OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

ITCH (NM_031483) Human Untagged Clone

Be the first to review this product
SKU
SC108233
ITCH (untagged)-Human itchy E3 ubiquitin protein ligase homolog (mouse) (ITCH)
  $790.00
4 Weeks*
Bulk Requests & Clone Modifications

Frequently Bought Together (4)

TA377593 ITCH Rabbit polyclonal Antibody
100 ul
$475.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
TF81001 TurboFectin Transfection Reagent (1 mL in 1 vial)
1 ml in 1 vial
$530.00

Other products for "ITCH or NM_031483"

Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol ITCH
Synonyms ADMFD; AIF4; AIP4; NAPP1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_031483, the custom clone sequence may differ by one or more nucleotides


ATGTCTGACAGTGGATCACAACTTGGTTCAATGGGTAGCCTCACCATGAAATCACAGCTTCAGATCACTG
TCATCTCAGCAAAACTTAAGGAAAATAAGAAGAATTGGTTTGGACCAAGTCCTTACGTAGAGGTCACAGT
AGATGGACAGTCAAAGAAGACAGAAAAATGCAACAACACAAACAGTCCCAAGTGGAAGCAACCCCTTACA
GTTATCGTTACCCCTGTGAGTAAATTACATTTTCGTGTGTGGAGTCACCAGACACTGAAATCTGATGTTT
TGTTGGGAACTGCTGCATTAGATATTTATGAAACATTAAAGTCAAACAATATGAAACTTGAAGAAGTAGT
TGTGACTTTGCAGCTTGGAGGTGACAAAGAGCCAACAGAGACAATAGGAGACTTGTCAATTTGTCTTGAT
GGGCTACAGTTAGAGTCTGAAGTTGTTACCAATGGTGAAACTACATGTTCAGAAAGTGCTTCTCAGAATG
ATGATGGCTCCAGATCCAAGGATGAAACAAGAGTGAGCACAAATGGATCAGATGACCCTGAAGATGCAGG
AGCTGGTGAAAATAGGAGAGTCAGTGGGAATAATTCTCCATCACTCTCAAATGGTGGTTTTAAACCTTCT
AGACCTCCAAGACCTTCACGACCACCACCACCCACCCCACGTAGACCAGCATCTGTCAATGGTTCACCAT
CTGCCACTTCTGAAAGTGATGGGTCTAGTACAGGCTCTCTGCCGCCGACAAATACAAATACAAATACATC
TGAAGGAGCAACATCTGGATTAATAATTCCTCTTACTATATCTGGAGGCTCAGGCCCTAGGCCATTAAAT
CCTGTAACTCAAGCTCCCTTGCCACCTGGTTGGGAGCAGAGAGTGGACCAGCACGGGCGAGTTTACTATG
