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NR2F2 (NM_021005) Human Untagged Clone

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SKU
SC108069
NR2F2 (untagged)-Human nuclear receptor subfamily 2, group F, member 2 (NR2F2), transcript variant 1
  $457.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol NR2F2
Synonyms ARP-1; ARP1; CHTD4; COUPTF2; COUPTFB; COUPTFII; NF-E3; SRXX5; SVP40; TFCOUP2
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_021005, the custom clone sequence may differ by one or more nucleotides


ATGGCAATGGTAGTCAGCACGTGGCGCGACCCCCAGGACGAGGTGCCCGGCTCACAGGGCAGCCAGGCCT
CGCAGGCGCCGCCCGTGCCCGGCCCGCCGCCCGGCGCCCCGCACACGCCACAGACGCCCGGCCAAGGGGG
CCCAGCCAGCACGCCAGCCCAGACGGCGGCCGGTGGCCAGGGCGGCCCTGGCGGCCCGGGTAGCGACAAG
CAGCAGCAGCAGCAACACATCGAGTGCGTGGTGTGCGGAGACAAGTCGAGCGGCAAGCACTACGGCCAGT
TCACGTGCGAGGGCTGCAAGAGCTTCTTCAAGCGCAGCGTGCGGAGGAACCTGAGCTACACGTGCCGCGC
CAACCGGAACTGTCCCATCGACCAGCACCATCGCAACCAGTGCCAGTACTGCCGCCTCAAAAAGTGCCTC
AAAGTGGGCATGAGACGGGAAGCGGTGCAGAGGGGCAGGATGCCGCCGACCCAGCCGACCCACGGGCAGT
TCGCGCTGACCAACGGGGATCCCCTCAACTGCCACTCGTACCTGTCCGGATATATTTCCCTGCTGTTGCG
CGCGGAGCCCTATCCCACGTCGCGCTTCGGCAGCCAATGCATGCAGCCCAACAACATCATGGGTATCGAG
AACATTTGCGAACTGGCCGCGAGGATGCTCTTCAGCGCCGTCGAGTGGGCCCGGAACATCCCCTTCTTCC
CCGACCTGCAGATCACGGACCAGGTGGCCCTGCTTCGCCTCACCTGGAGCGAGCTGTTTGTGTTGAATGC
GGCGCAGTGCTCCATGCCCCTCCACGTCGCCCCGCTCCTGGCCGCCGCCGGCCTGCATGCTTCGCCCATG
TCCGCCGACCGGGTGGTCGCCTTTATGGACCACATACGGATCTTCCAAGAGCAAGTGGAGAAGCTCAAGG
CGCTGCACGTTGACTCAGCCGAGTACAGCTGCCTCAAGGCCATAGTCCTGTTCACCTCAGATGCCTGTGG
TCTCTCTGATGTAGCCCATGTGGAAAGCTTGCAGGAAAAGTCTCAGTGTGCTTTGGAAGAATACGTTAGG
AGCCAGTACCCCAACCAGCCGACGAGATTCGGAAAGCTTTTGCTTCGCCTCCCTTCCCTCCGCACCGTCT
CCTCCTCAGTCATAGAGCAATTGTTTTTCGTCCGTTTGGTAGGTAAAACCCCCATCGAAACCCTCATCCG
GGATATGTTACTGTCCGGCAGCAGTTTTAACTGGCCGTATATGGCAATTCAATAA

