EXOC7 (NM_015219) Human Untagged Clone

SKU SC100441

EXOC7 (untagged)-Human exocyst complex component 7 (EXOC7), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: EXOC7
• Synonyms: 2-5-3p; BLOM4; EX070; EXO70; Exo70p; EXOC1; NEDSEBA; YJL085W
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_015219 edited; ATGATTCCCCCACAGGAGGCATCCGCTCGACGGCGGGAGATTGAGGACAAGCTGAAGCAG; GAGGAGGAGACTCTGTCCTTCATCCGAGACAGCCTGGAGAAGAGCGACCAGCTCACTAAG; AACATGGTGTCTATCTTATCATCCTTTGAGAGCCGCCTTATGAAGCTGGAGAACTCCATC; ATCCCTGTGCACAAGCAGACGGAGAATCTGCAGCGGCTGCAGGAGAATGTTGAGAAGACG; CTGTCCTGCCTGGACCATGTCATCAGCTACTACCATGTGGCCAGTGACACTGAGAAGATC; ATCAGAGAGGGCCCCACAGGTAGGCTGGAAGAGTACCTGGGAAGCATGGCCAAGATTCAG; AAGGCAGTGGAGTATTTCCAGGACAACAGCCCAGACAGCCCGGAACTCAACAAAGTGAAA; CTGCTCTTTGAGCGCGGGAAGGAGGCCCTGGAGTCCGAATTTCGCAGCCTGATGACGCGG; CACAGTAAGGTCGTCTCGCCCGTGCTCATCTTGGATCTGATCAGTGGTGACGATGATCTG; GAGGCCCAGGAGGACGTGACCCTGGAGCACCTGCCCGAGAGCGTGCTCCAGGATGTCATT; CGCATCTCCCGCTGGCTGGTGGAATATGGCCGCAACCAAGATTTCATGAACGTCTACTAC; CAGATACGCTCCAGCCAGCTGGACCGCTCCATCAAAGGACTGAAGGAGCATTTCCATAAG; AGCAGTTCTTCCTCTGGGGTTCCCTACTCCCCTGCTATCCCCAACAAGAGGAAAGACACA; CCTACCAAGAAGCCAGTCAAGCGGCCAGGGAGAGATGACATGCTGGACGTGGAGACCGAT; GCCTACATCCACTGCGTCAGTGCCTTCGTCAAGCTGGCGCAGAGCGAGTACCAGCTGCTG; GCCGACATCATCCCCGAGCACCACCAGAAGAAGACCTTCGACTCCCTGATACAGGATGCC; CTGGATGGGCTGATGCTTGAAGGGGAGAACATCGTGTCTGCTGCCCGGAAGGCCATTGTG; CGACACGACTTCTCCACGGTGCTCACCGTCTTCCCCATCCTGCGACACCTCAAGCAGACC; AAGCCTGAGTTTGACCAGGTGCTCCAGGGCACGGCTGCCAGCACAAAGAACAAGCTGCCT; GGCCTCATCACATCCATGGAGACCATCGGTGCCAAAGCGCTGGAGGACTTCGCAGACAAC; ATCAAGAATGACCCGGACAAGGAGTACAACATGCCGAAGGACGGCACCGTACACGAGCTC; ACCAGCAATGCCATCCTCTTCCTGCAGCAGCTTTTGGACTTCCAGGAGACGGCAGGCGCC; ATGCTGGCCTCCCAAGAGACCAGCTCTTCGGCCACCAGCTACAGCTCTGAGTTCAGCAAG; CGGCTGCTAAGCACCTATATCTGTAAAGTGCTGGGCAACCTGCAGTTGAACTTGCTGAGC; AAGTCCAAGGTGTACGAGGACCCAGCTCTGAGCGCCATCTTCCTGCACAACAACTACAAT; TACATCCTCAAGTCCCTGGAGAAGTCTGAACTGATCCAGCTGGTGGCAGTGACACAGAAG; ACTGCTGAGCGCTCCTACCGGGAGCACATTGAGCAGCAGATCCAGACCTACCAGCGCAGC; TGGTTAAAGGTGACTGATTACATCGCAGAGAAGAATCTACCTGTGTTCCAGCCGGGAGTC; AAGCTCCGGGACAAGGAGCGGCAGATTATCAAGGAGCGTTTTAAGGGCTTCAATGATGGC; CTCGAAGAACTGTGCAAAATCCAGAAGGCCTGGGCTATTCCAGACACAGAGCAGAGGGAC; AGGATTCGCCAGGCCCAGAAGACCATTGTCAAGGAGACCTACGGGGCCTTTCTACAGAAG; TTTGGCAGCGTGCCCTTCACCAAGAACCCGGAGAAGTACATCAAGTACGGGGTGGAGCAG; GTGGGCGACATGATCGATCGCCTTTTCGACACCTCTGCCTGA;
• Restriction Sites: NotI-NotI
• ACCN: NM_015219
• Insert Size: 3500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_015219.2, NP_056034.2
• RefSeq Size: 4687 bp
• RefSeq ORF: 1962 bp
• Locus ID: 23265
• UniProt ID: Q9UPT5
• Cytogenetics: 17q25.1
• Domains: Exo70
• Protein Families: Druggable Genome
• Protein Pathways: Insulin signaling pathway
• Summary: The protein encoded by this gene is a component of the exocyst complex. The exocyst complex plays a critical role in vesicular trafficking and the secretory pathway by targeting post-Golgi vesicles to the plasma membrane. The encoded protein is required for assembly of the exocyst complex and docking of the complex to the plasma membrane. The encoded protein may also play a role in pre-mRNA splicing through interactions with pre-mRNA-processing factor 19. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 4. [provided by RefSeq, Nov 2011]; Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.