TAGATCATGTTGAGAAAAGAACAACATGGGATAGACCAGAACCTCTACCTCCTGGCTGGGAACGGCGGGT
TGACAACATGGGACGTATTTATTATGTTGACCATTTCACAAGAACAACAACGTGGCAGAGGCCAACACTG
GAATCCGTCCGGAACTATGAACAATGGCAGCTACAGCGTAGTCAGCTTCAAGGAGCAATGCAGCAGTTTA
ACCAGAGATTCATTTATGGGAATCAAGATTTATTTGCTACATCACAAAGTAAAGAATTTGATCCTCTTGG
TCCATTGCCACCTGGATGGGAGAAGAGAACAGACAGCAATGGCAGAGTATATTTCGTCAACCACAACACA
CGAATTACACAATGGGAAGACCCCAGAAGTCAAGGTCAATTAAATGAAAAGCCCTTACCTGAAGGTTGGG
AAATGAGATTCACAGTGGATGGAATTCCATATTTTGTGGACCACAATAGAAGAACTACCACCTATATAGA
TCCCCGCACAGGAAAATCTGCCCTAGACAATGGACCTCAGATAGCCTATGTTCGGGACTTCAAAGCAAAG
GTTCAGTATTTCCGGTTCTGGTGTCAGCAACTGGCCATGCCACAGCACATAAAGATTACAGTGACAAGAA
AAACATTGTTTGAGGATTCCTTTCAACAGATAATGAGCTTCAGTCCCCAAGATCTGCGAAGACGTTTGTG
GGTGATTTTTCCAGGAGAAGAAGGTTTAGATTATGGAGGTGTAGCAAGAGAATGGTTCTTTCTTTTGTCA
CATGAAGTGTTGAACCCAATGTATTGCCTGTTTGAATATGCAGGGAAGGATAACTACTGCTTGCAGATAA
ACCCCGCTTCTTACATCAATCCAGATCACCTGAAATATTTTCGTTTTATTGGCAGATTTATTGCCATGGC
TCTGTTCCATGGGAAATTCATAGACACGGGTTTTTCTTTACCATTCTATAAGCGTATCTTGAACAAACCA
GTTGGACTCAAGGATTTAGAATCTATTGATCCAGAATTTTACAATTCTCTCATCTGGGTTAAGGAAAACA
ATATTGAGGAATGTGATTTGGAAATGTACTTCTCCGTTGACAAAGAAATTCTAGGTGAAATTAAGAGTCA
TGATCTGAAACCTAATGGTGGCAATATTCTTGTAACAGAAGAAAATAAAGAGGAATACATCAGAATGGTA
GCTGAGTGGAGGTTGTCTCGAGGTGTTGAAGAACAGACACAAGCTTTCTTTGAAGGCTTTAATGAAATTC
TTCCCCAGCAATATTTGCAATACTTTGATGCAAAGGAATTAGAGGTCCTTTTATGTGGAATGCAAGAGAT
TGATTTGAATGACTGGCAAAGACATGCCATCTACCGTCATTATGCAAGGACCAGCAAACAAATCATGTGG
TTTTGGCAGTTTGTTAAAGAAATTGATAATGAGAAGAGAATGAGACTTCTGCAGTTTGTTACTGGAACCT
GCCGATTGCCAGTAGGAGGATTTGCTGATCTCATGGGGAGCAATGGACCACAGAAATTCTGCATTGAAAA
AGTTGGGAAAGAAAATTGGCTACCCAGAAGTCATACCTGTTTTAATCGCCTGGACCTGCCACCATACAAG
AGCTATGAGCAACTGAAGGAAAAGCTGTTGTTTGCCATAGAAGAAACAGAAGGATTTGGACAAGAGTAA

