EIF2B4 (NM_015636) Human Untagged Clone

SKU SC108336

EIF2B4 (untagged)-Human eukaryotic translation initiation factor 2B, subunit 4 delta, 67kDa (EIF2B4), transcript variant 3

Product Data

• Type: Human Untagged Clone
• Target Symbol: EIF2B4
• Synonyms: EIF-2B; EIF2B; EIF2Bdelta
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_015636, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGCTGCTGTGGCCGTGGCTGTTCGCGAGGACTCGGGATCCGGGATGAAGGCGGAGCTTCCCCCTGGGC; CTGGGGCAGTGGGGAGGGAAATGACCAAAGAAGAAAAGCTGCAGCTTCGGAAGGAAAAGAAACAGCAGAA; GAAGAAACGGAAGGAAGAAAAGGGGGCAGAACCAGAGACTGGCTCTGCTGTATCTGCAGCCCAATGTCAA; GGCCCAACCAGAGAACTGCCAGAATCGGGCATTCAGTTGGGCACTCCTCGGGAGAAAGTTCCAGCTGGTC; GGAGTAAGGCCGAACTTCGGGCTGAGCGTCGAGCCAAGCAGGAGGCCGAGCGGGCCCTGAAACAGGCAAG; AAAAGGGGAACAAGGAGGACCACCTCCTAAGGCCAGCCCCAGCACAGCTGGAGAAACCCCCTCAGGAGTG; AAGCGTCTCCCTGAGTACCCTCAGGTTGATGACCTACTTCTGAGAAGGCTTGTTAAAAAACCAGAGCGTC; AACAGGTTCCTACACGAAAGGATTATGGATCCAAAGTCAGTCTCTTCTCTCACCTACCCCAGTACAGCAG; ACAAAACTCTCTGACCCAGTTTATGAGCATCCCATCCTCTGTGATCCACCCAGCCATGGTGCGACTCGGC; CTGCAGTACTCCCAGGGCCTGGTCAGTGGCTCCAATGCCCGGTGTATTGCCCTGCTTCGTGCCTTGCAGC; AGGTGATTCAGGATTACACAACACCGCCTAATGAAGAACTCTCCAGGGATCTAGTGAATAAACTAAAACC; CTACATGAGCTTCCTGACTCAGTGCCGTCCCCTGTCAGCGAGCATGCACAACGCCATCAAGTTCCTTAAC; AAGGAAATCACCAGTGTGGGCAGTTCCAAGCGGGAAGAGGAGGCCAAGTCAGAACTTCGAGCAGCCATTG; ATCGGTATGTGCAAGAGAAGATTGTGCTAGCAGCTCAGGCAATTTCACGCTTTGCTTACCAGAAGATCAG; TAATGGAGATGTGATCCTGGTATATGGATGCTCATCTCTGGTATCACGAATTCTTCAGGAGGCTTGGACA; GAGGGCCGGCGGTTTCGGGTGGTAGTGGTGGACAGCCGGCCATGGCTGGAAGGAAGGCACACACTACGTT; CTCTAGTCCATGCTGGTGTCCCAGCCTCCTACCTGCTGATTCCTGCAGCCTCCTATGTGCTCCCAGAGGT; TTCCAAGGTGCTATTGGGAGCTCATGCACTCTTGGCCAACGGGTCTGTGATGTCACGGGTAGGGACAGCA; CAGTTAGCCCTGGTGGCTCGAGCCCATAATGTACCAGTGCTGGTTTGCTGTGAAACATACAAGTTCTGTG; AGCGTGTGCAGACTGATGCCTTTGTCTCTAATGAGCTAGATGACCCTGATGATCTGCAATGTAAGCGGGG; AGAACATGTTGCGCTGGCTAACTGGCAGAACCACGCATCCCTACGGTTGTTGAATCTAGTCTATGATGTG; ACTCCCCCAGAGCTTGTGGATCTGGTGATCACGGAGCTGGGGATGATCCCTTGCAGTTCTGTACCTGTTG; TTCTACGAGTCAAGAGCAGTGACCAGTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015636 unedited; ACGACTCACTATAGGGCGGCCGCGAATTCGGCACGGGGCGGCTCAGCCTCCACCCGGTGG; CGCTGTGGACCCTGCAGCCGCGGAGCGAGGCAGCGGGGCGGCTCCGTGACCACGGCGGGC; GGAGCCTAGGACTGAGGGCGATGGCTGCTGTGGCCGTGGCTGTTCGCGAGGACTCGGGAT; CCGGGATGAAGGCGGAGCTTCCCCCTGGGCCTGGGGCAGTGGGGAGGGAAATGACCAAAG; AAGAAAAGCTGCAGCTTCGGAAGGAAAAGAAACAGCAGAAGAAGAAACGGAAGGAAGAAA; AGGGGGCAGAACCAGAGACTGGCTCTGCTGTATCTGCAGCCCAATGTCAAGGCCCAACCA; GAGAACTGCCAGAATCGGGCATTCAGTTGGGCACTCCTCGGGAGAAAGTTCCAGCTGGTC; GGAGTAAGGCCGAACTTCGGGCTGAGCGTCGAGCCAAGCAGGAGGCCGAGCGGGCCCTGA; AACAGGCAAGAAAAGGGGAACAAGGAGGACCACCTCCTAAGGCCAGCCCCAGCACAGCTG; GAGAAACCCCCTCAGGAGTGAAGCGTCTCCCTGAGTACCCTCAGGTTGATGACCTACTTC; TGAGAAGGCTTGTTAAAAAACCAGAGCGTCAACAGGTAGGAAGTGGGTTTGGTGCCTGGG; CTAGAAGAGCCTANGGAAATGGACTGGANGGAGGAAAGGTGCNTGGTGCANAGTGAGAAG; GATAGGTGCTTGGGTCANAGTAGTCTTACTGGTCACTTCCTTTCAGCNTTATCCCATCCT; TTNTAAACTCTNGGCCATNGNTCCTGTNCCCTTGNNTTGTCAGGNTCCTACACGAAGGAT; TATGGATCCAAGTCAGTCTCTCTCTCACTACCCNAGTCAGCAGACAAACTCTCTGACCAG; TNATGAGGTAGATCCTATGAANTGNCATACTTTGAGTCTGAGAGTTTANTAGTCGTATGG; AAGTCTATATCTGAATGACTTG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_015636 unedited; ACCTCAGAGNACCAGGCCGCTTTCTAAGATCGGTTTTTTTTTTTTTTTTTTGCTGAGGGT; AGGGAGTATGGCATTTATTAACCCTGTGTTTCCCCCGTCACTGGTCACTGCTCTTGACTC; GTAGAACAACAGGTACAGAACTGCAAGGGATCATCCCCAGCTCCGTGATCACCAGATCCA; CAAGCTCTGGGGGAGTCACATCATAGACTAGATTCAACAACCGTAGGGATGCGTGGTTCT; GCCAGTTAGCCAGCGCAACATGTTCTCCCCGCTTACATTGCAGATCATCAGGGTCATCTA; GCTCATTAGAGACAAAGGCATCAGTCTGCACACGCTCACAGAACTTGTATGTTTCACAGC; AAACCAGCACTGGTACATTATGGGCTCGAGCCACCAGGGCTAACTGTGCTGTCCCTACCC; GTGACATCACAGACCCGTTGGCCAAGAGTGCATGAGCTCCCAATAGCACCTTGGAAACCT; CTGGGAGCACATAGGAGGCTGCAGGAATCAGCAGGTAGGAGGCTGGGACACCAGCATGGA; CTAGAGAACGTAGTGTGTGCCTTCCTTCCAGCCATGGCCGGCTGTCCACCACTACCACCC; GAAACCGCCGGCCCTCTGTCCAAGCCCCCTGAAGAATTCGTGATACCAGAGATGAGCATC; CATATACCAGGATCACATCTCCATTACTGATCTTCTGGTAAGCAAAGCGTGAAATTGCCT; GAGCTGCTAGCACAATCTTTCTCTTGCCCATACCGATCAATGGCTGCTCGAAGTTCTGAC; CTGGCCTAAATGGAGTAAAATCCCTAACCGAACAAGGAAATGGACACCTTTATACCCTCT; GATGATCATGGCCAGGGGCCTGAATTTGCCGTATGATGACAAGCCCATAGAAGGCTCANA; GAAGGCCACAGTCATAATGGCCAACCATTCCTTTACCCCTGCATAGGGCAACAAAC;
• Restriction Sites: NotI-NotI
• ACCN: NM_015636
• Insert Size: 2320 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_015636.2, NP_056451.2
• RefSeq Size: 1643 bp
• RefSeq ORF: 1569 bp
• Locus ID: 8890
• UniProt ID: Q9UI10
• Cytogenetics: 2p23.3
• Domains: IF-2B
• Summary: Eukaryotic initiation factor 2B (EIF2B), which is necessary for protein synthesis, is a GTP exchange factor composed of five different subunits. The protein encoded by this gene is the fourth, or delta, subunit. Defects in this gene are a cause of leukoencephalopathy with vanishing white matter (VWM) and ovarioleukodystrophy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence and uses an alternate in-frame splice junction compared to variant 4. The resulting isoform (3) has a shorter and distinct N-terminus and lacks an internal aa compared to isoform 4.