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EIF3B (NM_003751) Human Untagged Clone

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SKU
SC108421
EIF3B (untagged)-Human eukaryotic translation initiation factor 3, subunit B (EIF3B), transcript variant 1
  $873.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol EIF3B
Synonyms EIF3-ETA; EIF3-P110; EIF3-P116; EIF3S9; PRT1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_003751, the custom clone sequence may differ by one or more nucleotides


ATGCAGGACGCGGAGAACGTGGCGGTGCCCGAGGCGGCCGAGGAGCGCGCCGAGCCCGGCCAGCAGCAGC
CGGCCGCCGAGCCGCCGCCAGCCGAGGGGCTGCTGCGGCCCGCGGGGCCCGGCGCTCCGGAGGCCGCGGG
GACCGAGGCCTCCAGTGAGGAGGTGGGGATCGCGGAGGCCGGGCCGGAGTCCGAGGTGAGGACCGAGCCG
GCGGCCGAGGCAGAGGCGGCCTCCGGCCCGTCCGAGTCGCCCTCGCCGCCGGCCGCCGAGGAGCTGCCCG
GGTCGCATGCTGAGCCCCCTGTCCCGGCACAGGGCGAGGCCCCAGGAGAGCAGGCTCGGGACGAGCGCTC
CGACAGCCGGGCCCAGGCGGTGTCCGAGGACGCGGGAGGAAACGAGGGCAGAGCGGCCGAGGCCGAACCC
CGGGCGCTGGAGAACGGCGACGCGGACGAGCCCTCCTTCAGCGACCCCGAGGACTTCGTGGACGACGTGA
GCGAGGAAGAATTACTGGGAGATGTACTCAAAGATCGGCCCCAGGAAGCAGATGGAATCGATTCGGTGAT
TGTAGTGGACAATGTCCCTCAGGTGGGACCCGACCGACTTGAGAAACTCAAAAATGTCATCCACAAGATC
TTTTCCAAGTTTGGGAAAATCACAAATGATTTTTATCCTGAAGAGGATGGGAAGACAAAAGGGTATATTT
TCCTGGAGTACGCGTCCCCTGCCCACGCTGTGGATGCTGTGAAGAACGCCGACGGCTACAAGCTTGACAA
GCAGCACACATTCCGGGTCAACCTCTTTACGGATTTTGACAAGTATATGACGATCAGTGACGAGTGGGAT
ATTCCAGAGAAACAGCCTTTCAAAGACCTGGGGAACTTACGTTACTGGCTTGAAGAGGCAGAATGCAGAG
ATCAGTACAGTGTGATTTTTGAGAGTGGAGACCGCACTTCCATATTCTGGAATGACGTAAAAGACCCTGT
CTCAATTGAAGAAAGAGCGAGATGGACAGAGACGTATGTGCGTTGGTCTCCTAAGGGCACCTACCTGGCT
ACCTTTCATCAAAGAGGCATTGCTCTATGGGGGGGAGAGAAATTCAAGCAAATTCAGAGATTCAGCCACC
AAGGGGTTCAGCTTATTGACTTCTCACCTTGTGAAAGGTACCTGGTGACCTTTAGCCCCCTGATGGACAC
GCAGGATGACCCTCAGGCCATAATCATCTGGGACATCCTTACGGGGCACAAGAAGAGGGGTTTTCACTGT
GAGAGCTCAGCCCATTGGCCTATTTTTAAGTGGAGCCATGATGGCAAATTCTTTGCCAGAATGACCCTGG
ATACGCTTAGCATCTATGAAACTCCTTCTATGGGTCTTTTGGACAAGAAGAGTTTGAAGATCTCTGGGAT
AAAAGACTTTTCTTGGTCTCCTGGTGGTAACATAATCGCCTTCTGGGTGCCTGAAGACAAAGATATTCCA
GCCAGGGTAACCCTGATGCAGCTCCCTACCAGGCAAGAGATCCGAGTGAGGAACCTGTTCAATGTGGTGG
ACTGCAAGCTCCATTGGCAGAAGAACGGAGACTACTTGTGTGTGAAAGTAGATAGGACTCCGAAAGGCAC
