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MAGED4B (NM_177535) Human Untagged Clone

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SKU
SC107031
MAGED4B (untagged)-Human melanoma antigen family D, 4B (MAGED4B), transcript variant 2
  $679.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol MAGED4B
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_177535, the custom clone sequence may differ by one or more nucleotides


ATGGCTGAGGGAAGCTTCAGCGTGCAATCGGAAAGCTACAGTGTTGAAGACATGGATGAGGGTAGCGACG
AAGTCGGGGAGGAAGAGATGGTTGAAGGCAACGACTATGAAGAATTCGGTGCGTTTGGTGGCTATGGCAC
CCTCACCAGCTTTGACATCCATATCCTCAGAGCCTTCGGAAGCTTGGGTCCAGGCCTTCGCATCTTATCG
AATGAGCCCTGGGAACTGGAAAACCCTGTGCTGGCCCAGACCCTGGTGGAGGCATTGCAGCTGGATCCGG
AAACACTTGCCAATGAGACGGCCGCCCGTGCTGCCAACGTAGCCCGCGCCGCCGCCTCCAACCGTGCGGC
TCGGGCCGCTGCCGCCGCTGCCCGTACCGCCTTCAGTCAGGTGGTCGCTAGCCACCGGGTGGCCACGCCG
CAGGTCTCAGGAGAGGATACCCAGCCCACGACCTACGCCGCCGAGGCTCAGGGGCCCACCCCTGAGCCAC
CCCTTGCTTCTCCGCAGACCTCCCAGATGTTAGTCACCAGTAAGATGGCTGCCCCCGAGGCTCCGGCAAC
CTCCGCACAGTCCCAGACAGGCTCCCCGGCCCAGGAGGCTGCTACTGAGGGCCCTAGTAGCGCCTGTGCT
TTCTCTCAGGCTCCGTGTGCCAGGGAGGTGGACGCCAACCGGCCCAGCACAGCCTTCCTGGGCCAGAATG
ATGTCTTCGATTTCACTCAGCCGGCAGGTGTCAGTGGCATGGCCTTCCCGCGCCCCAAGAGACCTGCCCC
AGCCCAAGAGGCTGCCACAGAGGGCCCCAGTGCTGCCTCTGGTGTGCCCCAGACGGGACCTGGCAGGGAG
GTGGCAGCCACCCGGCCCAAGACCACCAAGTCGGGGAAGGCGCTGGCCAAGACTCGGTGGGTGGAGCCTC
AGAATGTTGTGGCAGCAGCTGCTGCCAAGGCCAAGATGGCCACGAGCATCCCTGAGCCGGAGGGTGCAGC
TGCTGCCACTGCTCAGCACAGTGCTGAGCCCTGGGCCAGGATGGGAGGCAAGAGGACCAAGAAGTCCAAG
CACCTGGATGATGAGTATGAGAGCAGCGAGGAGGAGAGAGAGACTCCCGCGGTCCCACCCACCTGGAGAG
CATCACAGCCCTCATTGACGGTGCGGGCTCAGTTGGCCCCTCGGCCCCCGATGGCCCCGAGGTCCCAGAT
ACCCTCAAGGCACGTACTGTGCCTGCCCCCCCGCAACGTGACCCTTCTGCAGGAGAGGGCAAATAAGTTG
GTGAAATACCTGATGATTAAGGACTACAAGAAGATCCCCATCAAGCGCGCAGACATGCTGAAGGATGTCA
TCAGAGAATATGATGAACATTTCCCTGAGATCATTGAACGAGCAACGTACACCCTGGAAAAGAAGTTTGG
GATCCACCTGAAGGAGATCGACAAGGAAGAACACCTGTATATTCTTGTCTGCACACGGGACTCCTCAGCT
CGCCTCCTTGGAAAAACCAAGGACACTCCCAGGCTGAGTCTCCTCTTGGTGATTCTGGGCGTCATCTTCA
TGAATGGCAACCGTGCCAGCGAGGCTGTCCTCTGGGAGGCACTACGCAAGATGGGACTGCGCCCTGGGGT
GAGGCACCCATTCCTCGGCGATCTGAGGAAGCTCATCACAGATGACTTTGTGAAGCAGAAGTACCTGGAA
TACAAGAAGATCCCCAACAGCAACCCACCTGAGTATGAATTCCTCTGGGGCCTGCGAGCCCGCCATGAGA
CCAGCAAGATGAGGGTCCTGAGATTCATCGCCCAGAATCAGAACCGAGACCCCCGGGAATGGAAGGCTCA
TTTCTTGGAGGCTGTGGATGATGCTTTCAAGACAATGGATGTGGATATGGCCGAGGAACATGCCAGGGCC
CAGATGAGGGCCCAGATGAATATCGGGGATGAAGCGCTGATTGGACGGTGGAGCTGGGATGACATACAAG
TCGAGCTCCTGACCTGGGATGAGGACGGAGATTTTGGCGATGCCTGGGCCAGGATCCCCTTTGCTTTCTG
GGCCAGATACCATCAGTACATTCTGAATAGCAACCGTGCCAACAGGAGGGCCACGTGGAGAGCTGGCGTC
AGCAGTGGCACCAATGGAGGGGCCAGCACCAGCGTCCTAGATGGCCCCAGCACCAGCTCCACCATCCGGA
CCAGAAATGCTGCCAGAGCTGGCGCCAGCTTCTTCTCCTGGATCCAGCACCGTTGA