5' Read Nucleotide Sequence
>OriGene 5' read for NM_031483 unedited
GGGCACATTTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGATGCATTTCA
CAGTGGCCTTGTGGAGACAACGCCTTAACCCAAGGAAGTGACTCAAACTGTGAGAACTTC
AGGTTTTCCAACCTATTGGTGGTATGTCTGACAGTGGATCACAACTTGGTTCAATGGGTA
GCCTCACCATGAAATCACAGCTTCAGATCACTGTCATCTCAGCAAAACTTAAGGAAAATA
AGAAGAATTGGTTTGGACCAAGTCCTTACGTAGAGGTCACAGTAGATGGACAGTCAAAGA
AGACAGAAAAATGCAACAACACAAACAGTCCCAAGTGGAAGCAACCCCTTACAGTTATCG
TTACCCCTGTGAGTAAATTACATTTTCGTGTGTGGAGTCACCAGACACTGAAATCTGATG
TTTTGTTGGGAACTGCTGCATTAGATATTTATGAAACATTAAAGTCAAACAATATGAAAC
TTGAAGAAGTAGTTGTGACTTTGCAGCTTGGAGGTGACAAAGAGCCAACAGAGACAATAG
GAGACTTGTCAATTTGTCTTGATGGGCTACAGTTAGAGTCTGAAGTTGTTACCAATGGTG
AAACTACATGTTCAGAAAGTGCTTCTCAGAATGATGATGGCTCCAGATCCAAGGATGAAA
CAAGAGTGAGCACAAATGGATCAGATGACCCTGAAGATGCAAGAGCTGGTGAAAATAGGA
GAGTCAGTGGGAATAATTCTCCATCACTCTCAAATGGTGGTTTTAAACCTCTAGACCTCA
AGACCTTCACGACCACCACCACCCACCCCACGTAGACCAGCATCTGTCAATGGGTCACCA
TCCTGCCACTTTGAAAGTGAGGGTCTAGTACAAGCTCTCCTGCCCCGACAAAACCAATAC
AATACATCTGG
3' Read Nucleotide Sequence
>OriGene 3' read for NM_031483 unedited
GCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTATTTATCCCCCACCACGCTGCCC
AAAAAAAAGAACAGGGTCTCCCTATGTTGCCCAGGCTGGTCTTTAACTCCTGGGCTAAAG
GGATCCTCCTGCCTCGGCCTCCCAGAGTGCTAGGATTACAGGTATGAGCCACCATGCCCG
GCCCTAGGTTAACTTCTTAAAACAATGAAGGAATCACAGTGTTAACATTGTTTGTAAAGT
GTAGTATTTGTCAGGCAACTGAAACAATCAGACTTCCAAATGAAGTCATTTGCAGGCTGT
GACCAACTTTTCCCTGAATTCAAATTGACAAAACTACTTTGTAGAGATGCAAGAGTCTTG
CCTCACTTCCAGGAATGATGTATGGATTCATGCGCCAGAAAGGAAAAACAATAGTCAAGT
CAGACTAAAATGAGAGGGAGAACACAAGCCCGAATTTTGCTCACATCCNCAGAAGCAGGA
GTAAGCTTGCACTTTTAACAAATGCCTCTTACCAAGAGGCTTAATAGGAAAATCTTGTAT
ATTGCAGTTACCAAAGGACTTCCCTGCATGTCTGCAGAATGTCTAANAGAAAAAACACTA
CTCCCAAGTGAGACTTTTGACACACATAANATATTGTGTTAAAGGAATAACTAGTGGAAT
AGACAAGGGCAATTCATTGTGTGTAAAATCTATGTTAGCAGGTTCTGACTAGTCATTGCA
GACCTGATTCTGGAACGCTGAGTGAAATCACTGGAGATAATCTAATGAGACATTATTTAC
TTCACTCAGATACTCTCTAAGACCCATTGTCATTTACAGATGTGCCACAGCANAAAAGAC
AAACATGCAATTAGTCCTGCCATTCATGTGCCAGTTTAAAGTACTCTGCCAATCCTCTGT
TTTCTATGCAACAAAGCTTNCTAGTGCTAAACTCTGGTGGGGCAGTCAGCGATAAACGGT
GACTCTGGACCATTTCTTCCACTTTCAGCAATTCTTGGCATGCTCCAGAAAACAACTC
Restriction Sites NotI-NotI |
ACCN NM_031483
Insert Size 3910 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_031483.3, NP_113671.3
RefSeq Size 6357 bp
RefSeq ORF 2589 bp
Locus ID 83737
UniProt ID Q96J02
Cytogenetics 20q11.22
Domains C2, HECT, WW
Protein Families Druggable Genome, Transcription Factors
Protein Pathways Endocytosis, Ubiquitin mediated proteolysis
Summary This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. provided by RefSeq, Mar 2012
Transcript Variant: This variant (2) lacks an exon in the 5' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Both variants 2 and 5 encode the same isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_031483" in other vectors (6)
SKU Description Size Price
RC220408 ITCH (Myc-DDK-tagged)-Human itchy E3 ubiquitin protein ligase homolog (mouse) (ITCH) 10 ug
$789.00
RC220408L1 Lenti ORF clone of Human itchy E3 ubiquitin protein ligase homolog (mouse) (ITCH), Myc-DDK-tagged 10 ug
$1,089.00
RC220408L2 Lenti ORF clone of Human itchy E3 ubiquitin protein ligase homolog (mouse) (ITCH), mGFP tagged 10 ug
$1,089.00
RC220408L3 Lenti ORF clone of Human itchy E3 ubiquitin protein ligase homolog (mouse) (ITCH), Myc-DDK-tagged 10 ug
$1,089.00
RC220408L4 Lenti ORF clone of Human itchy E3 ubiquitin protein ligase homolog (mouse) (ITCH), mGFP tagged 10 ug
$1,089.00
RG220408 ITCH (tGFP-tagged) - Human itchy E3 ubiquitin protein ligase homolog (mouse) (ITCH) 10 ug
$789.00 $989.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products