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EED (NM_003797) Human Untagged Clone

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SKU
SC108260
EED (untagged)-Human embryonic ectoderm development (EED), transcript variant 1
  $457.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol EED
Synonyms COGIS; HEED; WAIT1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC108260 sequence for NM_003797 edited (data generated by NextGen Sequencing)
ATGTCCGAGAGGGAAGTGTCGACTGCGCCGGCGGGAACAGACATGCCTGCGGCCAAGAAG
CAGAAGCTGAGCAGTGACGAGAACAGCAATCCAGACCTCTCTGGAGACGAGAATGATGAC
GCTGTCAGTATAGAAAGTGGTACAAACACTGAACGCCCTGATACACCTACAAACACGCCA
AATGCACCTGGAAGGAAAAGTTGGGGAAAGGGAAAATGGAAGTCAAAGAAATGCAAATAT
TCTTTCAAATGTGTAAATAGTCTCAAGGAAGATCATAACCAACCATTGTTTGGAGTTCAG
TTTAACTGGCACAGTAAAGAAGGAGATCCATTAGTGTTTGCAACTGTAGGAAGCAACAGA
GTTACCTTGTATGAATGTCATTCACAAGGAGAAATCCGGTTGTTGCAATCTTACGTGGAT
GCTGATGCTGATGAAAACTTTTACACTTGTGCATGGACCTATGATAGCAATACGAGCCAT
CCTCTGCTGGCTGTAGCTGGATCTAGAGGCATAATTAGGATAATAAATCCTATAACAATG
CAGTGTATAAAGCACTATGTTGGCCATGGAAATGCTATCAATGAGCTGAAATTCCATCCA
AGAGATCCAAATCTTCTCCTGTCAGTAAGTAAAGATCATGCTTTACGATTATGGAATATC
CAGACGGACACTCTGGTGGCAATATTTGGAGGCGTAGAAGGGCACAGAGATGAAGTTCTA
AGTGCTGATTATGATCTTTTGGGTGAAAAAATAATGTCCTGTGGTATGGATCATTCTCTT
AAACTTTGGAGGATCAATTCAAAGAGAATGATGAATGCAATTAAGGAATCTTATGATTAT
AATCCAAATAAAACTAACAGGCCATTTATTTCTCAGAAAATCCATTTTCCTGATTTTTCT
ACCAGAGACATACATAGGAATTATGTTGATTGTGTGCGATGGTTAGGCGATTTGATACTT
TCTAAGTCTTGTGAAAATGCCATTGTGTGCTGGAAACCTGGCAAGATGGAAGATGATATA
GATAAAATTAAACCCAGTGAATCTAATGTGACTATTCTTGGGCGATTTGATTACAGCCAG
TGTGACATTTGGTACATGAGGTTTTCTATGGATTTCTGGCAAAAGATGCTTGCATTGGGC
AATCAAGTTGGCAAACTTTATGTTTGGGATTTAGAAGTAGAAGATCCTCATAAAGCCAAA
TGTACAACACTGACTCATCATAAATGTGGTGCTGCTATTCGACAAACCAGTTTTAGCAGG
GATAGCAGCATTCTTATAGCTGTTTGTGATGATGCCAGTATTTGGCGCTGGGATCGACTT
CGATAA