5' Read Nucleotide Sequence
>OriGene 5' read for NM_177535 unedited
CGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGTGAGGACGGACGCCATTGGAGTTC
CCGAGAAGCTGAGCTCTCATCTCCCTGGGACCCGCAGCATGGCTGAGGGAAGCTTCAGCG
TGCAATCGGAAAGCTACAGTGTTGAAGACATGGATGAGGGTAGCGACGAAGTCGGGGAGG
AAGAGATGGTTGAAGGCAACGACTATGAAGAATTCGGTGCGTTTGGTGGCTATGGCACCC
TCACCAGCTTTGACATCCATATCCTCAGAGCCTTCGGAAGCTTGGGTCCAGGCCTTCGCA
TCTTATCGAATGAGCCCTGGGAACTGGAAAACCCTGTGCTGGCCCAGACCCTGGTGGAGG
CATTGCAGCTGGATCCGGAAACACTTGCCAATGAGACGGCCGCCCGTGCTGCCAACGTAG
CCCGCGCCGCCGCCTCCAACCGTGCGGCTCGGGCCGCTGCCGCCGCTGCCCGTACCGCCT
TCAGTCAGGTGGTCGCTAGCCACCGGGTGGCCACGCCGCAGGTCTCAGGAGAGGATACCC
AGCCCACGACCTACGCCGCCGAGGCTCAGGGGCCCACCCCTGAGCCACCCCTTGCTTCTC
CGCAGACCTCCCAGATGTTAGTCACCAGTAAGATGGCTGCCCCCNGAGCTCCGGCAACCT
CCGCACAGTCCCAGACAGGCTCCCCGGCCCAGGAGGCTTGCTACTGAGGGCCCTAGTAGC
GCCTGTGCTTCTCTCAGCTTCGTGGCAAAGGGGAGGTGGACGCCAACCGGCCCACACAGC
CTTCCTGGCCANAATGATGTCTTCGATTTACTCNNCGGAAGGGTGTCANTGGCATGCNCT
TNCCGCGCCCNAGAGACTGNCCAGNCCAGAGCTGCACAGAGGGGCCATGCTTCTCTTTGG
GTGCCCAAACGACTGNGGGGGGGAAGGGGCANCACCCGCCAGACACGTTGGGGGAAGGCT
GCA
Restriction Sites NotI-NotI |
ACCN NM_177535
Insert Size 2500 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_177535.1, NP_803879.1
RefSeq Size 2573 bp
RefSeq ORF 2226 bp
Locus ID 81557
UniProt ID Q96JG8
Cytogenetics Xp11.22
Summary This gene is a member of the MAGED gene family. It is expressed only in brain and ovary, and some transcript variants of this gene are specifically expressed in glioma cells. This gene is clustered with other MAGED genes on chromosome Xp11. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. provided by RefSeq, May 2011
Transcript Variant: This variant (2, also known as MAGE-E1a) has a slightly shorter 5' UTR compared to variant 1, as a result of using an alternate acceptor splice site at the 2nd exon. Variants 1 and 2 encode the same isoform (1).
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Documents
Resources
"NM_177535" in other vectors (4)
SKU Description Size Price
RC212260 MAGED4B (Myc-DDK-tagged)-Human melanoma antigen family D, 4B (MAGED4B), transcript variant 2 10 ug
$679.00
RC212260L3 Lenti ORF clone of Human melanoma antigen family D, 4B (MAGED4B), transcript variant 2, Myc-DDK-tagged 10 ug
$979.00
RC212260L4 Lenti ORF clone of Human melanoma antigen family D, 4B (MAGED4B), transcript variant 2, mGFP tagged 10 ug
$979.00
RG212260 MAGED4B (tGFP-tagged) - Human melanoma antigen family D, 4B (MAGED4B), transcript variant 2 10 ug
$879.00

file-alt Citations

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