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PPAPDC1B (PLPP5) (NM_032483) Human Untagged Clone

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SKU
SC104858
PPAPDC1B (untagged)-Human phosphatidic acid phosphatase type 2 domain containing 1B (PPAPDC1B), transcript variant 2
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PPAPDC1B
Synonyms DPPL1; HTPAP; PPAPDC1B
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_032483, the custom clone sequence may differ by one or more nucleotides


ATGGGGAAGGCGGCGGCGGCGGTGGCCTTTGGGGCCGAAGTGGGCGTGCGGCTCGCGCTGTTCGCGGCCT
TCCTGGTGACGGAGCTGCTCCCCCCGTTCCAGAGACTCATCCAGCCGGAGGAGATGTGGCTCTACCGGAA
CCCCTACGTGGAGGCGGAGTATTTCCCCACCAAGCCGATGTTTGTTATTGCATTTCTCTCTCCACTGTCT
CTGATCTTCCTGGCCAAATTTCTCAAGAAGGCAGACACAAGAGACAGCAGACAAGCCTGCCTGGCTGCCA
GCCTTGCCCTGGCTCTGAATGGCGTCTTTACCAACACAATAAAACTGATCGTAGGGAGGCCACGCCCAGA
TTTCTTCTACCGCTGCTTCCCTGATGGGCTAGCCCATTCTGACTTGATGTGTACAGGGGATAAGGACGTG
GTGAATGAGGGCCGAAAGAGCTTCCCCAGTGGACATTCTTCCTTTGCATTTGCTGGTCTGGCCTTTGCGT
CCTTCTACCTGGCAGGGAAGTTACACTGCTTCACACCACAAGGCCGTGGGAAATCTTGGAGGTTCTGTGC
CTTTCTGTCACCTCTACTTTTTGCAGCTGTGATTGCACTGTCCCGCACATGTGACTACAAGCATCACTGG
CAAGGACCCTTTAAATGGTGA

3' Read Nucleotide Sequence
>OriGene 3' read for NM_032483 unedited
CTTTGGACCGCGGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGAGAGGGGTC
TCACTCTGTTGCCTAGGCTGGAGTGCAGTGGTGCGATCTCGGCCCACTGGAACCTCCAGC
TCCCAGGTTCAAGCAATTCTCCTACCTCAGCCTCCCAGGTAGCTGGGATTACGGCACACA
ACTCCTGGCTAATTTTTGTATTTTTAGTAGAGACAGGGTTCACCATGTTGGCCAGGCTGG
TCCTGACCTCAGGTGATCCACCCTCCTCAGCCTCCCAAAGTGCTGGGATTACAGGCATGA
GCCACCACGCCCGGCCAGATTCAATTTTTAAATATCAAAACAATAAGAATCCCCAGGCTT
CTGTGCAGTGGAAAGTACAAGTTTGTCTTGAAATGGTTTATGGCATTCTGCATCAGTCAG
AGGAGGATAATACTGCCGATAGCAGACATAGGCAAATGTCATTCCAATCATGGATCCAAC
TAGTACATCTTGCCAGTGATGCTTGTAGTCACATGTGCGGGACAGTGCAATCACAGCTGC
AAAAAGTAGAGGTGACAGAAAGGCACAGAACCTCCAAGATTTCCCACGGCCTTGTGGTGT
GAAGCAGTGTAACTTCCCTGCCAGGTAGAAGGACGCAAAGGCCAGACCAGCANATGCAAA
GGAAGAATGTCCACTGGGGAAGCTCTTTCGGCCCTCATTCACCACGTCCTTATCCCCTGT
ACACATCAAGTCAGAATGGGCTAGCCCATCAGGNAAAGCAGCGTAGAAGAAATCTGGGCG
TGGCCTGGAAGAAACAGCATGGNTGGAAACGTAATCATTAACTNCAGAAATATTTNTTCA
GTGTAGCAGACATAAATATCCTGTTAACTATTGGTCAAATAGTGCCCCCAGCAAATAGGT
ACTGGCTTATATAGCCCCACACTAGAGAAAACCTTTTCAGTGAGCATTTAANTAAGGGAA
AACCCCATCCC
Restriction Sites NotI-NotI |
ACCN NM_032483
Insert Size 2140 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032483.2, NP_115872.1
RefSeq Size 880 bp
RefSeq ORF 528 bp
Locus ID 84513
UniProt ID Q8NEB5
Cytogenetics 8p11.23
Domains acidPPc
Protein Families Transmembrane
Summary Displays magnesium-independent phosphatidate phosphatase activity in vitro. Catalyzes the conversion of phosphatidic acid to diacylglycerol. May be a metastatic suppressor for hepatocellular carcinoma.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (2) has a different exon a the 3' terminus, compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus, compared to isoform 1.
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Documents
Resources
"NM_032483" in other vectors (5)
SKU Description Size Price
RC210619 PPAPDC1B (Myc-DDK-tagged)-Human phosphatidic acid phosphatase type 2 domain containing 1B (PPAPDC1B), transcript variant 2 10 ug
$300.00
RC210619L3 Lenti ORF clone of Human phosphatidic acid phosphatase type 2 domain containing 1B (PPAPDC1B), transcript variant 2, Myc-DDK-tagged 10 ug
$600.00
RC210619L4 Lenti ORF clone of Human phosphatidic acid phosphatase type 2 domain containing 1B (PPAPDC1B), transcript variant 2, mGFP tagged 10 ug
$600.00
RG210619 PPAPDC1B (tGFP-tagged) - Human phosphatidic acid phosphatase type 2 domain containing 1B (PPAPDC1B), transcript variant 2 10 ug
$489.00 $500.00
SC317319 PPAPDC1B (untagged)-Human phosphatidic acid phosphatase type 2 domain containing 1B (PPAPDC1B), transcript variant 2 10 ug
$330.00

file-alt Citations

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