Clone variation with respect to NM_003797.3
5' Read Nucleotide Sequence
>OriGene 5' read for NM_003797 unedited
AAACGCAATTCGCACGAGCCTCGTGCCGAATTCGGCACGAGGGTCGCACGCACGCCCGCC
TCGGCGGCTGGGCGCGATTTGCGACAGTGGGGGGGGCGGTGGAGGTGGCGGCGGCAGCGG
CAACTTTGCGGCAAGCTCGGGCCGGGCTTGCTTGACGGCGGTGTGGCGGAGGCCCCGCCT
TATGCGGCAGGAACCTGGAGGGAGGCGGAGGAATATGTCCGAGAGGGAAGTGTCGACTGC
GCCGGCGGGAACAGACATGCCTGCGGCCAAGAAGCAGAAGCTGAGCAGTGACGAGAACAG
CAATCCAGACCTCTCTGGAGACGAGAATGATGACGCTGTCAGTATAGAAAGTGGTACAAA
CACTGAACGCCCTGATACACCTACAAACACGCCAAATGCACCTGGAAGGAAAAGTTGGGG
AAAGGGAAAATGGAAGTCAAAGAAATGCAAATATTCTTTCAAATGTGTAAATAGTCTCAA
GGAAGATCATAACCAACCATTGTTTGGAGTTCAGTTTAACTGGCACAGTAAAGAAGGAGA
TCCATTAGTGTTTGCAACTGTAGGAAGCAACAGAGTTACCTTGTATGAATGTCATTCACA
AGGAGAAATCCGGTTGTTGCAATCTTACGTGGATGCTGATGCTGATGAAAACTTTTACAC
TTGTGCATGGACCTATGATAGCAATACGAGCCATCCTCTGCTGGCTGTAGCTGGATCTAN
GAGCATAATTANGATAATAAATCCTATAACAATGCAGTGTATAAAGCACTATGTTGGCCA
TGGAAATGCTATCAATGAGCTGAAATTCCATCCAAGAGATCCAAATCTNCTNCTGTCAGT
AAGTAAAGATTCATGCTTTACGATTATGGAATATTCAGACGGACACTCTGGC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_003797 unedited
CGCGGCCGCAATCTAAAGTCGAGTTTTTTTTTTTTTTTTTTACAAAAATAAATTTTTATT
AAAAGCAGTAGAGTCTGAGCAGGAAGACAGTACAAAGAATGTAAACAATGTAAACATCAC
TACATTCAGCTCAGCCTGATTGAATGCTGAAAGACAACTCTAAATGCTCTACGTGCCCTT
ACTAGCAAGATACATTAATTCTATTTTACACAGACAACAAACACACTCTAATTTTGATTA
GGCAAAAGTATTTTATCGAAGTCGATCCCAGCGCCAAATACTGGCATCATCACAAACAGC
TATAAGAATGCTGCTATCCCTGCTAAAACTGGTTTGTCGAATAGCAGCACCACATTTATG
ATGAGTCAGTGTTGTACATTTGGCTTTATGAGGATCTTCTACTTCTAAATCCCAAACATA
AAGTTTGCCAACTTGATTGCCCAATGCAAGCATCTTTTGCCAGAAATCCATAGAAAACCT
CATGTACCAAATGTCACACTGGCTGTAATCAAATCGCCCAAGAATAGTCACATTAGATTC
ACTGGGTTTAATTTTATCTATATCATCTTCCATCTTGCCAGGTTTCCAGCACACAATGGC
ATTTTCACAAGACTTAGAAAGTATCAAATCGCCTAACCATCGCACACAATCAACATAATT
CCTATGTATGTCTCTGGTAGAAAATCAGGAAAATGGACTTTCTGAGAAAAAATGGCCTGT
TAGTNTTATTTGGGATATAATCATAAGAATCCTTAATNGCATTCATCATTCTTTTTGAAT
CGATCCTCCCAGGTTTAGAAGATGATCCATCCACCGGACATTTTTTTTCCACCAAAAGAC
ATAATCAGCCTTAAAACTCTCCTTGGGCCCTTTACGCTCCAAATTTGCCCCGAAGGCCGT
TTGTAATTCATAACGGAAACAGAACTTCCTACTGCCGAGAAAATTGACCTTGGTGGAATT
CACTATGAAGCCTCCCTGGCAAAAAGCGTTACCCGATGGTTAGATAATCCCT
Restriction Sites NotI-NotI |
ACCN NM_003797
Insert Size 1690 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003797.2, NP_003788.2
RefSeq Size 2006 bp
RefSeq ORF 1326 bp
Locus ID 8726
UniProt ID O75530
Cytogenetics 11q14.2
Domains WD40
Protein Families Druggable Genome, Transcription Factors
Summary This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. Two transcript variants encoding distinct isoforms have been identified for this gene. provided by RefSeq, Jul 2008
Transcript Variant: This variant (1) lacks an in-frame exon in the 3' coding region compared to variant 3. It encodes isoform a, which is shorter than isoform c.
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Documents
Resources
"NM_003797" in other vectors (6)
SKU Description Size Price
RC219261 EED (Myc-DDK-tagged)-Human embryonic ectoderm development (EED), transcript variant 1 10 ug
$457.00
RC219261L1 Lenti ORF clone of Human embryonic ectoderm development (EED), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC219261L2 Lenti ORF clone of Human embryonic ectoderm development (EED), transcript variant 1, mGFP tagged 10 ug
$757.00
RC219261L3 Lenti ORF clone of Human embryonic ectoderm development (EED), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC219261L4 Lenti ORF clone of Human embryonic ectoderm development (EED), transcript variant 1, mGFP tagged 10 ug
$757.00
RG219261 EED (tGFP-tagged) - Human embryonic ectoderm development (EED), transcript variant 1 10 ug
$489.00 $657.00

file-alt Citations

Bromodomain protein 7 interacts with PRMT5 and PRC2, and is involved in transcriptional repression of their target genes
Tae, Karkhanis, Velasco et al
Nucleic Acids Res. (2011) 39 (13), 5424-38 DOI: 10.1093/nar/gkr170
Cited by 92
AI Summary: OriGene’s pCMV6-XL5/BRD7 vector was used as the source of BRD7 cDNA for cloning and protein expression in this study, enabling functional analyses of BRD7’s role in transcriptional repression.
Snippet: a 1.9-kb BRD7 cDNA-encoding amino acids 2–651, which was PCR-amplified from the pCMV6-XL5/BRD7 vector (Origene Technologies Inc ... Plasmid pGEX-2TK/BRD7 (amino acids 2–651), which expresses GST–BRD7 used in GST pull-down assays and antibody production
FigureFigureFigure
Showing 1-1 of 1 papers.
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