BAD (NM_004322) Human Untagged Clone
$300.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | BAD |
| Synonyms | BBC2; BCL2L8 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites | NotI-NotI | |
| ACCN | NM_004322 |
| Insert Size | 1110 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_004322.2, NP_004313.1 |
| RefSeq Size | 1127 bp |
| RefSeq ORF | 507 bp |
| Locus ID | 572 |
| UniProt ID | Q92934 |
| Cytogenetics | 11q13.1 |
| Protein Families | Druggable Genome |
| Protein Pathways | Acute myeloid leukemia, Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Focal adhesion, Insulin signaling pathway, Melanoma, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer, VEGF signaling pathway |
| Summary | The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL (B-cell lymphoma-extra large) and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. provided by RefSeq, Dec 2019 Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein. |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_004322" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC200634 | BAD (Myc-DDK-tagged)-Human BCL2-associated agonist of cell death (BAD), transcript variant 1 | 10 ug |
$300.00
|
|
| RC200634L3 | Lenti ORF clone of Human BCL2-associated agonist of cell death (BAD), transcript variant 1, Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC200634L4 | Lenti ORF clone of Human BCL2-associated agonist of cell death (BAD), transcript variant 1, mGFP tagged | 10 ug |
$600.00
|
|
| RG200634 | BAD (tGFP-tagged) - Human BCL2-associated agonist of cell death (BAD), transcript variant 1 | 10 ug |
$489.00
$500.00
|
|
Citations
Identification of CD44 as a surface biomarker for drug resistance by surface proteome signature technology
Cain, Hauptschein, Stewart et al
Mol. Cancer Res. (2011) 9 (5), 637-47 DOI: 10.1158/1541-7786.MCR-09-0237
Cain, Hauptschein, Stewart et al
Mol. Cancer Res. (2011) 9 (5), 637-47 DOI: 10.1158/1541-7786.MCR-09-0237
Cited by 5
AI Summary: In this study, OriGene's Bcl-xL expression vector was used to transfect NCI/ADR-RES cells, enabling the researchers to investigate the role of Bcl-xL in drug resistance mechanisms alongside the identification of CD44 as a surface biomarker.
Snippet: siRNA–treated NCI/ADR-RES cells were plated in a 6-well plate and transfected with a Bcl-xL expression vector (Origene) at a 1 ... The expression of the poliovirus receptor CD155, the antigen for scFv 5d11, was knocked down in HT1080 cells and confirmed via Western blot and
BAG-1 overexpression attenuates luminal apoptosis in MCF-10A mammary epithelial cells through enhanced RAF-1 activation
Anderson, Sutherland, Butt
Oncogene (2010) 29 (4), 527-38 DOI: 10.1038/onc.2009.362
Anderson, Sutherland, Butt
Oncogene (2010) 29 (4), 527-38 DOI: 10.1038/onc.2009.362
Cited by 24
AI Summary: In this study, OriGene’s BAG-1 overexpression plasmid (RC204307) was used to generate MCF-10A cells with elevated BAG-1 levels, enabling the authors to investigate how BAG-1 influences apoptosis and RAF-1 activation during mammary epithelial morphogenesis.
BAG-1 predicts patient outcome and tamoxifen responsiveness in ER-positive invasive ductal carcinoma of the breast
Millar, Anderson, McNeil et al
Br J Cancer (2009) 100 (1), 123-33 DOI: 10.1038/sj.bjc.6604809
Millar, Anderson, McNeil et al
Br J Cancer (2009) 100 (1), 123-33 DOI: 10.1038/sj.bjc.6604809
Cited by 41
AI Summary: In this study, OriGene’s BAG-1 cDNA (OriGene, SC119430) was used to generate BAG-1-overexpressing MCF-7 breast cancer cells, enabling the authors to investigate the impact of BAG-1 on tamoxifen sensitivity and breast cancer patient outcomes.
Showing 1-3 of 3 papers.
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