DCP2 (NM_152624) Human Untagged Clone

SKU SC100774

DCP2 (untagged)-Human DCP2 decapping enzyme homolog (S. cerevisiae) (DCP2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: DCP2
• Synonyms: NUDT20
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_152624, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGAGACCAAACGGGTGGAGATTCCCGGCAGCGTCCTGGACGATCTCTGCAGCCGATTTATTTTGCATA; TTCCCAGCGAGGAAAGAGACAATGCAATCCGAGTGTGTTTTCAGATTGAACTTGCCCATTGGTTTTACTT; GGATTTCTACATGCAGAACACACCAGGATTACCTCAGTGTGGGATAAGAGACTTTGCTAAAGCTGTCTTC; AGTCATTGTCCGTTTTTGCTGCCTCAAGGTGAAGATGTGGAAAAAGTTTTGGATGAATGGAAGGAATATA; AAATGGGAGTACCAACATATGGTGCAATTATTCTTGATGAGACACTTGAAAATGTACTACTAGTTCAGGG; GTACCTAGCAAAATCAGGCTGGGGATTTCCAAAAGGAAAAGTAAATAAAGAAGAAGCTCCTCATGATTGT; GCTGCTAGAGAGGTCTTTGAAGAAACTGGTTTTGATATCAAAGACTATATTTGTAAGGATGATTACATTG; AACTTCGAATCAATGACCAGCTTGCTCGTTTGTACATCATTCCAGGAATTCCAAAAGACACAAAATTTAA; CCCAAAAACTAGAAGAGAAATTCGGAACATTGAGTGGTTCTCTATTGAGAAATTGCCTTGTCATAGAAAT; GATATGACCCCCAAATCCAAACTTGGTTTGGCACCTAACAAATTTTTTATGGCCATTCCCTTTATCAGAC; CATTAAGGGACTGGCTTTCTCGAAGATTTGGCGATTCCTCAGACAGTGACAATGGATTTTCCTCAACTGG; TAGCACGCCGGCTAAACCCACTGTGGAAAAATTGAGTCGAACCAAATTCCGCCACAGTCAGCAGTTATTT; CCTGACGGTTCTCCTGGTGACCAGTGGGTAAAGCACAGGCAACCACTGCAGCAAAAGCCATATAATAATC; ATTCTGAAATGTCTGACCTTTTAAAAGGAAAGAATCAAAGTATGAGGGGAAATGGCAGAAAACAGTATCA; AGATTCACCTAATCAAAAGAAAAGAACAAATGGGCTTCAGCCAGCAAAGCAGCAGAATTCTTTGATGAAG; TGTGAAAAGAAACTTCATCCACGGAAACTTCAGGATAATTTTGAAACAGATGCTGTATATGACTTGCCTA; GCTCCAGTGAAGACCAGTTGCTAGAACATGCTGAGGGACAGCCCGTGGCATGTAATGGACATTGCAAGTT; CCCCTTTTCATCCAGAGCCTTTTTGAGTTTCAAGTTTGACCATAATGCTATAATGAAAATCTTGGACCTT; TGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_152624 unedited; GGGTGTCAACTTTTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGTCAGTC; CCCGGCCGCGCGGAGCCGGGATGCACTGTTCCTGCTGTGGGTCCTCATCATGGAGACCAA; ACGGGTGGAGATTCCCGGCAGCGTCCTGGACGATCTCTGCAGCCGATTTATTTTGCATAT; TCCCAGCGAGGAAAGAGACAATGCAATCCGAGTGTGTTTTCAGATTGAACTTGCCCATTG; GTTTTACTTGGATTTCTACATGCAGAACACACCAGGATTACCTCAGTGTGGGATAAGAGA; CTTTGCTAAAGCTGTCTTCAGTCATTGTCCGTTTTTGCTGCCTCAAGGTGAAGATGTGGA; AAAAGTTTTGGATGAATGGAAGGAATATAAAATGGGAGTACCAACATATGGTGCAATTAT; TCTTGATGAGACACTTGAAAATGTACTACTGGTTCAGGGGTACCTAGCAAAATCAGGCTG; GGGATTTCCAAAAGGAAAAGTAAATAAAGAAGAAGCTCCTCATGATTGTGCTGCTAGAGA; GGTCTTTGAAGAAACTGGTTTTGATATCAAAGACTATATTTGTAAGGATGATTACATTGA; ACTTCGAATCAATGACCAGCTTTGCTCGTTTGTACATCATTCCAGGAATTCCAAAAGACA; CAAAATTTAACCCAAAAACTAGAAGAGAAATTCGGAACATTGAGTGGTTCTCTATTGAGA; AATTGCCTTGTCATAGAAATGATATGACCCCCAAATCCAAACTTGGTTTGGCACCTAACA; AATTTTTTATGGGCCATTCCCTTTATCAGACCCATTAAGGACTGGCTTTCTCGAAGATTG; GCGATTCCTCAGAAGTGACAATGGATTTNCTCACTGGTAGCACGCCGCTAAACCACTGTG; GAANAATGAGTCGACCAAATTCGCCC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_152624 unedited; GTTACAGCTATGNNACCGCGGCCGCAATCTAGNGATCGGTTTTTTTTTTTTTTTTTTGGT; AGTGAAATGTTTTTAATGCACACATGCACTCAGACCTTAACTTTTCTATAGGATACAATA; TGGTGACTTTTATAGAAGAATCTAATTCCTATAGTAGGAGGCTGTTATTTACTCAGATTT; GAATTCCTTTTATTTGACATTACATACAAATTACTAAAGCCTTAATAAGGAAAAGTACCA; ATTCAATCTTACAGCATAAAATTACTTTCATTATATGTCTTCATTAGCTATACTTACTAA; TTGATATTTAATAGAAAAATAACGATATTCAAAGCTCAGAACAGCAAGTAGTAACCACAA; TTCCTTTGACAGCACTGGGAGACTTTCCTTTATACATGAATACAAAAATCCAACTTCATT; TTCCAACATCTTTAATCAAATTTCACTCACACTATATGAGTTCTATTAGGTTCTCAGGCC; TCTCCAAATGCTCATGTCTCAGGAGGGTATCACTATTTGAAATCCTAAACCATTCATTTG; CTACTTCTTAACAAAAATTTTTGAAAAGCATGTAAACCACACCCTTATCTTTTGGATTAC; AATGGTTAGGTACAAAAACAATTCATTTAGCATCTTATAAATCATTAAAGGTATACATAA; AAGCACCTACAAATTTTTTTAAAAATAAAAGATTCACAGTGCACTGGCACTTGGAACATA; TNAAGTTGGAACCGTACATTTAAAAAGTGAATTCCAAGACCTGTATCTCTCTCAAGACCT; TAAACCTTATGAAGCAAACCAATTCCTTTTCTAATTTACATCTTAAAATGCGCCCTCCCT; AAAAGTTAATGATTGGCATTTCGCCAAACCCCTTTAAACCTGGGTTCCCAAACCACACCA; AT;
• Restriction Sites: NotI-NotI
• ACCN: NM_152624
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_152624.4, NP_689837.2
• RefSeq Size: 10198 bp
• RefSeq ORF: 1263 bp
• Locus ID: 167227
• UniProt ID: Q8IU60
• Cytogenetics: 5q22.2
• Domains: DCP2, NUDIX
• Protein Pathways: RNA degradation
• Summary: The protein encoded by this gene is a key component of an mRNA-decapping complex required for degradation of mRNAs, both in normal mRNA turnover, and in nonsense-mediated mRNA decay (NMD). It removes the 7-methyl guanine cap structure from mRNA, prior to its degradation from the 5' end. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Jun 2011]; Transcript Variant: This variant (1) represents the predominant transcript, and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.