5' Read Nucleotide Sequence
>OriGene 5' read for NM_021005 unedited
TAAAATTTGTATACGACTCATATAGGCGGCCGCGACATTCGCACGAGGCTTCTGTCTTGA
AGCCAGACAATCGACTTCAGCTCTCCCTCCCCTCCCTCTTTCTCCACGTTCTGCTCCCAC
TCGCTCTCCTGTCCCCTTCCCCTCCCCTCCCGGCGGAAAGCCCCCCGAAACCAACAAAGC
TGAGCCGAGAGAAACAAACAAAACAAACACACCGGGCCAGACAAGCCATCGACAAAACTT
TGCAAAAGCAAAAACAAAAAAGGAAAAACTAACCAACCTCAACCAACCAGCCCCCGAGCC
ACCCGGGGCGCCCTCCCGCGCCCTCTTGCACCCTCGCACACACAAAAGGCGGCGCGCCGG
AGCCCGAGACCCGGGGAGCCGCCGCCGCCCCGCCGCCGCCCGCAGCCAGGGGAGCAGGAA
GTCCGGACGCAGCCCCCATAGATATGGCAATGGTAGTCAGCACGTGGCGCGACCCCCAGG
ACGAGGTGCCCGGCTCACAGGGCAGCCAGGCCTCGCAGGCGCCGCCCGTGCCCGGCCCGC
CGCCCGGCGCCCCGCACACGCCACAGACGCCCGGCCAAGGGGGCCCAGCCAGCACGCCAG
CCCAGACGGCGGCCGGTGGCCAGGGCGGCCCTGGCGGCCCGGGTAGCGACAAGCAGCAGC
AGCAGCAACACATCGAGTGCGTGGTGTGCGGAGACAAGTCGAGCGGCAAGCACTACGGCC
AGTTCACGTGCGAAGGCTGNCAGAGCTTCTTCAAGCGCAGCGTGCGGAGGAACCTGAGCT
ACACGTGCCGCGCCAACCGGAACTGTCCCATCGACCAGCACCATCGCAACCAGTGCCAGT
ACTGCCGCCTCANAAGTGCCTCAAAGTGGGCATGAAACGGGAAAGCGTGCANNAGGGCAG
GATGCCGNCGACCCANCCGACCACGGGCAGTTNGCGCTGACAACGGGGATCCCTCACT
3' Read Nucleotide Sequence
>OriGene 3' read for NM_021005 unedited
GGACGCGGCCGCAATCTANATCGAGTTTTTTTTTTTTTTTTTTGAAATTACAGTCCATTA
AATTCTTCCCCTCTCTTCTTTTAGCAAACTTGTAACATCCTTTTATACCCTTTCTTAACA
GAAGGAAATTAAGCAAACAAACCAGTCTTTTGCCTTTTCTTTCTCTGTTTCACTCCCCCT
TCTTATTTTATTTATTTATTGAATTGCCATATACGGCCAGTTAAAACTGCTGCCGGACAG
TAACATATCCCGGATGAGGGTTTCGATGGGGGTTTTACCTACCAAACGGACGAAAAACAA
TTGCTCTATGACTGAGGAGGAGACGGTGCGGAGGGAAGGGAGGCGAATCAAAAGCTTTCC
GAATCTCGCCGGCTGGTTGGGGTACTGGCTCCTAACGTATTCTTCCAAAGCACACTGAGA
CTTNTCCTGCAAGCTTTCCACATGGGCTACATCAGAGAGACCACAGGCATCTGAGGTGAA
CAGGACTATGGCCTTGAGGCAGCTGTACTCGGCTGAGCAACGTGCAGCGCCTTGAGCTTC
TTCACTTGCTCTTGGAAGACCGTATGTGGTCCATAAAGGCGACCACCCGGCCGGCGGACA
TGGGCGAAGCATGCAGGCCGGCGGCGGCCATAACCGGTGCGACTTGGAGGGGCATGGACC
ACTGCGCCGAATTAACACAAACAGCTCGCTTCAGGTGAGGCGAAGCAGCGCACCTTGTCC
CTGATCTGCAAGTCCGGGAAGAATGCGATGTCCCGGCCCCCTCGACAGCGCTGAAAGCTT
CCTCCGGGCCAGTTCCAAATGTTCCGAACCCAAGATGTTGCCGCGCTGATGCCTTGCCTG
CCCAACCGGACTGGGAAATGCCTCCGCCCCACAAGCCGGGAAATTATTCCGCACGTTCCC
AGGGGAATCTGAGGGGATCCCCTTGGCACACCCAACTGCCCGGGTCCGTTGTGGCCGGGG
AATCTCGACCTTTGGCCGCTTATCGTCTATGCACATCT
Restriction Sites NotI-NotI |
ACCN NM_021005
Insert Size 2000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_021005.2, NP_066285.1
RefSeq Size 3049 bp
RefSeq ORF 1245 bp
Locus ID 7026
UniProt ID P24468
Cytogenetics 15q26.2
Domains HOLI, zf-C4
Protein Families Druggable Genome, Nuclear Hormone Receptor, Transcription Factors
Summary This gene encodes a member of the steroid thyroid hormone superfamily of nuclear receptors. The encoded protein is a ligand inducible transcription factor that is involved in the regulation of many different genes. Alternate splicing results in multiple transcript variants. provided by RefSeq, Mar 2010
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_021005" in other vectors (6)
SKU Description Size Price
RC206753 NR2F2 (Myc-DDK-tagged)-Human nuclear receptor subfamily 2, group F, member 2 (NR2F2), transcript variant 1 10 ug
$457.00
RC206753L1 Lenti-ORF clone of NR2F2 (Myc-DDK-tagged)-Human nuclear receptor subfamily 2, group F, member 2 (NR2F2), transcript variant 1 10 ug
$757.00
RC206753L2 Lenti-ORF clone of NR2F2 (mGFP-tagged)-Human nuclear receptor subfamily 2, group F, member 2 (NR2F2), transcript variant 1 10 ug
$757.00
RC206753L3 Lenti-ORF clone of NR2F2 (Myc-DDK-tagged)-Human nuclear receptor subfamily 2, group F, member 2 (NR2F2), transcript variant 1 10 ug
$757.00
RC206753L4 Lenti-ORF clone of NR2F2 (mGFP-tagged)-Human nuclear receptor subfamily 2, group F, member 2 (NR2F2), transcript variant 1 10 ug
$757.00
RG206753 NR2F2 (tGFP-tagged) - Human nuclear receptor subfamily 2, group F, member 2 (NR2F2), transcript variant 1 10 ug
$489.00 $657.00

