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WDR54 (NM_032118) Human Untagged Clone

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SKU
SC106524
WDR54 (untagged)-Human WD repeat domain 54 (WDR54)
  $457.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol WDR54
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC106524 sequence for NM_032118 edited (data generated by NextGen Sequencing)
ATGTTCCGCTGGGAGCGCTCCATTCCCCTGCGAGGCTCGGCCGCCGCCCTGTGCAACAAC
CTCAGTGTGCTGCAGCTGCCGGCTCGCAACCTCACGTATTTTGGCGTGGTTCATGGACCA
AGCGCCCAGCTTCTCAGCGCTGCTCCTGAGGGTGTGCCCTTGGCCCAGCGCCAGCTCCAC
GCTAAGGAGGGTGCTGGAGTGAGTCCCCCACTTATCACTCAGGTCCACTGGTGTGTCCTC
CCCTTCCGAGTGCTGCTGGTACTCACCTCACATCGAGGAATACAGATGTACGAGTCCAAT
GGCTACACCATGGTCTACTGGCATGCACTGGACTCTGGAGATGCCTCCCCAGTACAGGCT
GTGTTTGCCCGGGGAATTGCTGCCAGTGGCCACTTCATCTGTGTGGGAACGTGGTCAGGC
CGGGTGCTGGTGTTTGACATCCCAGCAAAGGGTCCCAACATTGTACTGAGCGAGGAGCTG
GCTGGGCACCAGATGCCAATCACAGACATTGCCACCGAGCCTGCCCAGGGACAGGATTGT
GTGGCTGACATGGTGACGGCAGATGACTCAGGCTTGCTGTGTGTCTGGCGGTCAGGGCCA
GAATTCACATTATTGACCCGCATTCCAGGATTTGGAGTTCCGTGCCCCTCTGTGCAGCTG
TGGCAGGGGATCATAGCAGCAGGCTATGGGAACGGACAAGTGCATCTATATGAGGCCACT
ACAGGAAATCTACATGTCCAGATCAATGCCCATGCCCGGGCCATCTGCGCCCTGGACCTG
GCTTCTGAGGTGGGCAAGCTACTCTCTGCAGGTGAGGACACCTTTGTGCATATCTGGAAG
CTGAGCAGAAACCCAGAGAGTGGCTACATTGAGGTGGAACACTGTCATGGTGAGTGTGTC
GCCGACACCCAGCTGTGTGGTGCTCGATTTTGTGATTCCTCAGGCAACTCCTTTGCTGTG
ACTGGCTATGACCTTGCGGAGATCCGGAGATTCAGCAGTGTGTGA

