PDLIM1 (NM_020992) Human Untagged Clone

SKU SC108058

PDLIM1 (untagged)-Human PDZ and LIM domain 1 (PDLIM1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: PDLIM1
• Synonyms: CLIM1; CLP-36; CLP36; hCLIM1; HEL-S-112
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108058 sequence for NM_020992 edited (data generated by NextGen Sequencing); ATGACCACCCAGCAGATAGACCTCCAGGGCCCGGGGCCGTGGGGCTTCCGCCTCGTGGGC; GGCAAGGACTTCGAGCAGCCTCTCGCCATTTCCCGGGTCACTCCTGGAAGCAAGGCGGCT; CTAGCTAATTTATGTATTGGAGATGTAATCACAGCCATTGATGGGGAAAATACTAGCAAT; ATGACACACTTGGAAGCTCAGAACAGAATCAAAGGCTGCACAGACAACTTGACTCTCACT; GTAGCCAGATCTGAACATAAAGTCTGGTCTCCTCTGGTGACGGAGGAAGGGAAGCGTCAT; CCATACAAGATGAATTTAGCCTCTGAACCCCAGGAGGTCCTGCACATAGGAAGCGCCCAC; AACCGAAGTGCCATGCCCTTTACCGCCTCGCCTGCCTCCAGCACTACTGCCAGGGTCATC; ACAAACCAGTACAACAACCCAGCTGGCCTCTACTCTTCTGAAAATATCTCCAACTTCAAC; AATGCCCTGGAGTCAAAGACTGCTGCCAGCGGGGTGGAGGCGAACAGCAGACCCTTAGAC; CATGCTCAGCCTCCAAGCAGCCTTGTCATCGACAAAGAATCTGAAGTTTACAAGATGCTT; CAGGAGAAACAGGAGTTGAATGAGCCCCCGAAACAGTCCACGTCTTTCTTGGTTTTGCAG; GAAATCCTGGAGTCTGAAGAAAAAGGGGATCCCAACAAGCCCTCAGGATTCAGAAGTGTT; AAAGCTCCTGTCACTAAAGTGGCTGCGTCGATTGGAAATGCTCAGAAGTTGCCTATGTGT; GACAAATGTGGCACTGGGATTGTTGGTGTGTTTGTGAAGCTGCGGGACCGTCACCGCCAC; CCTGAGTGTTATGTGTGCACTGACTGTGGCACCAACCTGAAACAGAAGGGCCATTTCTTT; GTGGAGGATCAAATCTACTGTGAGAAGCATGCCCGGGAGCGAGTCACACCACCTGAGGGT; TATGAAGTGGTCACTGTGTTCCCCAAGTGA; ; Clone variation with respect to NM_020992.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_020992 unedited; ACATTTTGTAAACGACTCACTATAGGCGGCCGCGAATTCGCACGAGCCCGGGCCCGCGCC; GCGCCGCTCTTTCTCCGACAGCCGCCGGGGGTGCCCTGCAAGCTGTTCCGCGCGTCCTGC; CCGTCTGTCCCCGCGGGTCGTCGCCCGCCACAGCCGCGCCATGACCACCCAGCAGATAGA; CCTCCAGGGCCCGGGGCCGTGGGGCTTCCGCCTCGTGGGCGGCAAGGACTTCGAGCAGCC; TCTCGCCATTTCCCGGGTCACTCCTGGAAGCAAGGCGGCTCTAGCTAATTTATGTATTGG; AGATGTAATCACAGCCATTGATGGGGAAAATACTAGCAATATGACACACTTGGAAGCTCA; GAACAGAATCAAAGGCTGCACAGACAACTTGACTCTCACTGTAGCCAGATCTGAACATAA; AGTCTGGTCTCCTCTGGTGACGGAGGAAGGGAAGCGTCATCCATACAAGATGAATTTAGC; CTCTGAACCCCAGGAGGTCCTGCACATAGGAAGCGCCCACAACCGAAGTGCCATGCCCTT; TACCGCCTCGCCTGCCTCCAGCACTACTGCCAGGGTCATCACAAACCAGTACAACAACCC; AGCTGGCCTCTACTCTTCTGAAAATATCTCCAACTTCAACAATGCCCTGGAGTCAAAGAC; TGCTNGCCAGCGNGTGGAGGCGAACAGCAGACCCTTAGACCATGCTCAGCCTCCAAGCAG; CCTTGTCATCGACAAAGAATCTGAAGTTTACAAGATGCTTCANGAGAAACAGGAGTTGAA; TGAGCCCCCGAACAGTCCCACGTCTTCTTGGTTTTGCAGGGAATCCTGGAGTCTGAAGAA; AAGGGGATCCCNACAGCCCTCAGGATTCAGAAGTGTTNAAGCTNCTGTCACTAAATGGCT; GCGTCGATTGGAATGCTCANAAGTTGCCTTGTGTGACAATGTGCACTGGGATGTTTGGTG; GTTTGGAAAC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_020992 unedited; NGGTCTTTTATGNACGCGGCCGCATTCTANGATCGANCTAGTTCTCTCCTAGTCTCGAGT; TTTTTTTTTTTTTTTTTTGCTTTGTAGTCTATACATTTATTGAGTAAAAACAAAATCAGT; GTCAGACACGTTATATTTGATTGGGTTCAAATTTGGCTGATGTCCAAATGCAGCAGAGAA; GAACGGTGGGGCGAAGGGACACAGTGTTGCTGACAAGGTGACACTGAACAAAACAGTTTT; CCTTTAATTGTAAAAGCGGGCATCGCACAGCTGGTGTGAGTCAATTAACCAAGGCAGGGA; GGGGACTCAATGTTTTACAAGCAGAGGGAAAACCAAAGTAAGCAGAGAACTTTCAAGAGA; GGAGAGGGCCAGAACACTGAGAGAAAAAGCTGCAGCAGAGGCCTGCTGGAGAACAGTGGT; CAGATCTGCTGGCTCACTTGGGGAACACAGTGACCACTTCATAACCCTCAGGTGGTGTGA; CTCGCTCCCGGGCATGCTTCTCACAGTAGATTTGATCCTCCACAAAGAAATGGCCCTTCT; GTTTCAGGTTGGTGCCACAGTCAGTGCACACATAACACTCAGGGTGGCGGTGACGGTCCC; GCAGCTTCACAAACACACCAACAATCCCAGTGCCACATTTGTCACACATAGGCAACTTCT; GAGCATTTCCAATCGACGCAGCCACTTTAGTGACAGGAGCTTTAACACTTCTGAATCCTN; GAGGCTTGTTGGGATCCCCTTTTTCTTCAGACTCCAAGATTTCCTGCAAAACCAAGAAAG; ACGTGGACTGTTTTCGGGGCTCATTCAACTCCTGTTTCTCCTGAAGCATCTTGTAAACTT; CAGATTCTTTGTCGATGACCAGGCTGCTTTGGAGCTGAGCATGGTCTAN;
• Restriction Sites: NotI-NotI
• ACCN: NM_020992
• Insert Size: 1650 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_020992.2, NP_066272.1
• RefSeq Size: 1462 bp
• RefSeq ORF: 990 bp
• Locus ID: 9124
• UniProt ID: O00151
• Cytogenetics: 10q23.33
• Domains: LIM, PDZ, ZM
• Summary: This gene encodes a member of the enigma protein family. The protein contains two protein interacting domains, a PDZ domain at the amino terminal end and one to three LIM domains at the carboxyl terminal. It is a cytoplasmic protein associated with the cytoskeleton. The protein may function as an adapter to bring other LIM-interacting proteins to the cytoskeleton. Pseudogenes associated with this gene are located on chromosomes 3, 14 and 17. [provided by RefSeq, Oct 2012]