CCAGGGTGTTGTCACAAATTTTGAAATTTTCCGAATGAGGGAGAAACAGGTACCTGTGGATGTGGTCGAG
ATGAAAGAAACCATCATAGCCTTTGCCTGGGAACCAAATGGAAGTAAGTTTGCTGTGCTGCACGGAGAGG
CTCCGCGGATATCTGTGTCTTTCTACCACGTCAAAAACAACGGGAAGATTGAACTCATCAAGATGTTCGA
CAAGCAGCAGGCGAACACCATCTTCTGGAGCCCCCAAGGACAGTTCGTGGTGTTGGCGGGCCTGAGGAGT
ATGAACGGTGCCTTAGCGTTTGTGGACACTTCGGACTGCACGGTCATGAACATCGCAGAGCACTACATGG
CTTCCGACGTCGAATGGGATCCTACTGGGCGCTACGTCGTCACCTCTGTGTCCTGGTGGAGCCATAAGGT
GGACAACGCGTACTGGCTGTGGACTTTCCAGGGACGCCTCCTGCAGAAGAACAACAAGGACCGCTTCTGC
CAGCTGCTGTGGCGGCCCCGGCCTCCCACACTCCTGAGCCAGGAACAGATCAAGCAAATTAAAAAGGATC
TGAAGAAATACTCTAAGATCTTTGAACAGAAGGATCGTTTGAGTCAGTCCAAAGCCTCAAAGGAATTGGT
GGAGAGAAGGCGCACCATGATGGAAGATTTCCGGAAGTACCGGAAAATGGCCCAGGAGCTCTATATGGAG
CAGAAAAACGAGCGCCTGGAGTTGCGAGGAGGGGTGGACACTGACGAGCTGGACAGCAACGTGGACGACT
GGGAAGAGGAGACCATTGAGTTCTTCGTCACTGAAGAAATCATTCCCCTCGGGAATCAGGAGTGA

5' Read Nucleotide Sequence
>OriGene 5' read for NM_003751 unedited
CTCACTATAGGGCGGCCGCGAATTCGGCACGAGGAGCGCGGCGGCCGCGGAGCCCTGCGA
GTAGGCAGCGTTGGGCCCATGCAGGACGCGGAGAACGTGGCGGTGCCCGAGGCGGCCGAG
GAGCGCGCCGAGCCCGGCCAGCAGCAGCCGGCCGCCGAGCCGCCGCCAGCCGAGGGGCTG
CTGCGGCCCGCGGGGCCCGGCGCTCCGGAGGCCGCGGGGACCGAGGCCTCCAGTGAGGAG
GTGGGGATCGCGGAGGCCGGGCCGGAGCCCGAGGTGAGGACCGAGCCGGCGGCCGAGGCA
GAGGCGGTCTCCGGCCCGTCCGAGTCGCCCTCGCCGCCGGCCGCCGAGGAGCTGCCCGGG
TCGCATGCTGAGCCCCCTGTCCCGGCACAGGGCGAGGCCNCAGGAGAGCAGGCTCGGGAC
GAGCGCTCCGACAGCCGGGCCCAGGCGGTGTCCGAGGACGCGGGAGGAAACGAGGGCAGA
GCGGCCGAGGCCGAACCACGGACGCTGGAGAACGGCGACGCNGACGAGCCCTNCTTCATC
GACCCCCGAGACTTCGTGGACGACGTGAGCGAGGAAGGAATACTGGAGATGTACTCAGAG
ATCCGGCCCAGGAATCAGATGGAACTCGATCGGTGATTGCAGTGGACAATGTCCCTCATG
TGGGTACCCGACCGACTCGAGAAACTCAGAAATGTCATCCACATGATCCTTTCCAAGCTT
GCGACAATCACAAATGATTTTTATGCTGAAGAGGATGGGAAGACAAAAGGGTCTATGTTA
CTGGAGTACGCGTCCCCTGCCACCGCTGAGATGCTGTGAATACGCCGACGCNTACAGCCT
GACAGCACACACATTCCGGTCAACCTCTTACGGTTTGACAGTTATGACGATCGTGACAGT
GGGATCTGCAAGGACAGCTTTAAGACTGGGGACTACGTACTGCTTGAGAGCNTAAGCNGA
ATCATCAGGGGATTTTTAAGGGAAACGGCTCCTACTCGG
3' Read Nucleotide Sequence
>OriGene 3' read for NM_003751 unedited
CCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTAATACAAGTTTGCTTTTATTTCCA
TGTTCAACCTGCGGAGAAAACCGGCCACAACCTGCACGGTGGGGGTACAAGGCATGGCGG
