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IFIT2 (NM_001547) Human Untagged Clone

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SKU
SC100402
IFIT2 (untagged)-Human interferon-induced protein with tetratricopeptide repeats 2 (IFIT2)
  $457.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol IFIT2
Synonyms cig42; G10P2; GARG-39; IFI-54; IFI-54K; IFI54; IFIT-2; ISG-54 K; ISG-54K; ISG54; P54
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC100402 sequence for NM_001547 edited (data generated by NextGen Sequencing)
ATGAGTGAGAACAATAAGAATTCCTTGGAGAGCAGCCTACGGCAACTAAAATGCCATTTC
ACCTGGAACTTGATGGAGGGAGAAAACTCCTTGGATGATTTTGAAGACAAAGTATTTTAC
CGGACTGAGTTTCAGAATCGTGAATTCAAAGCCACAATGTGCAACCTACTGGCCTATCTA
AAGCACCTCAAAGGGCAAAACGAGGCAGCCCTGGAATGCTTACGTAAAGCTGAAGAGTTA
ATCCAGCAAGAGCATGCTGACCAGGCAGAAATCAGAAGTCTGGTCACCTGGGGAAACTAT
GCCTGGGTCTACTATCACATGGGCCGACTCTCAGACGTTCAGATTTATGTAGACAAGGTG
AAACATGTCTGTGAGAAGTTTTCCAGTCCCTATAGAATTGAGAGTCCAGAGCTTGACTGT
GAGGAAGGGTGGACACGGTTAAAGTGTGGAGGAAACCAAAATGAAAGAGCGAAGGTGTGC
TTTGAGAAGGCTCTGGAAAAGAAGCCAAAGAACCCAGAATTCACCTCTGGACTGGCAATA
GCAAGCTACCGTCTGGACAACTGGCCACCATCTCAGAACGCCATTGACCCTCTGAGGCAA
GCCATTCGGCTGAATCCTGACAACCAGTACCTTAAAGTCCTCCTGGCTCTGAAGCTTCAT
AAGATGCGTGAAGAAGGTGAAGAGGAAGGTGAAGGAGAGAAGTTAGTTGAAGAAGCCTTG
GAGAAAGCCCCAGGTGTAACAGATGTTCTTCGCAGTGCAGCCAAGTTTTATCGAAGAAAA
GATGAGCCAGACAAAGCGATTGAACTGCTTAAAAAGGCTTTAGAATACATACCAAACAAT
GCCTACCTGCATTGCCAAATTGGGTGCTGCTATAGGGCAAAAGTCTTCCAAGTAATGAAT
CTAAGAGAGAATGGAATGTATGGGAAAAGAAAGTTACTGGAACTAATAGGACACGCTGTG
GCTCATCTGAAGAAAGCTGATGAGGCCAATGATAATCTCTTCCGTGTCTGTTCCATTCTT
GCCAGCCTCCATGCTCTAGCAGATCAGTATGAAGACGCAGAGTATTACTTCCAAAAGGAA
TTCAGTAAAGAGCTTACTCCTGTAGCGAAACAACTGCTCCATCTGCGGTATGGCAACTTT
CAGCTGTACCAAATGAAGTGTGAAGACAAGGCCATCCACCACTTTATAGAGGGTGTAAAA
ATAAACCAGAAATCAAGGGAGAAAGAAAAGATGAAAGACAAACTGCAAAAAATTGCCAAA
ATGCGACTTTCTAAAAATGGAGCAGATTCTGAGGCTTTGCATGTCTTGGCATTCCTTCAG
GAGCTGAATGAAAGAATGCAACAAGCAGATGAAGACTCTGAGAGGGGTTTGGAGTCTGGA
AGCCTCATCCCTTCAGCATCAAGCTGGAATGGGGAATGA

