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ZNRF3 (NM_032173) Human Untagged Clone

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SKU
SC106136
ZNRF3 (untagged)-Human zinc and ring finger 3 (ZNRF3), transcript variant 2
  $150.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol ZNRF3
Synonyms BK747E2.3; RNF203
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>SC106136 representing NM_032173.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

ATGTACTTTTGTGGCCTTGGTGTCCGATGGGGCTGGGGGAGAGTGCTCTCCACTGACCCAGCAGCACAC
CCATGTGCAGTGCGCCTGCATCTGTGTGGGGGCAGCCACACCCCTTGGCTGCTGCTTCCTTGGGCTGCC
TTTCTGGGGGCATGTGACTGGACCTACGAGGTCTGCACTGAGCTCCATTTGAATGATACCTTTCCTATC
CCATTTCCCCCACGGAAGCACCGCTTCAGGGTTATTCAGTCCTCTGCCTCATGGCTGAAATTGCTCATC
TCGTCTGCAGATGTCTACTATCCTGTCTACCTAATGCACTATTATGTATTGATTCTCCATGAGACAGAG
AGAGAGAGAGACTATCAGATAGTTTACACCCAAAGGGTAGGTTTTTGTATATTTTTCCAGCCTTTTTTA
TTAAGGGGAAGGGGAGAGTTTAAAAACCCAAACCGTTGTGGTTTTAAGGTGTTTCATTTTTAA
5' Read Nucleotide Sequence
>OriGene 5' read for NM_032173 unedited
GGTCACCATTTGTATACGACTCATATGGGCGGCCGCGCAATTCGCACGAGGCTCTGAGGA
GCAGTGCAAAACTGCCTCTTTCTTTCTCGTGGCAAATCCCAATGTACACGATTTCAGGTC
TCAGACGCCATGCCTCTCCAGCCCACGCCTTTAGGCAGGTGATGGCAGCAGCTAGGAATA
GGGTGTACATGATCCACAGCCCTGCGGAGCCAGGTCAAGCCGCTGCTATGAAAGCTCCAG
GGTGATGGGGACGATTCTGCCCAGTGTCCTCAGTCTGTCCCCTCAGGTCATGGTCCCAAG
TGAAATGACAGAGTTCACAGCCCTGGTCTTGGCTGAGGTCCAGGTCATAGTAAGGGCATG
TTCTTGGGGCCCTCGACCTGAACTCTGACCCTCCGGGCAGGGAAGAGGAGGTTGTCCCCT
TTGGTTGTCCTGGCTTTGGAGTCCTTTGCAAAAATATTTTGGGCCCCCTGCCACTGGCTG
CAGAAATGGCTCGACGGGGTGTGTGGGGACAGACACCCAGAAGGAATGTACTTTTGTGGC
CTTGGTGTCCGATGGGGCTGGGGGAGAGTGCTCTCCACTGACCCAGCAGCACACCCATGT
GCAGTGCGCCTGCATCTGTGTGGGGGCAGCCACACCCCTTGGCTGCTGCTTCCTTGGGCT
GCCTTTCTGGGGGCATGTGACTGGACCTACGAGGTCTGCACTGAGCTCCATTTGCATGAT
ACCTTTCCTATCCCATTTCCCCACGGAAGCACCGCTTCAGGGTTTATCAGTCCTCTGCCT
CATGCCTGCAATTGCTCATCTCGTCTGCAGATGTCTACTATCCTGTCTACCTAATGCACT
ATAATGTATTGATTCTTCCTGAGACCGAGAGAGAGAGAGACTATCCGAAGTTTCACCCCA
AGGTAGGGTTTTGATACTTTTCCGCCT
3' Read Nucleotide Sequence
>OriGene 3' read for NM_032173 unedited
TTTGGCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGTTTTTTTTTTAA
CACATACTTGATAAAATTGTATTTTTTTTTTACAGTCATGTGTACAATTTGTTACAAAAC
CATAGAAGACTACAACTTGTTTTAAATCATTTTTGGTCTGCAAATATGTAAAATCTGTGT
GCAATTATCATGTATTTACAGGGTCTTGTGTTAGTCATTTTCAATGATTATTCCAACAAT
GTCACACTCTCAACATAAGACATGGCTTAAGATAAATATATTAGTAAATAAATATTCTGA
