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DFFB (NM_004402) Human Untagged Clone

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SKU
SC107911
DFFB (untagged)-Human DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase) (DFFB)
  $457.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol DFFB
Synonyms CAD; CPAN; DFF-40; DFF2; DFF40
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC107911 sequence for NM_004402 edited (data generated by NextGen Sequencing)
ATGCTCCAGAAGCCCAAGAGCGTGAAGCTGCGGGCCCTGCGCAGCCCGAGGAAGTTCGGC
GTGGCTGGCCGGAGCTGCCAGGAGGTGCTGCGCAAGGGCTGTCTCCGCTTCCAGCTCCCT
GAGCGCGGTTCCCGGCTGTGCCTGTACGAGGATGGCACGGAGCTGACGGAAGATTACTTC
CCCAGTGTTCCCGACAACGCCGAGCTGGTGCTGCTCACCTTGGGCCAGGCCTGGCAGGGC
TATGTGAGCGACATCAGGCGCTTCCTCAGTGCATTTCACGAGCCACAGGTGGGGCTCATC
CAGGCCGCCCAGCAGCTGCTGTGTGATGAGCAGGCCCCACAGAGGCAGAGGCTGCTGGCT
GACCTCCTGCACAACGTCAGCCAGAACATCGCGGCCGAGACCCGGGCTGAGGACCCGCCG
TGGTTTGAAGGCTTGGAGTCCCGATTTCAGAGCAAGTCTGGCTATCTGAGATACAGCTGT
GAGAGCCGGATCCGGAGTTACCTGAGGGAGGTGAGCTCCTACCCCTCCACGGTGGGTGCG
GAGGCTCAGGAGGAATTCCTGCGGGTCCTCGGCTCCATGTGCCAGAAGCTCCGGTCCATG
CAGTACAATGGCAGCTACTTCGACAGAGGAGCCAAGGGCGGCAGCCGCCTCTGCACACCG
GAAGGCTGGTTCTCCTGCCAGGGTCCCTTTGACATGGACAGCTGCTTATCAAGACACTCC
ATCAACCCCTACAGTAACAGGGAGAGCAGGATCCTCTTCAGCACCTGGAACCTGGATCGC
ATAATAGAAAAGAAACGCACCATCATTCCTACACTGGTGGAAGCAATTAAGGAACAAGAT
GGAAGAGAAGTGGACTGGGAGTATTTTTATGGCCTGCTTTTTACCTCAGAGAACCTAAAA
CTAGTGCACATTGTCTGCCATAAGAAAACCACCCACAAGCTCAACTGTGACCCAAGCAGA
ATCTACAAACCCCAGACAAGGTTGAAGCGGAAGCAGCCTGTGCGGAAACGCCAGTGA

