IL33 (NM_033439) Human Untagged Clone
$450.00
5 Days*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | IL33 |
| Synonyms | C9orf26; DVS27; IL1F11; NF-HEV; NFEHEV |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites | NotI-NotI | |
| ACCN | NM_033439 |
| Insert Size | 2630 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_033439.2, NP_254274.1 |
| RefSeq Size | 2644 bp |
| RefSeq ORF | 813 bp |
| Locus ID | 90865 |
| UniProt ID | O95760 |
| Cytogenetics | 9p24.1 |
| Protein Families | Secreted Protein |
| Protein Pathways | Cytosolic DNA-sensing pathway |
| Summary | The protein encoded by this gene is a cytokine that binds to the IL1RL1/ST2 receptor. The encoded protein is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. Several transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Sep 2015 Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a, also known as 1). Variants 1, 4, and 5 all encode the same isoform (a). |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_033439" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC207989 | IL33 (Myc-DDK-tagged)-Human interleukin 33 (IL33), transcript variant 1 | 10 ug |
$450.00
|
|
| RC207989L1 | Lenti ORF clone of Human interleukin 33 (IL33), transcript variant 1, Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC207989L2 | Lenti ORF clone of Human interleukin 33 (IL33), transcript variant 1, mGFP tagged | 10 ug |
$750.00
|
|
| RC207989L3 | Lenti ORF clone of Human interleukin 33 (IL33), transcript variant 1, Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC207989L4 | Lenti ORF clone of Human interleukin 33 (IL33), transcript variant 1, mGFP tagged | 10 ug |
$750.00
|
|
| RG207989 | IL33 (tGFP-tagged) - Human interleukin 33 (IL33), transcript variant 1 | 10 ug |
$650.00
|
|
Citations
Glioma-derived IL-33 orchestrates an inflammatory brain tumor microenvironment that accelerates glioma progression
De Boeck, Ahn, D'Mello et al
Nat Commun (2020) 11 (1), 4997 DOI: 10.1038/s41467-020-18569-4
De Boeck, Ahn, D'Mello et al
Nat Commun (2020) 11 (1), 4997 DOI: 10.1038/s41467-020-18569-4
Cited by 185
AI Summary: In this study, OriGene products including the IL-33 cDNA clone and IL-33 shRNA lentiviral particles were used to manipulate IL-33 expression in glioma cells, enabling the authors to investigate how IL-33 influences the tumor microenvironment and glioma progression.
Snippet: Despite a deeper molecular understanding, human glioblastoma remains one of the most treatment refractory and fatal cancers. It is known that the presence of macrophages and microglia impact glioblastoma tumorigenesis and prevent durable response. Herein we
IL-33 promotes growth and liver metastasis of colorectal cancer in mice by remodeling the tumor microenvironment and inducing angiogenesis
Zhang, Davis, Shah et al
Mol. Carcinog. (2016)
Zhang, Davis, Shah et al
Mol. Carcinog. (2016)
Cited by 154
AI Summary: OriGene’s mouse and human Il33 cDNAs were used to generate plasmids for transgenic expression of IL-33 in colorectal cancer cell lines, enabling the study of IL-33’s role in tumor growth and liver metastasis in this paper.
A functional DC cross talk promotes human ILC homeostasis in humanized mice
Lopez-Lastra, Masse-Ranson, Fiquet et al
Blood Advances (2017) 1, 601
Lopez-Lastra, Masse-Ranson, Fiquet et al
Blood Advances (2017) 1, 601
Snippet: Hydrodynamic injection Full-length human IL-2, IL-7, IL-25, and IL-33 complementary DNA clones were cloned in the mammalian cell expression vector pCMV- 6-XL4 (Origene) and plasmids purified using the endotoxin-free Plasmid-Maxi kit (Qiagen);;Full-length human IL-2, IL-7, IL-25, and IL-33 complementary DNA clones were cloned in the mammalian cell expression vector pCMV-6-XL4 (Origene) and plasmids...
Showing 1-3 of 3 papers.
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complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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