MARK4 (NM_031417) Human Untagged Clone

SKU SC107258

MARK4 (untagged)-Human MAP/microtubule affinity-regulating kinase 4 (MARK4), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: MARK4
• Synonyms: MARK4L; MARK4S; MARKL1; MARKL1L; PAR-1D
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_031417, the custom clone sequence may differ by one or more nucleotides; ; ; ATGTCTTCGCGGACGGTGCTGGCCCCGGGCAACGATCGGAACTCGGACACGCATGGCACCTTGGGCAGTG; GCCGCTCCTCGGACAAAGGCCCGTCCTGGTCCAGCCGCTCACTGGGTGCCCGTTGCCGGAACTCCATCGC; CTCCTGTCCCGAGGAGCAGCCCCACGTGGGCAACTACCGCCTGCTGAGGACCATTGGGAAGGGCAACTTT; GCCAAAGTCAAGCTGGCTCGGCACATCCTCACTGGTCGGGAGGTTGCCATCAAGATTATCGACAAAACCC; AGCTGAATCCCAGCAGCCTGCAGAAGCTGTTCCGAGAAGTCCGCATCATGAAGGGCCTAAACCACCCCAA; CATCGTGAAGCTCTTTGAGGTGATTGAGACTGAGAAGACGCTGTACCTGGTGATGGAGTACGCAAGTGCT; GGAGAAGTGTTTGACTACCTCGTGTCGCATGGCCGCATGAAGGAGAAGGAAGCTCGAGCCAAGTTCCGAC; AGATTGTTTCGGCTGTGCACTATTGTCACCAGAAAAATATTGTACACAGGGACCTGAAGGCTGAGAACCT; CTTGCTGGATGCCGAGGCCAACATCAAGATTGCTGACTTTGGCTTCAGCAACGAGTTCACGCTGGGATCG; AAGCTGGACACGTTCTGCGGGAGCCCCCCATATGCCGCCCCGGAGCTGTTTCAGGGCAAGAAGTACGACG; GGCCGGAGGTGGACATCTGGAGCCTGGGAGTCATCCTGTACACCCTCGTCAGCGGCTCCCTGCCCTTCGA; CGGGCACAACCTCAAGGAGCTGCGGGAGCGAGTACTCAGAGGGAAGTACCGGGTCCCTTTCTACATGTCA; ACAGACTGTGAGAGCATCCTGCGGAGATTTTTGGTGCTGAACCCAGCTAAACGCTGTACTCTCGAGCAAA; TCATGAAAGACAAATGGATCAACATCGGCTATGAGGGTGAGGAGTTGAAGCCATACACAGAGCCCGAGGA; GGACTTCGGGGACACCAAGAGAATTGAGGTGATGGTGGGTATGGGCTACACACGGGAAGAAATCAAAGAG; TCCTTGACCAGCCAGAAGTACAACGAAGTGACCGCCACCTACCTCCTGCTGGGCAGGAAGACTGAGGAGG; GTGGGGACCGGGGCGCCCCAGGGCTGGCCCTGGCACGGGTGCGGGCGCCCAGCGACACCACCAACGGAAC; AAGTTCCAGCAAAGGCACCAGCCACAGCAAAGGGCAGCGGAGTTCCTCTTCCACCTACCACCGCCAGCGC; AGGCATAGCGATTTCTGTGGCCCATCCCCTGCACCCCTGCACCCCAAACGCAGCCCGACGAGCACGGGGG; AGGCGGAGCTGAAGGAGGAGCGGCTGCCAGGCCGGAAGGCGAGCTGCAGCACCGCGGGGAGTGGGAGTCG; AGGGCTGCCCCCCTCCAGCCCCATGGTCAGCAGCGCCCACAACCCCAACAAGGCAGAGATCCCAGAGCGG; CGGAAGGACAGCACGAGCACCCCCAACAACCTCCCTCCTAGCATGATGACCCGCAGAAACACCTACGTTT; GCACAGAACGCCCGGGGGCTGAGCGCCCGTCACTGTTGCCAAATGGGAAAGAAAACAGCTCAGGCACCCC; ACGGGTGCCCCCTGCCTCCCCCTCCAGTCACAGCCTGGCACCCCCATCAGGGGAGCGGAGCCGCCTGGCA; CGCGGTTCCACCATCCGCAGCACCTTCCATGGTGGCCAGGTCCGGGACCGGCGGGCAGGGGGTGGGGGTG; GTGGGGGTGTGCAGAATGGGCCCCCTGCCTCTCCCACACTGGCCCATGAGGCTGCACCCCTGCCCGCCGG; GCGGCCCCGCCCCACCACCAACCTCTTCACCAAGCTGACCTCCAAACTGACCCGAAGGGTTACCCTCGAT; CCCTCTAAACGGCAGAACTCTAACCGCTGTGTTTCGGGCGCCTCTCTGCCCCAGGGATCCAAGATCAGGT; CGCAGACGAACCTGAGAGAATCGGGGGACCTGAGGTCACAAGTTGCCATCTACCTTGGGATCAAACGGAA; ACCGCCCCCCGGCTGCTCCGATTCCCCTGGAGTGTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_031417 