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FRMD8 (NM_031904) Human Untagged Clone

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SKU
SC107202
FRMD8 (untagged)-Human FERM domain containing 8 (FRMD8)
  $457.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol FRMD8
Synonyms FKSG44; iTAP
Vector pCMV6-XL6
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC107202 sequence for NM_031904 edited (data generated by NextGen Sequencing)
ATGGACGGGACAGAAGGCAGTGCCGGGCAGCCCGGCCCCGCTGAGCGATCCCACCGAAGC
AGCGTGTCCTCCGTGGGAGCCCGAGCGGCTGACGTGCTGGTATACCTAGCGGATGACACG
GTGGTGCCCCTGGCTGTGGAGAACCTGCCCTCGCTCAGTGCCCATGAGCTGCACCGCGCT
GTCCGCGAGGTCCTGCAGCTTCCAGACATCGCCCTGGATGTCTTCGCGCTCTGGCTGGTC
TCCCCTCTGCTGGAGGTGCAGCTGAAACCCAAGCACCAGCCCTACAAGCTGGGACGCCAG
TGGCCGGAGCTGCTGCTGCGCTTCACCAGTGCCCCAGATGATGACGTGGCCATGGATGAG
CCTTTCCTGCAGTTCCGAAGGAACGTGTTCTTCCCAAAGCGGCGGGAGCTCCAGATCCAT
GACGAGGAGGTCCTGCGGCTGCTCTATGAGGAGGCCAAGGGCAACGTGCTGGCTGCACGG
TACCCGTGCGACGTGGAGGACTGCGAGGCTCTGGGCGCCCTGGTGTGCCGCGTGCAGCTT
GGGCCCTACCAGCCCGGCCGGCCGGCAGCCTGCGACCTGAGGGAGAAGCTGGACTCCTTC
CTCCCTGCCCACCTCTGTAAGCGGGGCCAGAGTCTCTTTGCTGCCCTCCGGGGCCGTGGG
GCCAGGGCCGGGCCGGGCGAGCAGGGCCTGCTGAACGCCTACCGCCAGGTGCAGGAGGTC
AGCAGCGACGGCGGGTGCGAGGCCGCCCTGGGCACCCACTACCGCGCCTATCTCCTCAAG
TGCCACGAGCTGCCGTTTTATGGGTGTGCCTTCTTCCACGGTGAGGTTGACAAGCCGGCC
CAAGGCTTTTTGCACCGGGGTGGGCGCAAGCCAGTTTCTGTGGCCATCAGTCTGGAAGGC
GTGCACGTCATCGATAGCAGAGAGAAGCATGTCCTGCTGGGCCTGCGCTTCCAGGAGCTG
TCGTGGGACCACACCTCCCCCGAGGAGGAGGAGCCCATCTTGTGGCTGGAGTTCGACGGG
GACAGCGAGGGCACACCTGTCAACAAGCTCCTCAAGATCTACTCCAAGCAGGCCGAACTG
ATGAGCAGTCTCATTGAGTACTGCATCGAACTGAGCCAGGTGGCGGAGCCCGCAGGCCCC
CAGGACAGTGCGACTGGCTCGCCCTCGGACCCCAGCTCCTCACTGGCTCCTGTTCAGCGC
CCCAAGCTGCGGAGGCAGGGCAGTGTGGTGTCCAGCCGGATCCAGCATCTCTCCACCATC
GACTACGTGGAGGACGGCAAGGGGATCAGGCGAGTGAAGCCGAAGCGCACCACATCCTTC
TTCAGCCGGCAGCTGTCCTTGGGCCAGGGGAGCTACACCGTGGTGCAGCCCGGCGACAGC
CTGGAGCAGGGCTGA

