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DEDD (NM_032998) Human Untagged Clone

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SKU
SC101980
DEDD (untagged)-Human death effector domain containing (DEDD), transcript variant 1
  $300.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol DEDD
Synonyms CASP8IP1; DEDD1; DEFT; FLDED1; KE05
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_032998, the custom clone sequence may differ by one or more nucleotides


ATGGCGGGCCTAAAGCGGCGGGCAAGCCAGGTGTGGCCAGAAGAGCATGGTGAGCAGGAACATGGGCTGT
ACAGCCTGCACCGCATGTTTGACATCGTGGGCACTCATCTGACACACAGAGATGTGCGCGTGCTTTCTTT
CCTCTTTGTTGATGTCATTGATGACCACGAGCGTGGACTCATCCGAAATGGACGTGACTTCTTATTGGCA
CTGGAGCGCCAGGGCCGCTGTGATGAAAGTAACTTTCGCCAGGTGCTGCAGCTGCTGCGCATCATCACTC
GCCACGACCTGCTGCCCTACGTCACCCTCAAGAGGAGACGGGCTGTGTGCCCTGATCTTGTAGACAAGTA
TCTGGAGGAGACATCAATTCGCTATGTGACCCCCAGAGCCCTCAGTGATCCAGAACCAAGGCCTCCCCAG
CCCTCTAAAACAGTGCCTCCCCACTATCCTGTGGTGTGTTGCCCCACTTCGGGTCCTCAGATGTGTAGCA
AGCGGCCAGCCCGAGGGAGAGCCACACTTGGGAGCCAGCGAAAACGCCGGAAGTCAGTGACACCAGATCC
CAAGGAGAAGCAGACATGTGACATCAGACTGCGGGTTCGGGCTGAATACTGCCAGCATGAGACTGCTCTG
CAGGGCAATGTCTTCTCTAACAAGCAGGACCCACTTGAGCGCCAGTTTGAGCGCTTTAACCAGGCCAACA
CCATCCTCAAGTCCCGGGACCTGGGCTCCATCATCTGTGACATCAAGTTCTCTGAGCTCACCTACCTCGA
TGCATTCTGGCGTGACTACATCAATGGCTCTTTATTAGAGGCACTTAAAGGTGTCTTCATCACAGACTCC
CTCAAGCAAGCTGTGGGCCATGAAGCCATCAAGCTGCTGGTAAATGTAGACGAGGAGGACTATGAGCTGG
GCCGACAGAAACTCCTGAGGAACTTGATGCTGCAAGCATTGCCCTGA