file-alt Citations

Evidence forNR2F2/COUP-TFII involvement in human testis development
Wankanit, Zidoune, Bignon-Topalovic et al
bioRxiv (2024)
Cited by 1
Snippet: pCMV6-XL5 NR2F2 (pCMV6-NR2F2, #SC108069, Origene) was used for evaluating COUP300 TFII functions in this study. NR2F2 expression vector containing the NM_021005.4:c.737GA 301 (p.Arg246His) variant was generated by site-directed mutagenesis (QuikChange II, Stratagene), 302 using the forward primer: 5’-GACCAGGTGGCCCTGCTTCACCTCACCTGGAGCGAGC-3’ 303 and the reverse primer 5’-GCTCGCTCCAGGTGAGGTGAAGCAGGGCCACCTGGTC-3’
Forkhead Box Transcription Factors of the FOXA Class Are Required for Basal Transcription of Angiotensin-Converting Enzyme 2
Pedersen, Chodavarapu, Lazartigues
J Endocr Soc (2017) 1 (4), 370-384 DOI: 10.1210/js.2016-1071
AI Summary: OriGene contributed to this study by providing the COUP-TFII expression plasmid (catalog no. sc108069), which was used to investigate transcriptional regulation of ACE2.
Snippet: A COUP-TFII expression plasmid was purchased from Origene (catalog no. sc108069). A FOXO1 expression plasmid, pcDNA3-FKHR, was a kind gift from Dr. Andrew Hollenbach (Louisiana State University Health Sciences Center, New Orleans, LS)
FigureFigureFigure
NR2F2 regulates chondrogenesis of human mesenchymal stem cells in bioprinted cartilage
Gao, Zhang, Hubbell et al
Biotechnol. Bioeng. (2016)
Cited by 39
AI Summary: OriGene’s pCMV6-XL5-Nr2f2 plasmid was used as the source of human NR2F2 cDNA to generate lentivirus for overexpressing NR2F2 in mesenchymal stem cells, enabling the study of NR2F2’s role in chondrogenesis.
Snippet: Lentivirus to Over-Express NR2F2 in MSCs. The human NR2F2 cDNA was amplified from pCMV6-XL5-Nr2f2 (OriGene, Rockville, MD) and sub-cloned into pCDH-EF1-T2A-GFP lentiviral vector (System Biosciences, Palo Alto, CA) between EcoRI and NotI digestion ... Cite SaveSaving
Venous Endothelial Marker COUP-TFII Regulates the Distinct Pathologic Potentials of Adult Arteries and Veins
Cui, Lu, Lee et al
Sci Rep (2015) 5, 16193 DOI: 10.1038/srep16193
Cited by 61
AI Summary: OriGene’s pCMV6-XL5-Nr2f2 plasmid was used as the source of human COUP-TFII cDNA for lentiviral overexpression experiments, enabling the study of COUP-TFII’s role in regulating vascular endothelial cell phenotypes in this paper.
Snippet: Lentivirus to Over-express COUP-TFII. The human Nr2f2 (COUP-TFII) cDNA was amplified from pCMV6-XL5-Nr2f2 (OriGene) by PCR, then sub-cloned into pCDH-EF1-T2A-GFP lentiviral vector (System Biosciences) between EcoRI and NotI digestion sites. ... Cite SaveSaving
FigureFigureFigure
Showing 1-5 of 5 papers.
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