CGCCCTCCCGGTGGGTCTCCGGGACACGTCCAGCACTGCCCGCAATGGGGATGCAAGTCC
TTCAACGGAAATGCTGCTCGGCTGTGTACAGGCACCGTGGAAACATCACTGGGCGCAGGA
GAAGGTGCCGCCAGTGGCGTCGCTAAGTCTTCGGAGCCAACGCCGGCGGTTCCAACAGAA
AACTCCAAACAAGAAAGAAGTGGAAGCGGGTGCCTTAAATCCCCCACTGCAGACACCAGG
GGCTGAAAGGCACAAAAATGTGTCGCAATGGCAGAATCCAGGCGCAGTCACAGTCCAGAG
CCAGAGAGCACAGGGAGGAGGCGGGAGTCACGCGGCTTCCTGCAGGACGGCCTCGGCTCC
ACAGCACACACGGCACAGTGCTCCAGGTCACTCCTGATTCCCGAGGGGAATGATTTCTTC
AGTGACGAAGAACTCAATGGTCTCCTCTTCCCAGTCGTCCACGTTGCTGTCCAGCTCGTC
AGTGTCCACCCCTCCTCGCAACTCCAGGCGCTCGTTGTTCTGCTCCATATAGAGCTCCTG
GGCCTTTTTCCGGTACTTCCGGAAATCTTCCATCATGGGGCGCCTTCTCTCCACCATTCC
CTTGGAGGCTTTGGACTGACTCAAACGATCCTTCTGTTCAAAGATCTAGAGTATTCTTTC
AGAACCTTTTCAATATGCTCGAACCGGTCCCTGGCTCAAGAGTGTGGGAAGCCGGGGCCC
CCCCACCATCTGGCATAACCGTCCCTGATGTTCTTTTGCAGAAGGGTCCTGAAAGGTCCA
AACATCACCGTGGCCACTCATGCTTCCCAAAACAAAGGGACACTTACCCCAATAGAACCC
TCCACCCGGAACCTTTTGCCTCGCAGGTATACCC
Restriction Sites NotI-NotI |
ACCN NM_003751
Insert Size 3000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003751.2, NP_003742.2
RefSeq Size 3009 bp
RefSeq ORF 3009 bp
Locus ID 8662
UniProt ID P55884
Cytogenetics 7p22.3
Domains RRM
Summary RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:9388245, PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:9388245, PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) differs in the 3' UTR compared to variant 2. Variants 1, 2 and 3 encode the same isoform (1).
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Documents
Resources
"NM_003751" in other vectors (4)
SKU Description Size Price
RC211093 EIF3B (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 3, subunit B (EIF3B), transcript variant 1 10 ug
$745.00
RC211093L3 Lenti ORF clone of Human eukaryotic translation initiation factor 3, subunit B (EIF3B), transcript variant 1, Myc-DDK-tagged 10 ug
$1,045.00
RC211093L4 Lenti ORF clone of Human eukaryotic translation initiation factor 3, subunit B (EIF3B), transcript variant 1, mGFP tagged 10 ug
$1,045.00
RG211093 EIF3B (tGFP-tagged) - Human eukaryotic translation initiation factor 3, subunit B (EIF3B), transcript variant 1 10 ug
$945.00

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