Clone variation with respect to NM_001547.4
1334 a=>g
5' Read Nucleotide Sequence
>OriGene 5' read for NM_001547 unedited
TTCAAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAAATTCGCACGAGGAGAAGAG
GAAGATTTCTGAAGAGTGCAGCTGCCTGAACCGAGCCCTGCCGAACAGCTGAGAATTGCA
CTGCAACCATGAGTGAGAACAATAAGAATTCCTTGGAGAGCAGCCTACGGCAACTAAAAT
GCCATTTCACCTGGAACTTGATGGAGGGAGAAAACTCCTTGGATGATTTTGAAGACAAAG
TATTTTACCGGACTGAGTTTCAGAATCGTGAATTCAAAGCCACAATGTGCAACCTACTGG
CCTATCTAAAGCACCTCAAAGGGCAAAACGAGGCAGCCCTGGAATGCTTACGTAAAGCTG
AAGAGTTAATCCAGCAAGAGCATGCTGACCAGGCAGAAATCAGAAGTCTGGTCACCTGGG
GAAACTATGCCTGGGTCTACTATCACATGGGCCGACTCTCAGACGTTCAGATTTATGTAG
ACAAGGTGAAACATGTCTGTGAGAAGTTTTCCAGTCCCTATAGAATTGAGAGTCCAGAGC
TTGACTGTGAGGAAGGGTGGACACGGTTAAAGTGTGGAGGAAACCAAAATGAAAGAGCGA
AGGTGTGCTTTGAGAAGGCTCTGGAAAAGAAGCCAAAGAACCCAGAATTCACCTCTGGAC
TGGCAATAGCAAGCTACCGTCTGGACAACTGGCCACCATCTCAGAACGCCATTGACCCTC
TGAGGCAGCCATTCGGCTGAATCCTGANCACCAGTACCTTAAAGTCCTNCTGGCTCTGAA
GCTTCATAAATGCGTGAANAANGTGAANAGGAANGTGAAGAGAGAAGTTAGTGAAGAAGC
CTTGGAGAAGCCCCAGTGTAACAGAATGTCTTCGCATGCAGCCCAGTTTATTCGAGAAAA
GAGAGCCAGACAAAGCGATGACTGCTTAAAAGGCTTAGATACATACCAACATGCCTCC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_001547 unedited
NTTTGGTATCATCTATGNNACCGCGGCCGCATNCTAGNGATCGATTTTTTTTTTTTTTTT
TTTATGTTTTATCATATTTAGGTTATTTATTAATGAAAATATATGACATTTTCAGGAATA
CAAATTTTGCACCCTGATGACCTCAAATGCGTGCAACAAGATGTTTAATACAGAAAATAA
CACAAAAACTGTTGTTACAGTGGTTAGAATTTTTAACTTTAAAAAACCATGAATTTGTAT
TGTTTTAATTGCACAATAAAATAATGTTGATATATACTTAAGCTTAAATTAATTCCAACA
GGCAAACATTTTCCAACCCAGAGTGTGGCTGATGCTGGGTCAGCTGACTCTATTTTGGTT
CACACCAACTTTGATGTCTAGGCTATTCAGCATCTACCTAGAAAATCTCAATCGTTCCAA
GCATACCGTGAATTTTGTGATTTCTCAGAAGATTTTCGGAGTTAAAAGAAGTGTTTATAT
CACTTAATATCCAACATTTCTAAAGGGGGGAAAACCCCACCATCTATTATCAATGACATT
TCCCAAGTCCTTGCACCAGGCCCTTAGTCACCAGGTTCCCACGTTTTGTTGCTTTCCTAC
CGTCTCAAACCAGGTTCATGAAAGCATTTGAACAGAGTTCAGTCTTCATTTTACAAAAAA
AAAAAAATTCCTAATAGTGGCTAAAATACTGCAAATTTCTCCATTCGATTACAATCTACA
AAGATAAACCAGCATTCCTTTCTCTTTCTCTCTTTTGGTTACTTTTCCCCTATCCCTACT
GGTGAGACATTTATTTTTTCACACAGTTTTTAAAGAATACATTATGTTGGGTTCTAGTGT
ATTGTACAGAATCCATTCTCATCCTTTACTTGCTACATTATGACTATGAGGA
Restriction Sites NotI-NotI |
ACCN NM_001547
Insert Size 3500 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001547.3, NP_001538.3
RefSeq Size 3058 bp
RefSeq ORF 1455 bp
Locus ID 3433
UniProt ID P09913
Cytogenetics 10q23.31
Domains TPR
Summary IFN-induced antiviral protein which inhibits expression of viral messenger RNAs lacking 2'-O-methylation of the 5' cap. The ribose 2'-O-methylation would provide a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Viruses evolved several ways to evade this restriction system such as encoding their own 2'-O-methylase for their mRNAs or by stealing host cap containing the 2'-O-methylation (cap snatching mechanism). Binds AU-rich viral RNAs, with or without 5' triphosphorylation, RNA-binding is required for antiviral activity. Can promote apoptosis.UniProtKB/Swiss-Prot Function
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"NM_001547" in other vectors (6)
SKU Description Size Price
RC205582 IFIT2 (Myc-DDK-tagged)-Human interferon-induced protein with tetratricopeptide repeats 2 (IFIT2) 10 ug
$457.00
RC205582L1 Lenti ORF clone of Human interferon-induced protein with tetratricopeptide repeats 2 (IFIT2), Myc-DDK-tagged 10 ug
$757.00
RC205582L2 Lenti ORF clone of Human interferon-induced protein with tetratricopeptide repeats 2 (IFIT2), mGFP tagged 10 ug
$757.00
RC205582L3 Lenti ORF clone of Human interferon-induced protein with tetratricopeptide repeats 2 (IFIT2), Myc-DDK-tagged 10 ug
$757.00
RC205582L4 Lenti ORF clone of Human interferon-induced protein with tetratricopeptide repeats 2 (IFIT2), mGFP tagged 10 ug
$757.00
RG205582 IFIT2 (tGFP-tagged) - Human interferon-induced protein with tetratricopeptide repeats 2 (IFIT2) 10 ug
$489.00 $657.00

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