RBM5 (NM_005778) Human Untagged Clone

SKU SC108553

RBM5 (untagged)-Human RNA binding motif protein 5 (RBM5), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: RBM5
• Synonyms: G15; H37; LUCA-15; LUCA15; RMB5
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108553 sequence for NM_005778 edited (data generated by NextGen Sequencing); ATGGGTTCAGACAAAAGAGTGAGTAGAACAGAGCGTAGTGGAAGATACGGTTCCATCATA; GACAGGGATGACCGTGATGAGCGTGAATCCCGAAGCAGGCGGAGGGACTCAGATTACAAA; AGATCTAGTGATGATCGGAGGGGTGATAGATATGATGACTACCGAGACTATGACAGTCCA; GAGAGAGAGCGTGAAAGAAGGAACAGTGACCGATCCGAAGATGGCTACCATTCAGATGGT; GACTATGGTGAGCACGACTATAGGCATGACATCAGTGACGAGAGGGAGAGCAAGACCATC; ATGCTGCGCGGCCTTCCCATCACCATCACAGAGAGCGATATTCGAGAAATGATGGAGTCC; TTCGAAGGCCCTCAGCCTGCGGATGTGAGGCTGATGAAGAGGAAAACAGGTGTAAGCCGT; GGTTTCGCCTTCGTGGAGTTTTATCACTTGCAAGATGCTACCAGCTGGATGGAAGCCAAT; CAGAAAAAGTTGGTGATTCAAGGAAAGCACATTGCAATGCATTATAGCAATCCCAGACCT; AAGTTTGAAGATTGGCTTTGTAACAAGTGCTGCCTTAACAATTTCAGGAAAAGACTAAAA; TGCTTCCGATGTGGAGCAGACAAGTTTGACTCTGAACAGGAAGTGCCTCCTGGAACCACA; GAGTCGGTTCAGTCTGTGGATTACTACTGTGATACGATCATTCTTCGGAACATAGCTCCG; CACACTGTGGTGGATTCCATCATGACAGCACTGTCTCCTTACGCGTCTTTAGCTGTCAAT; AACATCCGCCTCATAAAAGACAAACAGACCCAGCAGAACAGAGGCTTCGCATTTGTGCAG; CTGTCCTCTGCAATGGATGCTTCTCAGCTGCTTCAGATATTACAGAGTCTCCATCCTCCT; TTGAAAATTGATGGCAAAACTATTGGGGTTGATTTTGCAAAAAGTGCCAGAAAAGACTTG; GTCCTCTCAGATGGTAACCGCGTCAGCGCCTTCTCTGTAGCTAGTACGGCTATTGCTGCT; GCTCAGTGGTCATCCACCCAGTCTCAAAGTGGTGAAGGAGGCAGTGTTGACTACAGTTAT; CTGCAACCAGGTCAAGATGGCTATGCCCAATATGCTCAGTATTCACAGGATTATCAGCAG; TTTTATCAACAACAAGCTGGAGGATTGGAATCTGATGCATCATCTGCATCAGGCACAGCA; GTGACCACCACCTCAGCGGCTGTAGTGTCCCAGAGTCCTCAGCTGTATAATCAAACCTCC; AATCCACCTGGCTCTCCGACTGAGGAAGCACAGCCTAGCACTAGCACAAGTACACAGGCC; CCAGCCGCTTCCCCTACTGGTGTAGTTCCTGGTACCAAATATGCAGTACCTGACACGTCC; ACTTACCAGTATGATGAATCTTCAGGATATTACTATGATCCGACAACAGGGCTCTATTAT; GACCCCAACTCGCAATACTACTATAATTCCTTGACCCAGCAGTACCTTTACTGGGATGGG; GAAAAAGAGACCTACGTGCCAGCTGCAGAGTCTAGCTCCCACCAGCAGTCGGGCCTGCCT; CCTGCAAAAGAGGGGAAAGAGAAGAAGGAGAAACCCAAGAGCAAAACAGCCCAGCAGATT; GCCAAAGACATGGAACGCTGGGCTAAGAGTTTGAATAAGCAGAAAGAAAACTTTAAAAAT; AGCTTTCAGCCTGTCAATTCCTTGAGGGAAGAAGAAAGGAGAGAATCTGCTGCAGCAGAC; GCTGGCTTTGCTCTCTTTGAGAAGAAGGGAGCCTTAGCTGAAAGGCAGCAGCTCATCCCA; GAATTGGTGCGAAATGGAGATGAGGAGAATCCCCTCAAAAGGGGTCTGGTTGCTGCTTAC; AGTGGTGACAGTGACAATGAGGAGGAGCTGGTGGAGAGACTTGAGAGTGAGGAAGAGAAG; CTAGCTGACTGGAAGAAGATGGCCTGTCTGCTCTGCCGGCGCCAGTTCCCGAACAAAGAT; GCCCTAGTCAGGCACCAGCAACTCTCAGACCTTCACAAGCAAAACATGGACATCTACCGA; CGATCCAGGCTGAGCGAGCAGGAGCTGGAAGCCTTGGAGCTAAGGGAGAGAGAGATGAAA; TACCGAGACCGAGCTGCAGAAAGACGGGAGAAGTACGGCATTCCAGAACCTCCAGAGCCC; AAGCGCAAGAAGCAGTTTGATGCCGGCACTGTGAATTACGAGCAACCCACCAAAGATGGC; ATTGACCACAGTAACATTGGCAACAAGATGCTGCAGGCCATGGGCTGGCGGGAAGGCTCT; GGCTTGGGACGAAAGTGTCAAGGCATTACGGCTCCCATTGAGGCTCAAGTTCGGCTAAAG; GGAGCTGGCCTAGGAGCCAAAGGCAGCGCATATGGTTTGTCGGGCGCCGATTCCTACAAA; GATGCTGTCCGGAAAGCCATGTTTGCCCGGTTCACTGAGATGGAGTGA; ; Clone variation with respect to NM_005778.2; 990 t=>c;2037 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005778 