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DBF4 (NM_006716) Human Untagged Clone

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SKU
SC108298
DBF4 (untagged)-Human DBF4 homolog (S. cerevisiae) (DBF4)
  $618.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol DBF4
Synonyms ASK; CHIF; DBF4A; ZDBF1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC108298 sequence for NM_006716 edited (data generated by NextGen Sequencing)
ATGAACTCCGGAGCCATGAGGATCCACAGTAAAGGACATTTCCAGGGTGGAATCCAAGTC
AAAAATGAAAAAAACAGACCATCTCTGAAATCTCTGAAAACTGATAACAGGCCAGAAAAA
TCCAAATGTAAGCCACTTTGGGGAAAAGTATTTTACCTTGACTTACCTTCTGTCACCATA
TCTGAAAAACTTCAAAAGGACATTAAGGATCTGGGAGGGCGAGTTGAAGAATTTCTCAGC
AAAGATATCAGTTATCTTATTTCAAATAAGAAGGAAGCTAAATTTGCACAAACCTTGGGT
CGAATTTCTCCTGTACCAAGTCCAGAATCTGCATATACTGCAGAAACCACTTCACCTCAT
CCCAGCCATGATGGAAGTTCATTTAAGTCACCAGACACAGTSTGTTTAAGCAGAGGAAAA
TTATTAGTTGAAAAAGCTATCAAGGACCATGATTTTATTCCTTCAAATAGTATATTATCA
AATGCCTTGTCATGGGGAGTAAAAATTCTTCATATTGATGACATTAGATACTACATTGAA
CAAAAGAAAAAAGAGTTGTATTTACTCAAGAAATCAAGTACTTCAGTAAGAGATGGGGGC
AAAAGAGTTGGTAGTGGTGCACAAAAAACAAGAACAGGAAGACTCAAAAAGCCTTTTGTA
AAGGTGGAAGATATGAGCCAACTTTATAGGCCATTTTATCTTCAGCTGACCAATATGCCT
TTTATAAATTATTCTATTCAGAAGCCCTGCAGTCCATTTGATGTAGACAAGCCATCTAGT
ATGCAAAAGCAAACTCAGGTTAAACTAAGAATCCAAACAGATGGCGATAAGTATGGTGGA
ACCTCAATTCAACTCCAGTTGAAAGAGAAGAAGAAAAAAGGATATTGTGAATGTTGCTTG
CAGAAATATGAAGATCTAGAAACTCACCTTCTAAGTGAGCAACACAGAAACTTTGCACAG
AGTAACCAGTATCAAGTTGTTGATGATATTGTATCTAAGTTAGTTTTTGACTTTGTGGAA
TATGAAAAGGACACACCTAAAAAGAAAAGAATAAAATACAGTGTTGGATCCCTTTCTCCT
GTTTCTGCAAGTGTCCTGAAAAAGACTGAACAAAAGGAAAAAGTGGAATTGCAACATATT
TCTCAGAAAGATTGCCAGGAAGATGATACAACAGTGAAGGAGCAGAATTTCCTGTATAAA
GAGACCCAGGAAACTGAAAAAAAGCTCCTGTTTATTTCAGAGCCCATCCCCCACCCTTCA
AATGAATTGAGAGGGCTTAATGAGAAAATGAGTAATAAATGTTCCATGTTAAGTACAGCT
GAAGATGACATAAGACAGAATTTTACACAGCTACCTCTACATAAAAACAAACAGGAATGC
ATTCTTGACATTTCCGAACACACATTAAGTGAAAATGACTTAGAAGAACTAAGGGTAGAT
CACTATAAATGTAACATACAGGCATCTGTACATGTTTCTGATTTCAGTACAGATAATAGT
GGATCTCAACCAAAACAGAAGTCAGATACTGTGCTTTTTCCAGCAAAGGATCTCAAGGAA
AAGGACCTTCATTCAATATTTACTCATGATTCTGGTCTGATAACAATAAACAGTTCACAA
GAGCACCTAACTGTTCAGGCAAAGGCTCCATTCCATACTCCTCCTGAGGAACCCAATGAA
TGTGACTTCAAGAATATGGATAGTTTACCTTCTGGTAAAATACATCGAAAAGTGAAAATA
ATATTAGGACGAAATAGAAAAGAAAATCTGGAACCAAATGCTGAATTTGATAAAAGAACT
GAATTTATTACACAAGAAGAAAACAGAATTTGTAGTTCACCGGTACAGTCTTTACTAGAC
TTGTTTCAGACTAGTGAAGAGAAATCAGAATTTTTGGGTTTCACAAGCTACACAGAAAAG
AGTGGTATATGCAATGTTTTAGATATTTGGGAAGAGGAAAATTCAGATAATCTGTTAACA
GCGTTTTTCTCGTCCCCTTCAACTTCTACATTTACTGGCTTTTAG