unedited; CGGTTTTCGGGCAGTGNCTGGCCCCCGGTTCAACNTNCGGNAACTCGCCACGCATGGCAC; CTTGGGCAGTGGCCGCTCCTCGGNCAAAGGCCCGTCCTGGTCCAGCCGCTCACTGGGTGC; CCGTTGCCGGAACTCCATCGCCTCCTGTCCCGAGGAGCAGCCCCACGTGGGCAACTACCG; CCTGCTGAGGACCATTGGGAAGGGCAACTTTGCCAAAGTCAAGCTGGCTCGGCACATCCT; CACTGGTCGGGAGGTTGCCATCAAGATTATCGACAAAACCCAGCTGAATCCCAGCAGCCT; GCAGAAGCTGTTCCGAGAAGTCCGCATCATGAAGGGCCTAAACCACCCCAACATCGTGAA; GCTCTTTGAGGTGATTGAGACTGAGAAGACGCTGTACCTGGTGATGGAGTACGCAAGTGC; TGGAGAAGTGTTTGACTACCTCGTGTCGCATGGCCGCATGAAGGAGAAGGAAGCTCGAGC; CAAGTTCCGACAGATTGTTTCGGCTGTGCACTATTGTCACCAGAAAAATATTGTACACAG; GGACCTGAAGGCTGAGAACCTCTTGCTGGATGCCGAGGCCAACATCAAGATTGCTGACTT; TGGCTTCAGCAACGAGTTCACGCTGGGATCGAAGCTGGACACGTTCTGCGGGAGCCCCCC; ATATGCCGCCCCGGAGCTGTTTCAGGGCAAGAAGTACGACGGGCCGGGAGTGGACATCTG; GAGCCTGGGAGTCATCCTGTACACCCTCGTCAGCGGCTCCCTGCCCTTCGACGGGCACAA; CCTCAAAGAGCTGCGGGAGCGAGTACTCAGAGGGAAGTACGGGGTCCCTTTCTACATGTC; AACAGACTGTGAGAGCATCCTGCGGAGAATTTTGGTGCTGAACCCAGCTAAACGCTGTAC; TCTCGAGCAATCATGAAAGACAATGGAT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_031417 unedited; NNAAATTACTTGNACCGCGCCGCTTTCNANNGATCGGTTTTTTTTTTTTTTTTTTTTTTT; GGGGAGGGGGGGACTCTCTTAAATTATTGGGCAACAGGCTGCAGGTGAGGGGGCTGACAG; GAGGAGGGTCGGGGTGTAATAACTTAAAAACCGTAGGTGACAGCGGCAGCTCCTCAAGAG; ACCTTTGCAGAGGGGAGGGTGTGGGGTATTTACAAGGATTTGTACAAAGTGCCCCGCGCC; AGCCTGGGGCAGGAAGTGGGGGTTTGAGCGTGAGGGGACGGGAGGGGAAGGAATCTGCCT; CTCTGACCCCCACTCCCAGTCCCCCAGATTCTCCAGTCTTTAGGCTCAGCAGCTCTCCCT; GCCCCTGCCCAAGGCCTAATTTTGAGCTGTTGGGAGGAATGGAAGATTTCCTCAGTTTCC; CTCCCTGCCCCTGCCCTGTCCCCAAAGTCTCCTCAGGTGCCCTCTTGGTAGGGCGAGGGA; GGACAGGACTGGCTGGAGGCTCCAGGAAGGTGCCAGCTCCCCCCGCTGAGCCCCCTCATC; CTTCTTCTGTGCTGAGCGGCCCCAGAGAACAGCAGAGGGGACAATCTTTGCCCCCAAATA; TAATTCANGGAAACTGAGGTGATGATAAAGGGAGAATCCCCTCCCTGGCCCCTTCCCCTC; CTGTAGAAGTGAANGCGACAAGGGAGAAGGAAGAGTAAGGGCCCTGGGGACCGTGGTGGC; TCAGAGCTCGAGGTCGTTTGAAGATGCGGGTGACAAGGGTGCCGAAGGCCAGGGCGGTGC; CCGCCACACCGCGGAAGAGAACTCCCCGCAAGCCTGGCCCGGGCAGCTTGGCAAACCTCC; ACTCCAAATTGGGACAAGGGCTTGGGGCCCCGCCCGACCCCCGTGCAGGCAGGCCCAACA; GAAACGGTTTGGGCTGGCGGCCACCGGCC;
• Restriction Sites: Please inquire
• ACCN: NM_031417
• Insert Size: 5000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_031417.2, NP_113605.2
• RefSeq Size: 4917 bp
• RefSeq ORF: 2067 bp
• Locus ID: 57787
• UniProt ID: Q96L34
• Cytogenetics: 19q13.32
• Protein Families: Druggable Genome, Protein Kinase
• Summary: This gene encodes a member of the microtubule affinity-regulating kinase family. These protein kinases phosphorylate microtubule-associated proteins and regulate the transition between stable and dynamic microtubules. The encoded protein is associated with the centrosome throughout mitosis and may be involved in cell cycle control. Expression of this gene is a potential marker for cancer, and the encoded protein may also play a role in Alzheimer's disease. Pseudogenes of this gene are located on both the short and long arm of chromosome 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]; Transcript Variant: This variant (2) includes an alternate exon, which results in a frameshift, compared to variant 1. The resulting protein (isoform 2) is shorter and has a distinct C-terminus, compared to isoform 1.