PMPCA (NM_015160) Human Untagged Clone

SKU SC100435

PMPCA (untagged)-Human peptidase (mitochondrial processing) alpha (PMPCA), nuclear gene encoding mitochondrial protein

Product Data

• Type: Human Untagged Clone
• Target Symbol: PMPCA
• Synonyms: Alpha-MPP; CLA1; CPD3; INPP5E; MAS2; P-55; SCAR2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC100435 sequence for NM_015160 edited (data generated by NextGen Sequencing); ATGGCGGCTGTGGTGCTGGCGGCGACGCGGTTGCTGCGGGGCTCGGGTTCTTGGGGCTGT; TCGCGGCTGAGGTTTGGACCTCCTGCGTACAGACGGTTTAGTAGTGGTGGTGCCTATCCC; AACATCCCCCTCTCTTCTCCCTTACCTGGAGTACCCAAGCCTGTTTTTGCTACAGTTGAT; GGACAGGAAAAGTTTGAAACCAAAGTAACCACATTGGATAATGGGCTTCGCGTGGCATCT; CAGAATAAGTTTGGACAGTTTTGTACAGTAGGAATTCTTATCAATTCAGGATCGAGATAT; GAAGCGAAATACCTTAGTGGAATTGCTCACTTTTTGGAAAAATTGGCATTTTCGTCTACT; GCTCGATTTGACAGCAAAGATGAAATTCTGCTTACGTTGGAAAAGCATGGGGGTATCTGT; TACTGCCAGACATCAAGAGACACCACCATGTATGCTGTGTCTGCTGATAGCAAAGGCTTG; GACACGGTGGTTGCCTTACTGGCTGATGTGGTTCTGCAGCCCCGGCTAACAGATGAAGAA; GTCGAGATGACGCGGATGGCGGTCCAGTTTGAGCTGGAGGACCTGAACCTGCGGCCTGAC; CCAGAGCCACTTCTCACCGAGATGATTCATGAAGCGGCTTACAGGGAGAACACAGTTGGC; CTCCACCGTTTCTGCCCCACAGAAAACGTAGCAAAGATCAATCGAGAGGTGCTGCATTCC; TACCTGAGGAACTACTACACTCCCGACCGCATGGTGCTGGCCGGCGTGGGCGTGGAGCAC; GAGCATCTGGTGGACTGTGCCCGGAAGTACCTCCTGGGGGTCCAGCCGGCCTGGGGGAGC; GCAGAGGCCGTGGATATTGACAGATCTGTGGCCCAGTACACTGGGGGGATTGCCAAGCTA; GAAAGAGACATGTCCAATGTCAGCCTGGGCCCGACCCCCATCCCCGAGCTCACGCACATC; ATGGTTGGACTGGAGAGCTGCTCCTTCCTGGAGGAGGACTTCATCCCCTTTGCAGTGTTG; AACATGATGATGGGCGGAGGTGGCTCCTTCTCGGCTGGTGGGCCCGGCAAGGGCATGTTC; TCCAGGCTCTACCTCAACGTGCTCAACAGGCACCACTGGATGTATAACGCGACCTCCTAC; CACCACAGCTACGAGGACACTGGCCTCCTTTGCATCCATGCCAGCGCCGACCCAAGACAG; GTTCGAGAAATGGTAGAAATCATCACAAAGGAGTTTATTTTAATGGGCGGAACCGTGGAC; ACGGTGGAGCTGGAACGAGCCAAGACGCAGCTGACATCAATGCTCATGATGAACCTGGAA; TCCAGGCCTGTGATCTTCGAGGATGTGGGGAGGCAGGTGCTGGCCACTCGCTCCAGAAAG; CTGCCGCACGAGCTGTGCACGCTCATCCGCAACGTGAAGCCGGAAGATGTGAAGAGAGTC; GCTTCTAAGATGCTCCGAGGGAAGCCGGCAGTGGCCGCCCTGGGTGACCTGACTGACCTG; CCCACGTATGAGCACATCCAGACCGCCCTGTCGAGTAAGGACGGGCGCCTGCCCAGGACG; TACCGGCTCTTCCGGTAG; ; Clone variation with respect to NM_015160.1; 421 g=>t;702 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015160 