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Chrna4 (NM_024354) Rat Untagged Clone

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SKU
RN211661
Chrna4 (untagged ORF) - Rat cholinergic receptor, nicotinic, alpha 4 (Chrna4), (10 ug)
  $587.00
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Specifications
Specifications
Product Data
Type Rat Untagged Clone
Target Symbol Chrna4
Synonyms NARAC
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>RN211661 representing NM_024354
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCAATTCGGGCCCCGGGGCGCCGCCGCCGCTGCTGCTACTGCCGCTGCTGCTGCTCCTAGGGACCG
GCCTCTTGCCTGCTAGCAGCCACATAGAGACCCGGGCCCATGCGGAGGAGCGGCTCCTGAAGAGACTCTT
CTCCGGTTACAACAAGTGGTCTCGGCCAGTAGGCAATATCTCAGATGTGGTCCTCGTCCGCTTTGGCTTG
TCCATTGCTCAGCTCATTGACGTGGACGAGAAGAACCAGATGATGACAACCAACGTGTGGGTGAAGCAGG
AGTGGCACGACTACAAGCTGCGCTGGGACCCTGGTGACTACGAGAATGTCACCTCCATCCGCATCCCCTC
TGAACTCATCTGGAGGCCTGACATCGTCCTCTACAACAATGCGGATGGAGACTTTGCAGTCACCCACCTG
ACCAAGGCCCACCTGTTCTATGACGGAAGGGTGCAGTGGACACCCCCAGCCATCTATAAGAGCTCCTGCA
GCATCGACGTCACCTTCTTCCCCTTTGACCAGCAGAACTGTACCATGAAGTTTGGATCCTGGACCTACGA
CAAGGCCAAGATTGACTTAGTGAGCATTCATAGCCGTGTGGACCAACTGGACTTCTGGGAAAGTGGGGAG
TGGGTCATCGTGGATGCTGTGGGCACCTACAACACCAGGAAGTACGAGTGCTGTGCCGAGATCTATCCTG
ACATCACCTATGCCTTCATCATCCGACGCCTGCCGCTATTCTACACCATCAACCTCATCATCCCGTGCCT
GCTCATCTCCTGTCTCACCGTGCTGGTCTTCTATCTGCCTTCAGAGTGTGGCGAGAAGGTCACACTGTGC
ATCTCGGTGCTGCTTTCTCTCACCGTCTTCCTGCTGCTCATCACCGAGATCATCCCGTCCACCTCGCTGG
TCATCCCGCTCATCGGCGAGTACCTCCTCTTCACCATGATCTTCGTCACCCTCTCCATCGTCATCACGGT
CTTCGTGCTCAATGTGCACCACCGCTCGCCACGCACACACACGATGCCCGCCTGGGTGCGTAGAGTCTTC
CTGGACATCGTGCCTCGCCTCCTCTTCATGAAGCGCCCCTCTGTGGTCAAAGACAACTGCCGGAGACTTA
TTGAGTCCATGCACAAGATGGCCAACGCCCCCCGCTTCTGGCCAGAGCCTGTGGGCGAGCCCGGCATCTT
GAGTGACATCTGCAACCAAGGTCTGTCACCTGCCCCAACTTTCTGCAACCCCACGGACACAGCAGTCGAG
ACCCAGCCTACGTGCAGGTCACCCCCCCTTGAGGTCCCTGACTTGAAGACATCAGAGGTTGAGAAGGCCA
GTCCCTGTCCATCGCCTGGCTCCTGTCCTCCACCCAAGAGCAGCAGTGGGGCTCCAATGCTCATCAAAGC
CAGGTCCCTGAGTGTCCAGCATGTGCCCAGCTCCCAAGAAGCAGCAGAAGATGGCATCCGCTGCCGGTCT
CGGAGTATCCAGTACTGTGTTTCCCAAGATGGAGCTGCCTCCCTGGCTGACAGCAAGCCCACCAGCTCCC
CGACCTCCCTGAAGGCCCGTCCATCCCAGCTTCCCGTGTCAGACCAGGCCTCTCCATGCAAATGCACATG
CAAGGAACCATCTCCTGTGTCCCCAGTCACTGTGCTCAAGGCGGGAGGCACCAAAGCACCTCCCCAACAC
CTGCCCCTGTCACCAGCCCTGACACGGGCAGTAGAAGGCGTCCAGTACATTGCAGACCACCTCAAGGCAG
AAGACACTGACTTCTCGGTGAAGGAGGACTGGAAATACGTGGCCATGGTCATTGACCGAATCTTCCTCTG
GATGTTCATCATTGTCTGCCTTCTGGGCACTGTGGGACTCTTCCTGCCTCCCTGGCTGGCTGGTATGATC
TAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI |
ACCN NM_024354
Insert Size 1893 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024354.1, NP_077330.1
RefSeq Size 2149 bp
RefSeq ORF 1893 bp
Locus ID 25590
UniProt ID P09483
Cytogenetics 3q43
Summary nicotinic acetylcholine receptor subunit responsible for binding calcium sensor protein visinin-like protein-1 (Vilip1), which modulates acetylcholine receptor expression and sensitivity RGD, Feb 2006
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"NM_024354" in other vectors (3)
SKU Description Size Price
RR211661 Chrna4 (Myc-DDK-tagged ORF) - Rat cholinergic receptor, nicotinic, alpha 4 (Chrna4), (10 ug) 10 ug
$645.00
RR211661L3 Lenti ORF clone of Chrna4 (Myc-DDK-tagged ORF) - Rat cholinergic receptor, nicotinic, alpha 4 (Chrna4), (10 ug) 10 ug
$945.00
RR211661L4 Lenti ORF clone of Chrna4 (mGFP-tagged ORF) - Rat cholinergic receptor, nicotinic, alpha 4 (Chrna4), (10 ug) 10 ug
$945.00

file-alt Citations

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