Clone variation with respect to NM_006716.3
402 g=>s
5' Read Nucleotide Sequence
>OriGene 5' read for NM_006716 unedited
TTGGTCAGAATTTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGAAAGAGAG
AGGCGGCCGTCCTGTCAACAGGCCGGGGGAAGCCGTGCTTTCGCGGCTGCCCGGTGCGAC
ACTTTCTCCGGACCCAGCATGTAGGTGCCGGGCGACTGCCATGAACTCCGGAGCCATGAG
GATCCACAGTAAAGGACATTTCCAGGGTGGAATCCAAGTCAAAAATGAAAAAAACAGACC
ATCTCTGAAATCTCTGAAAACTGATAACAGGCCAGAAAAATCCAAATGTAAGCCACTTTG
GGGAAAAGTATTTTACCTTGACTTACCTTCTGTCACCATATCTGAAAAACTTCAAAAGGA
CATTAAGGATCTGGGAGGGCGAGTTGAAGAATTTCTCAGCAAAGATATCAGTTATCTTAT
TTCAAATAAGAAGGAAGCTAAATTTGCACAAACCTTGGGTCGAATTTCTCCTGTACCAAG
TCCAGAATCTGCATATACTGCAGAAACCACTTCACCTCATCCCAGCCATGATGGAAGTTC
ATTTAAGTCACCAGACACAGTCTTTTTATAGGTGTGTTTAAGCAGAGGAAAATTATTAGT
TGAAAAAGCTATCAAGGACCATGATTTTATTCCTTCAAATAGTATATTATCAAATGCCTT
GTCATGGGGAGTAAAAATTCTTCATATTGATGACATTAGATACTACATTGAACAAAAGAA
AAAAGAGTTGTATTTACTCAAGAAATCAAGTACTTCAGTAAGAGATGGGGGCAAAAGAGT
TGGTAGTGGTGCACAAAANNACAGAACAGGAAGACTCANAAAGCCTTTTGTAANGGTGGA
AGATATGAGCCCACTTATAGGGCCATTTATCTTCAGCTGACCATATGCCTTTATNAANTA
TNCTATCAGAAGCCTGCAGTCATNTGATGTGAA
3' Read Nucleotide Sequence
>OriGene 3' read for NM_006716 unedited
GGCCCTACTGTGNACCGCGGNCCGCTATTTNANGATCGNGTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTAATGTAAAACAAAACCAGTCACAAGTTTATTCTGTAATTTTTCTTTAACAG
GTATTCAATTCTGTAAAAAATTGCAAATATTTATTTTTAATATTTACATTTGGGTCTGTA
AACAAAGCTGGTAAAAAAATCCTAAAAATTTCCATACATATTTATAAAAATTTCAAGAAT
ATGATCCTTATCACTTCTGAAAAGTATGCATTTTTTAAATTCTAAAAGCCAGTAAATGTA
AAAGTTGAAGGGGACGAAAAAAACGCTGTTAACAGATTATCTGAATTTTCCTCTTCCCAA
ATATCTAAAACATTGCATATACCACTCTTTTCTGTGTAGCTTGTGAAACCCAAAAATTCT
GATTTCTCTTCACTAGTCTGAAACAAGTCTAGTAAAGACTGTACCGGTGAACTACAAATT
CTGTTTTCTTCTTGTGTAATAAATTCAGTTCTTTTATCAAATTCAGCATTTGGTTCCAGA
TTTTCTTTTCTATTTCGTCCTAATATTATTTTCACTTTTCGATGTATTTTACCAGAAGGT
AAACTATCCATATTCTTGAAGTCACATTCATTGGGTTCCTCAGGAGGAGTATGGAATGGA
GCCTTTGCCTGAACAGTTAGGTGCTCTTGTGAACTGTTTATTGTTATCAGACCAGAATCA
TGAGTAAATATTGAATGAAGGTCCTTTTCCTTGAGATCCTTTGCTGGAAAAACACAGTAT
CTGACTTCTGTTTTGGTTGAGATCCACTATTATCTGTACTGAAATCAGNACATGTACAGA
TGCCTGTATGTTACATTTATAGNGATCTACCCTTAGNTNCTTCTAGNNCATTNTCACTTA
ATGNGNNGTCCGAAAATGTCAAGATGCATTCCTGNTTGTGTTTATGG
Restriction Sites NotI-NotI |
ACCN NM_006716
Insert Size 2710 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006716.3, NP_006707.1
RefSeq Size 3923 bp
RefSeq ORF 2025 bp
Locus ID 10926
UniProt ID Q9UBU7
Cytogenetics 7q21.12
Domains ZnF_DBF
Protein Families Druggable Genome
Protein Pathways Cell cycle
Summary Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).
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Documents
Resources
"NM_006716" in other vectors (6)
SKU Description Size Price
RC206120 DBF4 (Myc-DDK-tagged)-Human DBF4 homolog (S. cerevisiae) (DBF4) 10 ug
$617.00
RC206120L1 Lenti ORF clone of Human DBF4 homolog (S. cerevisiae) (DBF4), Myc-DDK-tagged 10 ug
$917.00
RC206120L2 Lenti ORF clone of Human DBF4 homolog (S. cerevisiae) (DBF4), mGFP tagged 10 ug
$917.00
RC206120L3 Lenti ORF clone of Human DBF4 homolog (S. cerevisiae) (DBF4), Myc-DDK-tagged 10 ug
$917.00
RC206120L4 Lenti ORF clone of Human DBF4 homolog (S. cerevisiae) (DBF4), mGFP tagged 10 ug
$917.00
RG206120 DBF4 (tGFP-tagged) - Human DBF4 homolog (S. cerevisiae) (DBF4) 10 ug
$789.00 $817.00

file-alt Citations

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