GAACATATTTCCATAAATGAAATGTGCTGCTATACATATACAGAATATACATAAGTTGTC
TTCTAGCTTTTAAAACATTTTTTAAAAATGGTAATGTTGGAAAAAGAGCCCTTAAACCAT
TTTATTACAAAATCTTTACAGCAAGGTCTTTACAAAATCTCTTTTAAGTGCTATGGGAAA
AATTAAAAAATTTTAAAATAGTGCCTTGTTAGACCAACACAATAGAAAGAGTTTACCTAA
AGAGTAGAAAAAGGCAGTTCTGTTTAAAGAATAATAGTCTCGACTAGTAATTATTTTTTA
GACCTCATTTTAAAAGCATTAGTCCTGCACGAACTTCGAGTAGAGACTAGCAGCACATAA
GTATGTGAGCAGTGGGACAGAAGGGGCTTTATTTTATAAAAGGGCTTGGATTANGAGGAG
ACAGGGAAGGAGCTGAGAGTTAAAGCCAGCTTACTGGGGCTGCATCCATAAAGAGGTACC
TTGTTGGTAAACACCCAAACTTCCATTTTTAACTGGTTCTAGAACTCTTCACTGTATATG
GCTGCCCTACAACCAGGGCTGGGTCACTTGACCCTGCCCAATGCCACCCCCACTTANAGG
GATTGGGGGCAGGGCTCTCAAGGAAATGAAAACTCCTATTTCCATGCTTTNAACCCCCCC
ACCTCCAATCCACGAAAGGG
Restriction Sites NotI-NotI |
ACCN NM_032173
Insert Size 3300 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032173.1
RefSeq Size 6489 bp
RefSeq ORF 477 bp
Locus ID 84133
UniProt ID Q9ULT6
Cytogenetics 22q12.1
Protein Families Druggable Genome, Transmembrane
MW 18.6 kDa
Summary E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination and subsequent degradation of Wnt receptor complex components Frizzled and LRP6. Acts on both canonical and non-canonical Wnt signaling pathway. Acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway, thereby resticting the size of the intestinal stem cell zone (PubMed:22575959). Along with RSPO2 and RNF43, constitutes a master switch that governs limb specification (By similarity).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_032173" in other vectors (7)
SKU Description Size Price
RC219546 ZNRF3 (Myc-DDK-tagged)-Human zinc and ring finger 3 (ZNRF3), transcript variant 2 10 ug
$765.00
RC219546L1 Lenti ORF clone of Human zinc and ring finger 3 (ZNRF3), transcript variant 2, Myc-DDK-tagged 10 ug
$1,065.00
RC219546L2 Lenti ORF clone of Human zinc and ring finger 3 (ZNRF3), transcript variant 2, mGFP tagged 10 ug
$1,065.00
RC219546L3 Lenti ORF clone of Human zinc and ring finger 3 (ZNRF3), transcript variant 2, Myc-DDK-tagged 10 ug
$1,065.00
RC219546L4 Lenti ORF clone of Human zinc and ring finger 3 (ZNRF3), transcript variant 2, mGFP tagged 10 ug
$1,065.00
RG219546 ZNRF3 (tGFP-tagged) - Human zinc and ring finger 3 (ZNRF3), transcript variant 2 10 ug
$789.00 $965.00
SC318318 ZNRF3 (untagged)-Human zinc and ring finger 3 (ZNRF3), transcript variant 2 10 ug
$843.00

file-alt Citations

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