCTTGACCTACAAGGAATCTGAGGGTGCCCTCAATGA; GCACATGACCAGCTTAGCTTCTGTCCTAAAGGTATCTCATTCAGTTGCTAAACTTATATT; AGTTAATTTCCCACTGGCAGTTTCAGAGATATTGGACAGATACAAGTCCAATTCTGCTCA; ACTGCCTGTTGAGCCTCCAGNTCACCCTAACCATCAAAAATGTTCCCCA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006321 unedited; CAGAGTCGAGTTTTTTTTTTTTTTTTTTGTATAATTGTACAACCTTTTGAAAGTTACATA; AGTTGTAATGATCTAATACTATTAATAGCCATTCAGAAAACACTTTCCCTCCCTCCCAAC; AACCATCCAGGGGGAAATAAAAGTCCTGAAAAGAGGCCAGTTCAACATGGCCTCTACCCT; GGTAGAAACAAAAAGTGAAAATAGAAGAAAACAGAAATCAACTAAGAGGTGTTGCCAGTG; TCTCTCAGGAGTGGGGCCCTGGCTGTTGCCTGGGGTCATGAAAGGCAGAGCCTGCAGCAT; GCAGTATGGCAGCCGGGAGACCTTGCAGCCACATCTTCCTCACCCCGGCACATCCACATC; CCAACTTAGGTGTCATGGAAATCTTTCAGCAGGGTTCTCCTCCGCTGCTCCGCTATATGC; ATCTGGTTCTCCAAGTCCCCTCTGTCATAGCTGTCTGCACCCTCCACTTTCCATGACAGG; TTTTCGATTCTCAGCTCCACCTGAGCCTGCTGGTATTCAAACAGCTTCTTCCTGGGTCCC; GACTCCTTGTAATATGCATATGGGTACGGTGTCTTGCAGGTTGTTTCGACACTTGGCCCA; GAACTTTGGACCATTTCTGTAGGCACTGCCAGTTGTATCTTGTCCCCAAGATGTCCATGA; CCCTCCTCTGAATCTTCCTCTGAACCCGGTGGATTTTCTGGCCTTCACCTCTAACCTTTA; TTGGGTTTTCTCCCACTCCCCCCCTAAAACAATCACTCTTGTATGGCTCTCCTTTCCCGG; TTCTGTCGGTTTGGTCCACCATTAACGACTTCCCCTTGTACACCCGCCCATATTGTACCC; ATGCCTGCTCCTCCCCCCTCTTTTTACCCTCCCCCTAACCGCCGTCTCCTCTCGCCTCCT; CTTGTCCCGTCCGTTCTTCTTCTCATGTCCATTTTCTCCCGTATCTTTTCTCCCGTTTAC; CTTGTTTCCGCCTCTCCATATATCTTTCCCTTCCCGACCTATTTTCTCCTCGCCCCTCTT; GTTTGTTTCACCTTACACTTCGAACACCAAATTTCTTCN;
• Restriction Sites: NotI-NotI
• ACCN: NM_006321
• Insert Size: 2490 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006321.1, NP_006312.1
• RefSeq Size: 3892 bp
• RefSeq ORF: 1482 bp
• Locus ID: 10425
• UniProt ID: O95376
• Cytogenetics: 3p21.31
• Domains: IBR
• Protein Families: Druggable Genome
• Summary: The protein encoded by this gene is an E3 ubiquitin-protein ligase that polyubiquitinates some proteins, tagging them for degradation. The encoded protein upregulates p53 in some cancer cells and may inhibit myelopoiesis. Several transcript variants encoding different isoforms have been found for this gene, although the full-length nature of some of them have not been determined yet. [provided by RefSeq, Nov 2015]; Transcript Variant: This variant (2), as well as variants 1, 4, 5, and 6, encodes isoform a.