5' Read Nucleotide Sequence
>OriGene 5' read for NM_032998 unedited
ATTATGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGATGGATCCAACA
TGGCGGCGCCGAGCCTGAGCCGAGAGAAGAGACCTGGGAAATTAAGTTTCTTGCGGAGTA
CGGTGGCGATTGCAGCTGCTGAGCAGGGATTCTGGAAAGCATTGCGTACCTGAGCCCCCA
GCATGGCGGGCCTAAAGCGGCGGGCAAGCCAGGTGTGGCCAGAAGAGCATGGTGAGCAGG
AACACGGGCTGTACAGCCTGCACCGCATGTTTGACATCGTGGGCACTCATCTGACACACA
GAGATGTGCGCGTGCTTTCTTTCCTCTTTGTTGATGTCATTGATGACCACGAGCGTGGAC
TCATCCGAAATGGACGTGACTTCTTATTGGCACTGGAGCGCCAGGGCCGCTGTGATGAAA
GTAACTTTCGCCAGGTGCTGCAGCTGCTGCGCATCATCACTCGCCACGACCTGCTGCCCT
ACGTCACCCTCAAGAGGAGACGGGCTGTGTGCCCTGATCTTGTAGACAAGTATCTGGAGG
AGACATCAATTCGCTATGTGACCCCCAGAGCCCTCAGTGATCCAGAACCAAGGCCTCCCC
AGCCCTCTAAAACAGTGCCTCCCCACTATCCTGTGGTGTGTTGCCCCACTTCGGGTCCTC
AGATGTGTAGCAAGCGGCCAGCCCGAGGGAGAGCCACACTTGGGAGCCAGCGAAAACGCC
GGAAGTCAGTGACACCAGATCCCAAGGAGAAGCAGACATGTGACATCAGACTGCGGGTTC
GGGCTGAATACTGCCAGCATGAGACTGCTCTGCAGGGCAATGTCTTTCTCTAACAGCAGG
ACCCACTTGAGCGCCAGTTTGAGCGCTTTAACCAGCCCACACATNNCTCAGTCCCGGGAC
CTGGGCTCCATCATCTGTGACATCAAGNNTCTCTGAGCTACCTACTCGAGCATTCTGCGT
GACTACATCATGGCTCTTATTAGAGCACTTNAAGG
3' Read Nucleotide Sequence
>OriGene 3' read for NM_032998 unedited
GGGGNGGGGGGGNNNNNNNNNNNNTNNNNNNNNNAANNTTTTACTNTGNACCGCGGNCCG
CATACNAGNGATCGGTTTTTTTTTTTTTTTTTTTATAGTCATTGTTTATTTCATGGAAAC
TGAAGTTCTGCTGAGGGCTGAGCAGCACTGGCATTGAAAAAATAATAATCATAAAGTCTG
TGTCTGGACATCGCCTTTGGGAACTAGAAGGGGAGTTGGTATTGTACCAGCTGGACTAAG
CTCCAGTTCTAGACCTCCTGGCTCATTCAACATGCCTCCCTACCTAAATAAAAGTGCAAC
ACTCAGTGCATGTCCCAGCCCCATTCTCCCAAGCATGGGAGTGGGCGTAGGAGTGGAGGA
GGGGGAAGGAAAAAGGAATTACTTCACTTACACCTATGATGCCCTTTGCCCAAGCCAGAA
GAAAGCAAAGGGGAAAAGGGCTGCAGGGTACATTATTTATTTTCACTTGAACATGGAAAG
AAAGTGTCACACTCCCCCTTCCCCTTTATAGGGGGAGTGTATTTTAATCAGCAACCTCTT
CTCCATCCACCCTGTGTATGTGTGTACACATATACCACCACAGTTGGGAGGCATGACTAG
GCTGACCCAGTCTTATCTGTTCCTCCTTTGAAGAGGGTTTGACAAATGGGAAAAAGGAAG
GAGCCACATCANGCAGAGGTTTCAAGCCATGGAATGGANGAAAAGCNGCANAGAGGAGCA
NCACCAGAGGCCAGGAGAGAGTGGAAAAGGCCACAGCTNGNTTTTAAAAAATNCTATNAT
TNGGAAGAGGGNTGCGGATAATTTTCAAATACACTTCAGAGTCCACTTTCACATAGCTCC
CTTACTACAGNCCTTTGGTTNTTTAGACAAATTTAAGAGCTCTCATAAGCCAGAAGTATT
GATAACTCCTAGTGCATCCAAAAAAAGGGTTCACATCATCCCTACAAAGACTTGTGCTGT
AAACTTTGCTCCTT
Restriction Sites NotI-NotI |
ACCN NM_032998
Insert Size 2100 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032998.1, NP_127491.1
RefSeq Size 2261 bp
RefSeq ORF 957 bp
Locus ID 9191
UniProt ID O75618
Cytogenetics 1q23.3
Protein Families Druggable Genome, Transcription Factors
Summary This gene encodes a protein that contains a death effector domain (DED). DED is a protein-protein interaction domain shared by adaptors, regulators and executors of the programmed cell death pathway. Overexpression of this gene was shown to induce weak apoptosis. Upon stimulation, this protein was found to translocate from cytoplasm to nucleus and colocalize with UBTF, a basal factor required for RNA polymerase I transcription, in the nucleolus. At least three transcript variants encoding the same protein have been found for this gene. provided by RefSeq, Jul 2008
Transcript Variant: This variant (1) uses an alternate in-frame splice junction compared to variant 5. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. Variants 1, 3, and 4 all encode the same isoform (b).
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Documents
Resources
"NM_032998" in other vectors (6)
SKU Description Size Price
RC212926 DEDD (Myc-DDK-tagged)-Human death effector domain containing (DEDD), transcript variant 1 10 ug
$300.00
RC212926L1 Lenti ORF clone of Human death effector domain containing (DEDD), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC212926L2 Lenti ORF clone of Human death effector domain containing (DEDD), transcript variant 1, mGFP tagged 10 ug
$600.00
RC212926L3 Lenti ORF clone of Human death effector domain containing (DEDD), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC212926L4 Lenti ORF clone of Human death effector domain containing (DEDD), transcript variant 1, mGFP tagged 10 ug
$600.00
RG212926 DEDD (tGFP-tagged) - Human death effector domain containing (DEDD), transcript variant 1 10 ug
$489.00 $500.00

file-alt Citations

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