Clone variation with respect to NM_032118.2
5' Read Nucleotide Sequence
>OriGene 5' read for NM_032118 unedited
NGGTGTCAAAAATTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGGATTT
GGAGGGACCCTACGAACCAGGAGTCAGGCGAGCCGATCTGGGGCTGCAGGATGTTCCGCT
GGGAGCGCTCCATTCCCCTGCGAGGCTCGGCCGCCGCCCTGTGCAACAACCTCAGTGTGC
TGCAGCTGCCGGCTCGCAACCTCACGTATTTTGGCGTGGTTCATGGACCAAGCGCCCAGC
TTCTCAGCGCTGCTCCTGAGGGTGTGCCCTTGGCCCAGCGCCAGCTCCACGCTAAGGAGG
GTGCTGGAGTGAGTCCCCCACTTATCACTCAGGTCCACTGGTGTGTCCTCCCCTTCCGAG
TGCTGCTGGTACTCACCTCACATCGAGGAATACAGATGTACGAGTCCAATGGCTACACCA
TGGTCTACTGGCATGCACTGGACTCTGGAGATGCCTCCCCAGTACAGGCTGTGTTTGCCC
GGGGAATTGCTGCCAGTGGCCACTTCATCTGTGTGGGAACGTGGTCAGGCCGGGTGCTGG
TGTTTGACATCCCAGCAAAGGGTCCCAACATTGTACTGAGCGAGGAGCTGGCTGGGCACC
AGATGCCAATCACAGACATTGCCACCGAGCCTGCCCAGGGACAGGATTGTGTGGCTGACA
TGGTGACGGCAGATGACTCANGCTTGCTGTGTGTCTGGCGGTCANGGCCAGAATTCACAT
TATTGACCCGCATTCCAGGATTTGGAGTTCCGTGCCCCTCTGTGCAGCTGTGGCAGGNGA
TCATAGCAGCAGGCTATGGGACGGNACAAGTGCATCTATATGAGGCCACTACAGGAAATC
TACATGTCCAGATCAATGCCATGCCGGNCCATCTGCGCCCTGGACCTGCTTNCTGAGTGG
CAAGCTACTCTNCTGCAGTGAGACACCTTGTGCATAC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_032118 unedited
TATGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTGGGGGGGGCGGGGACTTTTTTAATACCCCAGGGACCAAGGAAG
GGTGCTTTTTTCACACACTGGTGAATCTCCGGATCTCCCCAAGGGCATAACCCGTCACAA
CAAAGGAGTTGCCTGAGGAATCACAAAATCCAGCCCCCCACCGCTGGGGGGTGGCGACAC
ACTCACCATGACAGGGGTCCACCTCAATGGAACCAATTTTTGGGGTTCTGCTCAACTTCC
AAAAATGCACAAAGGGGGCCTCACCTGCAAAAAGGAACTTGCCCACCTCAAAAACCAAGT
CCAGGGCGCAAATGGCCCCGGCATGGGCCTTGATCTGGACATGTAAATTTCCTGGAGGGG
CCTCATATAGAAGCACTTGTCCGGTCCCATAACCTGGTGGTTTGATCCCCTGCCACAACT
GGACAGAGGGGCACGGAACTCCAAAACCTGGAATGGGGGGCAATAATGTGAATTCTGGCC
CTGACCGGCAAACACACAAGAAGCCCTGAGTCATCTGGCGTCACCATGTAAACCACACAA
TCCTTGTCCTGGGCAAGCTTNGTGGGAATGTCTGTGATTGGATTTGGTGCCCAACCAACT
TCTTTTTAAAACAAGTTGGGACCCTTTGTTGGAAGTAAAACACAAAACCGGGCTTGACAC
GTTCCCAAACAAGAAGGGGCCCTGGCAGAATTCCCCCGGGAAACCAGCCTGTGTGGGGAG
GCTTTTCCAAGCCACGGTTGCCAATAACACCTGGGACCCCCTGACTCCCCCTTTGTTTTC
CCGAGGGGGGGGGCCCACCCCCCTCGGAGGAGGGACCCCCCGGCCCTGAGGAAAGGGGGA
CCCCCCACCCCTCCTTAGGGAGGGGGGCGGCCAAGGACCCCTTAGAACACTG
Restriction Sites NotI-NotI |
ACCN NM_032118
Insert Size 1220 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032118.2, NP_115494.1
RefSeq Size 1223 bp
RefSeq ORF 1005 bp
Locus ID 84058
UniProt ID Q9H977
Cytogenetics 2p13.1
Domains WD40
Summary When cross-linked to form dimers and trimers, it has a regulatory effect on ERK signaling pathway activity in response to EGF stimulation. Colocalizes with the EGF receptor in WDR54-specific vesicle where it sustains the internalization and controls the degradation of the EGF receptor after EGF stimulation.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (3) uses an alternate splice junction compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 2 and 3 both encode the same isoform (b).
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Documents
Resources
"NM_032118" in other vectors (4)
SKU Description Size Price
RC208682 WDR54 (Myc-DDK-tagged)-Human WD repeat domain 54 (WDR54) 10 ug
$457.00
RC208682L3 Lenti ORF clone of Human WD repeat domain 54 (WDR54), Myc-DDK-tagged 10 ug
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RC208682L4 Lenti ORF clone of Human WD repeat domain 54 (WDR54), mGFP tagged 10 ug
$757.00
RG208682 WDR54 (tGFP-tagged) - Human WD repeat domain 54 (WDR54) 10 ug
$489.00 $657.00

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