Clone variation with respect to NM_004402.2
587 g=>a;779 a=>g
5' Read Nucleotide Sequence
>OriGene 5' read for NM_004402 unedited
AAAAAAAGGGGANNNANAANNAAAAACACCCCCCCGGTTCACAATTGTATACGACTCATA
TAGGCGGCCGCGNAATTCGCACGAGGCCAGCTTGCAGAGCTCACCAGGTGCAGACCCCTG
CGGCCAGGGCGAGGACGGATCTGAGCAGCTGGGCAGCAGGTGCCACCGCCTGTGGGACCC
AGAGGGCTTGAGGACATCTGCAATGCTCCAGAAGCCCAAGAGCGTGAAGCTGCGGGCCCT
GCGCAGCCCGAGGAAGTTCGGCGTGGCTGGCCGGAGCTGCCAGGAGGTGCTGCGCAAGGG
CTGTCTCCGCTTCCAGCTCCCTGAGCGCGGTTCCCGGCTGTGCCTGTACGAGGATGGCAC
GGAGCTGACGGAAGATTACTTCCCCAGTGTTCCCGACAACGCCGAGCTGGTGCTGCTCAC
CTTGGGCCAGGCCTGGCAGGGCTATGTGAGCGACATCAGGCGCTTCCTCAGTGCATTTCA
CGAGCCACAGGTGGGGCTCATCCAGGCCGCCCAGCAGCTGCTGTGTGATGAGCAGGCCCC
ACAGAGGCAGAGGCTGCTGGCTGACCTCCTGCACAACGTCAGCCAGAACATCGCGGCCGA
GACCCGGGCTGAGGACCCGCCGTGGTTTGAAGGCTTGGAGTCCCGATTTCAGAGCAAGTC
TGGCTATCTGAGATACAGCTGTGAGAGCCCNATCCGGAGTTACCTGNAGGAGGTGAGCTC
CTACCCCTCCCACGTGGGTGCGGAGGCTCCAGAGGAATTCCTGCCGCTCCTCCGCTCCAT
GGGCCANAAGCTTCCGTCCATGCAGTACAATGGCAGCTACTTCCACAGAGGAGCCAAGGC
CGGCAGCCGCCTTTGCACACCGGAAGGGCTGTTCTCCCTGCCGGGTCCCTTTGACAGGAA
CAGCTGCTTAAN
3' Read Nucleotide Sequence
>OriGene 3' read for NM_004402 unedited
NNTTTCTGTGNACCGCGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTTTATTTT
TGAAAAATCTTTATTGCTTTTCCTGATTGTACAACAAATGTAAAAATTCAAATATTACAA
AATGACTTAATTTAAAAAGTACATTTTAACATGTTATAAAATAAATGCCCATTTCAAAAA
ATTCAAGTATCATAAAATACATGACGTAGAAAATGAGTCCTCTGTAATCTAGCCCAACCC
CCGTATCCCCCAGGATTACTGCTGTTCAGATCCGCGTGTTTCCTTCCAGACCGTCTCGTG
GTGNCNNNNNACCCNCCCGGNCCNCCCAACCCCCCCAACCAACGGGGCCTTGGACCATGA
AGCCTTCCATATTGAATCCCGNAGNATGAAATAGTTAACCGCTGAACCCAACGGAAGCCA
AGGGTTTCAANCTGCCCGGATGCCCCCTGGAAACAAGCTTCCCAGTTTTCCTTAACACCA
GAAAACCCCTTTTTCCCTTCAATAAAAAAACCCTTGGAAAAACCTGGGGGAACACCAAAT
TTTTTTGGCCCCTTTTGGTAAAATGGTATTCAAGGGTTGGAAATCCCTTGAAAAATAAAA
TGGGATACCCCTTAACCCATTTTTTTTGGGCCCCTTAAAAAAAAAAAACAAGAGTTTTCC
CCCGGGGGTTCAAAACGGGGAAAAAAACTTTGGTTTTCAAAAAAAAAACAATTTGGGGGG
GGGGGGGTTTTCCCCCTTTTTTTTCTCTGTGGCCCCTTTTTTTAATAAAACAAATTTTTC
AACCCGGGGGGGTCACGAGGGGGCCCCCACGGGGGGGGTTTTAAAAGCCCCCCCGGTGAT
AAACCCCCCCCCACCACCCTC
Restriction Sites NotI-NotI |
ACCN NM_004402
Insert Size 3340 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004402.2, NP_004393.1
RefSeq Size 3034 bp
RefSeq ORF 1017 bp
Locus ID 1677
UniProt ID O76075
Cytogenetics 1p36.32
Domains CAD
Protein Families Druggable Genome
Protein Pathways Apoptosis
Summary Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of some of these variants has not been determined. provided by RefSeq, Sep 2013
Transcript Variant: This variant (2) contains two consecutive alternate splice junctions compared to variant 1. The resulting isoform (2) has the same N- and C-termini but lacks an internal segment compared to isoform 1.
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"NM_004402" in other vectors (6)
SKU Description Size Price
RC208266 DFFB (Myc-DDK-tagged)-Human DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase) (DFFB) 10 ug
$289.00 $457.00
RC208266L1 Lenti ORF clone of Human DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase) (DFFB), Myc-DDK-tagged 10 ug
$757.00
RC208266L2 Lenti ORF clone of Human DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase) (DFFB), mGFP tagged 10 ug
$757.00
RC208266L3 Lenti ORF clone of Human DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase) (DFFB), Myc-DDK-tagged 10 ug
$757.00
RC208266L4 Lenti ORF clone of Human DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase) (DFFB), mGFP tagged 10 ug
$757.00
RG208266 DFFB (tGFP-tagged) - Human DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase) (DFFB) 10 ug
$489.00 $657.00

file-alt Citations

Caspase-dependent cell death-associated release of nucleosome and damage-associated molecular patterns
Yoon, Park, Han et al
Cell Death Dis (2014) 5, e1494 DOI: 10.1038/cddis.2014.450
Cited by 27
AI Summary: OriGene products contributed to this study by providing cDNAs for caspase 3, CAD, EndoG, calpain 1, and calpain 2, which were used to investigate the molecular mechanisms underlying caspase-dependent regulated necrosis and the release of nucleosomes and DAMPs.
Snippet: cDNAs of caspase 3, CAD, EndoG, calpain 1 and calpain 2 were purchased from OriGene (Rockville, MD, USA), calpastatin cDNA was from Shi-Yong Sun (Emory University School of Medicine), being subcloned into pCDH-EF2-MCS-T2A-Puro, a lentiviral vector for ... Cite SaveSaving
Showing 1-1 of 1 papers.
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