unedited; GGTCAAATTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGACGAGGCCTTGTTGGAG; GTTCTGGGGCGCAGAACCGCTACTGCTGCTTCGGTCTCTCCTTGGNGAAAAAATAAAATT; TGAACCTTTTGGAGCTGTGTGCTAAATCTTCAGTGGGACAATGGGTTCAGACAAAAGAGT; GAGTAGAACAGAGCGTAGTGGAAGATACGGTTCCATCATAGACAGGGATGACCGTGATGA; GCGTGAATCCCGAAGCAGGCGGAGGGACTCAGATTACAAAAGATCTAGTGATGATCGGAG; GGGTGATAGATATGATGACTACCGAGACTATGACAGTCCAGAGAGAGAGCGTGAAAGAAG; GAACAGTGACCGATCCGAAGATGGCTACCATTCAGATGGTGACTATGGTGAGCACGACTA; TAGGCATGACATCAGTGACGAGAGGGAGAGCAAGACCATCATGCTGCGCGGCCTTCCCAT; CACCATCACAGAGAGCGATATTCGAGAAATGATGGAGTCCTTCGAAGGCCCTCAGCCTGC; GGATGTGAGGCTGATGAAGAGGAAAACAGGTGTAAGCCGTGGTTTCGCCTTCGTGGAGTT; TTATCACTTGCAAGATGCTACCAGCTGGATGGAAGCCAATCAGAAAAAGTTGGTGATTCA; AGGAAAGCACATTGCAATGCATTATAGCAATCCCAGACCTAAGTTTGAAGATTGGCTTTG; TAACAAGTGCTGCCTTAACAATTTCAGGAAAGACTAAAATGCTTCCGATGTGGAGCAGAC; CAGTTTGACTCTGAACAGAAAGTGCCTCCTGGGACCACGAGTCGGTCAGTCTGTGGATAC; TACTGTGATCGAACTTTCTTCGAACTAACTCCGCCACTGTGGGGAATCCATCATGACAGA; CTGTCTCTTACCCC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005778 unedited; CTACAGGCTCATTTTNCTTCTCTGTTTAAGGGAGNCCAGNCATTTGGCAACTAGNACTGN; AAAATCTTCCAAAATTCTGTACAAATGTGTAATATACAAATATACACAAGGCTTTTGCCA; AGAAAAGACATCACAACAAATGACAGAGGTGGCTTTGGTGTCACATACCACCTACAGCAT; CTCAAACCCCTAGGAATGTGAGGGGCTGATGGACCACAACATAGGCTACACATGTAAACA; CGCTACATTACACTATGTACACTGGAGTATAAACAATCCACTGTCCTCTCCGGTCTGTTC; ACAAGCCCTCTGGCGGGAAGGAAGGCCATTCACCAGGCAACCCACATAAGGGGAACTTCT; TTAAGGTGGGAGGGAGAACCCTGCAGAATGAAGTGGTTCACCCTAAGATCTATTAACAGC; ACAAGGCATGCCCTTAAAGAGACAGGCGGTAACAGCGAATTCGGGAGATGGACCACTTCT; TGTGCTCCTTGTCATCTCTCTCTCTCTCTCTCTCTCACTCCATCTCAGTGAACCGGGCAA; ACATGGCTTTCCGGACAGCATCTTTGTAGGAATCGGCGCCCGACAAACCATATGCGCTGC; CTTTGGCTCCTAGGCCAGCTCCCTTTAGCCGAACTTGAGCCTCAATGGGAGCCGTAATGC; CTTGACACTTTCGTCCCAAGCCAGAGCCTTTCCGCCAGCCCATGGCCTGCAGCATCTTGT; TGCCAATGTTACTGTGGTCAATGCCATCTTTGGTGGCTGGCTCCGTAATTACAGTGCCCG; CATAAACTGCTTCTTGCCCCTGGGCTCTGGAGGTTCTGGAATGCCGTACCTTTTCCCCCT; TTCTTGCAGCTTGGGCCTNGGGATTTAATCTCCTTCTCCCTTACCTCCCAGGTTTCCATT; CCTTGCTTGTCTAACCTGGATACTCCGCCAAAGTCCCTTGTTTGCTTTTGAAGGTCTAAA; AATTCTTGCGCCCGACTAGGGCATTTTTTCTTGGAACTGCGCCCGCCTACACN;
• Restriction Sites: NotI-NotI
• ACCN: NM_005778
• Insert Size: 3100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005778.1, NP_005769.1
• RefSeq Size: 3135 bp
• RefSeq ORF: 2448 bp
• Locus ID: 10181
• UniProt ID: P52756
• Cytogenetics: 3p21.31
• Domains: G-patch, RRM, zf-C2H2, zf-RanBP
• Protein Families: Druggable Genome
• Summary: This gene is a candidate tumor suppressor gene which encodes a nuclear RNA binding protein that is a component of the spliceosome A complex. The encoded protein plays a role in the induction of cell cycle arrest and apoptosis through pre-mRNA splicing of multiple target genes including the tumor suppressor protein p53. This gene is located within the tumor suppressor region 3p21.3, and may play a role in the inhibition of tumor transformation and progression of several malignancies including lung cancer. [provided by RefSeq, Oct 2011]; Transcript Variant: This variant (1) represents the longer transcript and is protein coding.