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MRPL32 (NM_031903) Human Untagged Clone

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SKU
SC107191
MRPL32 (untagged)-Human mitochondrial ribosomal protein L32 (MRPL32), nuclear gene encoding mitochondrial protein
  $300.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol MRPL32
Synonyms bMRP-59b; HSPC283; L32mt; MRP-L32
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC107191 sequence for NM_031903 edited (data generated by NextGen Sequencing)
ATGGCGCTGGCCATGCTGGTCTTGGTGGTTTCGCCGTGGTCTGCGGCCCGGGGAGTGCTT
CGAAACTACTGGGAGCGACTGCTACGGAAGCTTCCGCAGAGCCGGCCGGGCTTTCCCAGT
CCTCCGTGGGGACCAGCATTAGCAGTACAGGGCCCAGCCATGTTTACAGAGCCAGCAAAT
GATACCAGTGGAAGTAAAGAGAATTCCAGCCTTTTGGACAGTATCTTTTGGATGGCAGCT
CCCAAAAATAGACGCACCATTGAAGTTAACCGGTGTAGGAGAAGAAATCCGCAGAAGCTT
ATTAAAGTTAANAACAACATAGACGTTTGTCCTGAATGTGGTCACCTGAAACAGAAACAT
GTCCTTTGTGCCTACTGCTATGAAAAGGTGTGCAAGGAGACTGCAGAAATCAGACGACAG
ATAGGGAAGCAAGAAGGGGGCCCTTTTAAGGCTCCCACCATAGAGACTGTGGTGCTGTAC
ACAGGAGAGACACCGTCTGAACAAGATCAGGGCAAGAGGATCATTGAACGAGACAGAAAG
CGACCATCCTGGTTCACCCAGAATTGA

Clone variation with respect to NM_031903.2
312 g=>n;483 g=>a
5' Read Nucleotide Sequence
>OriGene 5' read for NM_031903 unedited
AATTAAAAATTGAACACGACTCATATAGGCGGACGCGAATTGGACGAGGGGAAAAGGCGC
TGGCCATGCTGGTCTTGGTGGTTTCGCCGTGGTCTGCGGCCCGGGGAGTGCTTCGAACTA
CTGGGAGCGACTGCTACGGAAGCTTCCGCAGAGCCGGCCGGGCTTTCCCAGTCCTCCGTG
GGGACCAGCATTAGCAGTACAGGGCCCAGCCATGTTTACAGAGCCAGCAAATGATACCAG
TGGAAGTAAAGAGAATTCCAGCCTTTTGGACAGAATCTTTTGGATGGCAGCTCCCAAAAA
TAGACGCACCATTGAAGTTAACCGGCGTAGGAGAAGAAATCCGCAGAAGCCTTATTAAAG
TTAAGGTAATGCATTGATTTTTGTGGGTGTGCTTTTCCTCTAAGTCTCCATTGAATAAGC
TCATGGTCCCATCCTATAGCCTAGGAATAAATTACTTCTCAGACTATATGTGCCTACTTT
CCTCTGGCCTAAATGAAATACCAGTATTCTCATTTATATACATAATGAGAAGGCTAGAGC
CTGCTGTCCTTTAGCCTGAGTATTAGAAACACAGCCCAGGAAGGAGTGAGCTTGGAGACT
GGGACCGGAACCAGGCTGTGAAGTGCCTACATGACTTTACGAACTGAGAGCTGGGTCAAC
TTTGAGCTGTGTGAGGCCTGAACTACCGAAGAGATACCATCCTACCTATTGCACAACCTT
AAAATTAAGGGCTTGAAGCCGTAGGCTATCCTGAAACCTTTCGGTTTCCACACCCCCTTC
CCACCACCGGTCTAATTTGTTTAGCATTTTCTACACAAACACCATGTAAGTAGACCTCTT
ACACTAGTCCCTCTATTGCTTAACACTCAATACAGGGTCAAAAAGGGAAACTACAGGCGC
CTATGGGTTAGATAAACCACACCATAGCCTATAAGCCTCCCCAGCAAAAAAAACA
3' Read Nucleotide Sequence
>OriGene 3' read for NM_031903 unedited
CCCAAATCTTGNCCGCGGCCGCAATCTACGAGCCGCTTTTTTTTTTTTTTTTTTGCTATG
AATTTTAAATTTTTTATTTTTGTTCTGGAAATTTTATGTTTACTTAAAACCTTGTATATA
ATAGAAACATAATCCATTGAGCCTTTGGATAAACAAAATTTTCGGAGTTTACTCATAATA
TATTCTTCAAATTATCCCACACAAAACTGCTTAGAAAGAATGTGTTAAACTATACTGATG
ATTTAAAAACAAGCACAACAACGTAAAGAATCTTCCTCTATTTCAGGAGAAATGATTTAC
TGTGAAGTTATCCTTTTAACATCTTTGGTGTCAATTCTGGGTGAACCAGGATGGTCGCTT
TCTGTCTCGTTCAATGATCCTCTTGCCCTGATCTTGTTCAGACGGTGTCTCTCCTGTGTA
CAGCACCACAGTCTCTATGGTGGGAGCCTTAAAAGGGCCCCCTTCTTGCTTCCCTATCTG
TCGTCTGAATTCTGCAGTCTCCTTGCACACCTTTTCATAGCAGTAGGCACAAAGGACATG
TTTCTGTTTCAGGTGACCACATTCAGGACAAACGTCTATGTTGTTCTTTAAAACAATAAA
CAAAAACGTATTTTAACATGAGGCAATATAACGAACAAATAACCACTGACAGGAGCAATG
CTTACATTTTAACTTTCTTAGGATGTTCAGGCTACATGAGGTCATCATACACCATCCAGT
TTCACCTCACTTTAACAGGAAGACTGAAGACCAGCACACTGTGTTTGAATTAGAGAAAAC
TAGTACTAGAATCCGCCAACCTGAATCTGGTATCTTTATTAAACACAGCGCTGTCGTTAT
GTAAAAAAGAAAAAGAAAACCCTTTTCCCCTACTCCCAAACTAAAATCACAAAGGAAATT
GCCACCTAAGG
Restriction Sites NotI-NotI |
ACCN NM_031903
Insert Size 3110 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_031903.1, NP_114109.1
RefSeq Size 903 bp
RefSeq ORF 567 bp
Locus ID 64983
UniProt ID Q9BYC8
Cytogenetics 7p14.1
Summary Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L32 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome Xp. provided by RefSeq, Jul 2008
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"NM_031903" in other vectors (5)
SKU Description Size Price
RC204559 MRPL32 (Myc-DDK-tagged)-Human mitochondrial ribosomal protein L32 (MRPL32), nuclear gene encoding mitochondrial protein 10 ug
$300.00
RC204559L3 Lenti ORF clone of Human mitochondrial ribosomal protein L32 (MRPL32), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged 10 ug
$600.00
RC204559L4 Lenti ORF clone of Human mitochondrial ribosomal protein L32 (MRPL32), nuclear gene encoding mitochondrial protein, mGFP tagged 10 ug
$600.00
RG204559 MRPL32 (tGFP-tagged) - Human mitochondrial ribosomal protein L32 (MRPL32), nuclear gene encoding mitochondrial protein 10 ug
$500.00
SC322462 MRPL32 (untagged)-Human mitochondrial ribosomal protein L32 (MRPL32), nuclear gene encoding mitochondrial protein 10 ug
$300.00

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