unedited; TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGCAAGATGGCGGCTG; TGGTGCTGGCGGCGACGCGGTTGCTGCGGGGCTCGGGTTCTTGGGGCTGTTCGCGGCTGA; GGTTTGGACCTCCTGCGTACAGACGGTTTAGTAGTGGTGGTGCCTATCCCAACATCCCCC; TCTCTTCTCCCTTACCTGGAGTACCCAAGCCTGTTTTTGCTACAGTTGATGGACAGGAAA; AGTTTGAAACCAAAGTAACCACATTGGATAATGGGCTTCGCGTGGCATCTCAGAATAAGT; TTGGACAGTTTTGTACAGTAGGAATTCTTATCAATTCAGGATCGAGATATGAAGCGAAAT; ACCTTAGTGGAATTGCTCACTTTTTGGAAAAATTGGCATTTTCGTCTACTGCTCGATTTG; ACAGCAAAGATGAAATTCTGCTTACGTTGGAAAAGCATGGGGGTATCTGTTACTGCCAGA; CATCAAGAGACACCACCATGTATGCTGTGTCTGCTGATAGCAAAGGCTTGGACACGGTGG; TTGCCTTACTGGCTGATGTGGTTCTGCAGCCCCGGCTAACAGATGAAGAAGTCGAGATGA; CGCGGATGGCGGTCCAGTTTGAGCTGGAGGACCTGAACCTGCGGCCTGACCCAGAGCCAC; TTCTCACCGAGATGATTCATGAAGCGGCTTACAGGGAGAACACAGTTGGCCTNCACCGTT; TCTGCCCCACAGAANACGTAGCANAGATCAATCGAGAGGTGCTGCATTCCTACCTNGAGA; ACTACTACACTCCCGACCGCATGGTGCTGNCCNGCGTGGGCGTGNAGACGAGCATCTGGT; GGACTGTGCCCGGAAGTCCCTNCTGGGGTCCAGCCNGNCTGNGGGACGCANAGCCGTGNT; ATTGACAGACTGTGCCCATACCTGGGGGGGATGCAGCTAGAGAGACTGTCATGTCAGCTG; GCCCGACCCATNCCGACTCGCCANATGGTGGACTGAACTGCTCCCTGAGAG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_015160 unedited; GTACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTAAGAAAGATGCCGC; TCTTTATTACCAGGGAAGCTGTGTGCACGCGCGTGGAGCGCGCTCCTTGGAGCTGACCGG; GCCCTTACCTTCTCCTGCTTGTCAGAGGTGAGTCCTGGTACCCAGCACGGTGGCCTCTGG; GAGGCTCTGATAGGTCAGCCTTTGCTGCCTCCCAGCTCAGGGCTCCTCCAAGGAACCTGC; GGGGCCCCATGTGCCCACAGCCCGAGGAGGGAAGCACCGACCGCCCTCCTCGTGGCACGC; TGCACTCCAGCGCTGGGCACTTCACCTGCTTCCTGGCTTGCGGTGGTCAGGCTCGATACT; GACTCCACTGATCGGCACATTGAGTTCCGAAAGAATGCAAACCGCGTGGGTGCTGTGGCG; ACATTGTTTGCACCGTTTATACAACCAGACAGCTTTTTAGACTAAATTCGTGTCCAAACT; AACACGCACGGGAACGGGCTCCAGCTGCAGCTCCCTGGGTCTGTCAGGCCGGGGAGCGGT; TCTACCGGAAGAGCCGGTACGTCCTGGGCAGGCGCCCGTCCTTACTCGACAGGGCGGTCT; GGATGTGCTCATACGTGGGCAGGTCAGTCAGGTCACCCAGGGGCGGCCACTGCCGGCTTC; CCTCGGAGCATCTTAGAAGCGACTCTCTTCACATCTTCCGGGCTCACGTTTGCGATGAGC; GTGCACAGCTCGTGCGGGAGCTTTCTGGAGCGAGTGGCCAGCACCTGCCTCCCCAATCCT; CGAAGATAACAGGCCTGGATTCAGGTTCATCATGAGCATTGATGTCAGCTGCGTCTGGCC; TCGTTCCAGCTTACCCGGGTCCCGGGTCCGCCCATAAAATAAACCCCTTGGGAAGAATCT; TACCATTTTCCAACCTGCTTTGGTCCGCCCTGGCCTGATACCAAGAAGCCCTGTCCTCTT; ACTTGGTGGTAGGAGGCCCCGTTATCATCCGGGGGG;
• Restriction Sites: NotI-NotI
• ACCN: NM_015160
• Insert Size: 2300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_015160.1, NP_055975.1
• RefSeq Size: 2097 bp
• RefSeq ORF: 1578 bp
• Locus ID: 23203
• UniProt ID: Q10713
• Cytogenetics: 9q34.3
• Domains: Peptidase_M16, Peptidase_M16_C
• Protein Families: Druggable Genome, Protease
• Summary: The protein encoded by this gene is found in the mitochondrion, where it represents the alpha subunit of a proteolytic heterodimer. This heterodimer is responsible for cleaving the transit peptide from nuclear-encoded mitochondrial proteins. Defects in this gene are a cause of spinocerebellar ataxia, autosomal recessive 2. [provided by RefSeq, Mar 2016]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.