Clone variation with respect to NM_031904.3
339 c=>t;1121 c=>t
5' Read Nucleotide Sequence
>OriGene 5' read for NM_031904 unedited
TCCCCCGCCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCA
GAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGA
ATTCGGCACGAGGGCTGCGTCCTTAGCGGGAGCCCGAGTGCGGGCGGTGGCGGGCTTGGC
GGCGGGGCAGGATTCCAGGCAGGAGCCTTGCCTCTCAGGTGGCGGGCTCTGCGACCCTGC
GAGATGGACGGGACAGAAGGCAGTGCCGGGCAGCCCGGCCCCGCTGAGCGATCCCACCGA
AGCAGCGTGTCCTCCGTGGGAGCCCGAGCGGCTGACGTGCTGGTATACCTAGCGGATGAC
ACGGTGGTGCCCCTGGCTGTGGAGAACCTGCCCTCGCTCAGTGCCCATGAGCTGCACCGC
GCTGTCCGCGAGGTCCTGCAGCTTCCAGACATCGCCCTGGATGTCTTCGCGCTCTGGCTG
GTCTCCCCTCTGCTGGAGGTGCAGCTGAAACCCAAGCACCAGCCCTACAAGCTGGGACGC
CAGTGGCCGGAGCTGCTGCTGCGCTTCACCAGTGCCCCAGATGATGACGTGGCCATGGAT
GAGCCTTTCCTGCAGTTCCGAAGGAACGTGTTCTTCCCAAAGCGGCGGGAGCTCCAGATC
CATGACGAGGAGGTCCTGCGGCTGCTCTATGAGGAGGCCAAGGGCAACGTGCTGGCTGCA
CGGTACCCGTGCGACGTGGAGGACTGCGAGGCTCTGGGCGCCCTGGTGTGCCGCGTGCAG
CTTGGGCCCCTACAGCCGGCCGGGCCGCAGCCTGCGACCTGAGGGAGAAGCTGGNACTCT
TNCTCCCTGCCCACCTCTGTTAGCGGGGCCAGAGTCTCTTTGCTGCCCCTCGGGGCCGTG
GGCCAGGGCGGGCCCGGCGGCAGGGCCTGCTGAACGCTACCGCCAAGTGCAGAGTCAGCA
CGAC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_031904 unedited
TTTAAAACCCTTTNNTTTTTGTTAGAAACTGTTTTTAATAAGTCACATGATACAAAAGAA
TGAGAACATTCAAAGAATGAGTAAAATACTGCTTTGTCCCAAAGGACAAGCAGAAAATGT
TAAGGCACAACGGATGCTCAGAAAACGTAAGCAGCTGAAGGGAAAACACATCATCTGTGT
TCTTCTTACACACACACTCCAACCCATCACACGAACACACCCTCGCCCGCCCATCAGAGA
AGAATTCGCCTGGAATCAGCTGGGGGCGGTGGCTCACGCCTATAATCCCAGCACTTTGGG
AGGTTGAGGCGGGCAGATCATGAGGTCAGGAGTTCACGACCAGCCTGACCAACATGGTGA
AACCCTATCTCTACTAAAAATACAAAAATCAGCGGGGCCTGGTGGCATGCACCTGTAATC
CCAGCTACTCAGGAGGCTGAGGCAGGAGAATCGCTTGAGACAGAGGTTGCAGTGAGCCGA
GATGCGCCACTGCACTCCTGCCTGGGCAACAGAGCAAGACTCCGTCTCAAAAAAAAATAA
AAAAATAAAAAAAATAAAATCACCTGGAATCAAAGCAGCAGGGACAGCCTGCGCTGGAGC
CGCCAGAACAGGCAGGCGGGCAGCTCCGCAAGACCGAGCAACTCCAGNACGGGAGACGAG
GACTGGCAGCTGACATGACCCCTGTGACCAGGACATTTCCAAAGACTGGGGGGCCCCTCC
CCAAGCCTCTTTCTTTCCGCCCATTATTTTAACAAAATTCCATGCCTGGCCAGATGGCAA
GCCCTACCCTCGGAAACACCTGGGCCCCATTTCCCAAGCCTACCAAGAGGGAAGGAAGGG
TGGG
Restriction Sites NotI-NotI |
ACCN NM_031904
Insert Size 4000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_031904.2, NP_114110.1
RefSeq Size 3693 bp
RefSeq ORF 1395 bp
Locus ID 83786
UniProt ID Q9BZ67
Cytogenetics 11q13.1
Domains B41
Summary Promotes the cell surface stability of iRhom1/RHBDF1 and iRhom2/RHBDF2 and prevents their degradation via the endolysosomal pathway. By acting on iRhoms, involved in ADAM17-mediated shedding of TNF, amphiregulin/AREG, HBEGF and TGFA from the cell surface (PubMed:29897333, PubMed:29897336). Negatively regulates Wnt signaling, possibly by antagonizing the recruitment of AXIN1 to LRP6 (PubMed:19572019).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).
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Documents
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"NM_031904" in other vectors (4)
SKU Description Size Price
RC207879 FRMD8 (Myc-DDK-tagged)-Human FERM domain containing 8 (FRMD8) 10 ug
$457.00
RC207879L3 Lenti ORF clone of Human FERM domain containing 8 (FRMD8), Myc-DDK-tagged 10 ug
$757.00
RC207879L4 Lenti ORF clone of Human FERM domain containing 8 (FRMD8), mGFP tagged 10 ug
$757.00
RG207879 FRMD8 (tGFP-tagged) - Human FERM domain containing 8 (FRMD8) 10 ug
$489.00 $657.00

file-alt Citations

FRMD8 promotes inflammatory and growth factor signalling by stabilising the iRhom/ADAM17 sheddase complex
Künzel, Grieve, Meng et al
Elife (2018) 7 DOI: 10.7554/eLife.35012
AI Summary: In this study, OriGene's FRMD8 cDNA clone (Cat# RC208659) and FRMD8 shRNA plasmids (Cat# TL313648) were used to manipulate FRMD8 expression levels, enabling the authors to investigate its role in stabilizing the iRhom2/ADAM17 sheddase complex and regulating inflammatory and growth factor signalling.
FigureFigureFigure
FRMD8 promotes inflammatory and growth factor signalling by stabilising the iRhom/ADAM17 sheddase complex
Künzel, Grieve, Meng et al
bioRxiv (2018)
Snippet: onkey polyclonal Thermos Fisher Scientific Cat#A-31573;RRID:AB_2536183 dilution is described in materials and methods Recombinant DNA reagent cDNA: human iRhom2 (NM_024599.2) Origene Cat#SC122961 Recombinant DNA reagent cDNA: human FRMD8 (NM_031904) Origene Cat#SC107202 Recombinant DNA reagent cDNA: human UNC93B1 (Brinkmann et al., 2007) N/A;;Human UNC93B1, human iRhom2 WT , iRhom2 Δ 100 , iRhom2 Δ 200 , iRhom2 Δ 300 , iRhom2 Δ 382 392 were amplified from human UNC93B1 (BC025669.1) and iRhom2 cDNA (NM_024599.2;393 Origene ( SC122961)) by PCR and cloned with an C - terminal 3xHA tag into the lentiviral 394 vector pLEX.puro using Gibson assembly (New England Biolabs) following the 395 manufacturer’s instructions
Showing 1-2 of 2 papers.
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