PRMT8 (NM_019854) Human Untagged Clone

SKU SC108108

PRMT8 (untagged)-Human protein arginine methyltransferase 8 (PRMT8)

Product Data

• Type: Human Untagged Clone
• Target Symbol: PRMT8
• Synonyms: HRMT1L3; HRMT1L4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108108 sequence for NM_019854 edited (data generated by NextGen Sequencing); ATGGGCATGAAACACTCCTCCCGCTGCCTGCTCCTGAGGAGGAAAATGGCGGAGAACGCG; GCCGAGAGCACCGAGGTGAACAGCCCCCCCTCCCAGCCCCCCCAGCCCGTCGTCCCTGCT; AAGCCCGTGCAATGCGTCCATCATGTGTCCACTCAACCCAGCTGCCCAGGACGGGGCAAG; ATGTCCAAGCTGCTGAACCCAGAGGAGATGACCTCGAGAGATTATTACTTCGACTCCTAT; GCCCACTTTGGGATCCACGAGGAAATGCTGAAGGATGAGGTGCGGACTCTCACTTACCGG; AACTCCATGTACCACAACAAGCACGTGTTCAAGGACAAAGTGGTACTGGATGTGGGGAGT; GGTACTGGGATCCTTTCCATGTTCGCTGCCAAGGCAGGGGCCAAGAAGGTGTTTGGGATC; GAATGCTCCAGTATTTCTGACTACTCAGAGAAGATCATTAAGGCCAACCACTTGGACAAC; ATCATCACCATATTTAAGGGTAAAGTGGAAGAGGTGGAGCTGCCTGTGGAGAAGGTGGAC; ATCATCATCAGCGAGTGGATGGGCTACTGTCTGTTCTATGAGTCCATGCTCAACACGGTG; ATCTTTGCCAGGGACAAGTGGCTGAAACCTGGAGGGCTTATGTTTCCAGACCGGGCAGCT; TTGTACGTGGTAGCGATTGAAGACAGACAGTACAAGGACTTCAAAATCCACTGGTGGGAG; AATGTCTATGGCTTTGACATGACCTGCATCCGGGACGTGGCCATGAAGGAGCCTCTAGTG; GACATCGTGGATCCAAAGCAAGTGGTGACCAATGCCTGTTTGATAAAGGAGGTGGACATT; TACACAGTGAAGACGGAAGAGCTATCGTTCACATCTGCATTCTGCCTGCAGATACAGCGC; AACGACTACGTCCACGCCCTGGTCACCTATTTTAATATTGAATTTACCAAGTGCCACAAG; AAAATGGGGTTTTCCACAGCCCCTGATGCTCCCTACACCCACTGGAAGCAGACCGTCTTC; TACTTGGAAGATTACCTCACTGTCCGAAGGGGGGAGGAAATCTACGGGACCATATCCATG; AAGCCAAATGCCAAAAATGTGCGAGACCTCGATTTCACAGTAGACTTGGATTTTAAGGGA; CAGCTGTGTGAAACATCTGTATCTAATGACTACAAAATGCGTTAG; ; Clone variation with respect to NM_019854.3; 1047 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_019854 unedited; NGTCAGAATTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACGAGGCCGCCGCCG; CCGCCGCCGCCGCGGAGGCTTCGGGGCTGCTTCCCTCGAGCTTAGCCCGCAGCGCGGGTG; GAGAGGGGCGGGGAGGGGGTCGGGGGCACGAGAAGAACTTGAAACCGTGTGAAGGAATCC; GGAGCAGATGAGAAGGGAGGAAAATAAAAGAAAGTGGAGACTGCAGAACAGACTCCGCTG; TGGCTGACTGTGCCGGCCGACGCTCCAGCTGAGGGGCTGGGTTGGATTTTTTTTTTTCTC; CCATCCTCTCGCTCTCTCTTTTAAAGCGACACCAGCTCTCTCTCCTCCTCTACTATCTCG; GTATCACCAAACCCTTGCCGGCTCTTATGGGCATGAAACACTCCTCCCGCTGCCTGCTCC; TGAGGAGGAAAATGGCGGAGAACGCGGCCGAGAGCACCGAGGTGAACAGCCCCCCCTCCC; AGCCCCCCCAGCCCGTCGTCCCTGCTAAGCCCGTGCAATGCGTCCATCATGTGTCCACTC; AACCCAGCTGCCCAGGACGGGGCAAGATGTCCAAGCTGCTGAACCCAGAGGAGATGACCT; CGAGAGATTATTACTTCGACTCCTATGCCCACTTTGGGATCCACGAGGAAATGCTGAAGG; ATGAGGTGCGGACTCTCACTTACCGGAACTCCATGTACCACAACAAGCACGTGTTCAAGG; ACAAAGTGGTACTGGATGTGGCGAGTGGTACTGGGATCCTTTCCTGTTCGCTGCCAGGCA; GGGCCAAGAAGGTGTTTGGGATCGATGCTCCAGTATTCTGACTACTCANAGAGATCATTA; GGCCACCACTTGGACACATCACACCATATTAAGGTTAAGTGGAACAAGTGGACCTGCTA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_019854 unedited; CTATGGACCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGTTCTCATGT; GTGTTTATTGAGCGCCCCGGAGTCCGTGGTACTATGCTCAAGGTTCAGAGCCACAGTCCA; GTCAAAAGGAGACCAGAACTAGTACAGAGAATGTTTCATAGCTGCTTAACATCTGGTAAG; GGGTGGGGAAGGGGCGAACTAATCACAAAACAAATTCCTATTCAAAAGCAACAGAATCTC; CTCTGAGAGTAATATATATTCTAGGCACACGCGCACGCACACACACACACACCCCTTTGA; GAGAACACCTAGGTCTACCCACTTGGTTGTATATGCACGCATATACAGGTGTTTGTGGGT; AAATATCAACTACGCTGCTCACATTCACACATGCACTCACACGCACACCACCGGACACAC; ACATAATCTGGCTTATGCTATCAAAGATGCTATGTCTCCAGGTTCTAGTGGAAGCAATCT; GTCCTAGACACCCGCAGCATGCAAAACTCAGAAGACCCGGAGAGTCACAGTTAAGGAGGA; GACGGGGAGACGCGAATACGTTTCCACCCTCGGGGCCCAGCCACAGCACGCCAGGTCGCT; CTTTGTTGGCAACACAAAGCCTTCGTGAAGGGCTACCAGGGCAGAGCGGAGGAGCTGGAG; GCCTTCTGTCCAGTGGCAGAGCCCACGGGAGCCCTGNGGAGTTCACCAATCTTCAAGGCA; GAGTTGAAAACTCTGGTTTTCAAGTGTGTAGTCCTGCAAACGGTATTCTTTGGGACGGCA; CGGCAGATCGAGGTGAGAAGTCCTTTTCTCGTTGCTCTCTGCAGCTTCCCACGTGTGCTA; ACGCATTTTGTAGTCATTAGATACAGATGTTTCACACAGCTGTCCCTTAAAATCCAATCT; ACTGTGAAATCCAGGTCTCGCACATTTTTGCATTTGGCTTCATGGATTGGCCCCGAAAAT; TCCTCCCCCTTCGACAGTGAG;
• Restriction Sites: NotI-NotI
• ACCN: NM_019854
• Insert Size: 2560 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_019854.3, NP_062828.3
• RefSeq Size: 2356 bp
• RefSeq ORF: 1185 bp
• Locus ID: 56341
• UniProt ID: Q9NR22
• Cytogenetics: 12p13.32
• Protein Families: Druggable Genome
• Summary: Arginine methylation is a widespread posttranslational modification mediated by arginine methyltransferases, such as PRMT8. Arginine methylation is involved in a number of cellular processes, including DNA repair, RNA transcription, signal transduction, protein compartmentalization, and possibly protein translation (Lee et al., 2005 [PubMed 16051612]).[supplied by OMIM, Mar 2008]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).