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Tnfsf11 (NM_057149) Rat Untagged Clone

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SKU
RN211558
Tnfsf11 (untagged ORF) - Rat tumor necrosis factor (ligand) superfamily, member 11 (Tnfsf11), (10 ug)
  $330.00
5 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Rat Untagged Clone
Target Symbol Tnfsf11
Synonyms RANKL
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>RN211558 representing NM_057149
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGCCGGGCCAACCGAGACTACGGCAAGTACCTGCGCGGCTCGGAGGAGATGGGCAGTTGCCCTGGCG
TCCCACACGAGGGTCCGCTGCATCCCGCGCCTTCAGCACCGGCTCCAGCGCCGCCCCCCGCCGCCTCCCG
CTTCATGTTCCTGGCGCTCCTGGGGCTGGGACTGGGTCAGGTGGTCTGCAGCATCGCTCTGTTCCTGTAC
TTTCGAGCGCAGATGGATCCTAACAGAATATCAGAAGACAGCACGCGCTGCTTCTACAGAATTCTGAGAC
TCCGTGAAAATACAGGTTTGCAGGACTCGACTCTGGAGAGCGAAGACACAGAAGCACTACCTGACTCATG
CAGGAGAATGAAACAAGCCTTTCAAGGGGCCGTGCAAAGGGAATTACAACACATTGTGGGGCCACAGCGC
TTCTCAGGAGTTCCAGCTATGATGGAAGGTTCGTGGCTCGATGTGGCCCGGCGGGGCAAGCCTGAGGCTC
AGCCGTTTGCTCACCTCACCATCAATGCTGCCGACATCCCATCGGGTTCCCATAAAGTCAGTCTGTCCTC
TTGGTACCATGATCGAGGCTGGGCCAAGATCTCTAACATGACGTTAAGCAACGGAAAACTAAGGGTTAAC
CAAGATGGCTTCTATTACCTGTACGCCAACATTTGCTTCAGGCATCATGAAACCTCAGGGAGCGTACCTG
CGGACTATCTTCAGCTGATGGTATATGTCGTTAAAACCAGCATCAAAATCCCAAGTTCGCATAACCTGAT
GAAAGGGGGGAGCACTAAGAACTGGTCAGGGAATTCTGAATTCCACTTTTATTCCATAAACGTTGGAGGA
TTTTTCAAGCTCCGGGCTGGTGAGGAAATTAGCGTCCAGGTGTCCAACCCTTCCCTGTTGGATCCGGATC
AAGATGCGACGTACTTTGGGGCTTTCAAAGTTCAAGACATAGACTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_057149
Insert Size 957 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_057149.1, NP_476490.1
RefSeq Size 957 bp
RefSeq ORF 957 bp
Locus ID 117516
UniProt ID Q9ESE2
Cytogenetics 15q11
Summary induces osteoclast formation and bone resoprtion; plays a role in regulation of calcium homeostasis and osteoclast function RGD, Feb 2006
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Documents
Resources
"NM_057149" in other vectors (3)
SKU Description Size Price
RR211558 Tnfsf11 (Myc-DDK-tagged ORF) - Rat tumor necrosis factor (ligand) superfamily, member 11 (Tnfsf11), (10 ug) 10 ug
$289.00 $330.00
RR211558L3 Lenti ORF clone of Tnfsf11 (Myc-DDK-tagged ORF) - Rat tumor necrosis factor (ligand) superfamily, member 11 (Tnfsf11), (10 ug) 10 ug
$630.00
RR211558L4 Lenti ORF clone of Tnfsf11 (mGFP-tagged ORF) - Rat tumor necrosis factor (ligand) superfamily, member 11 (Tnfsf11), (10 ug) 10 ug
$630.00

file-alt Citations

LOX Fails to Substitute for RANKL in Osteoclastogenesis
Tsukasaki, Hamada, Okamoto et al
J. Bone Miner. Res. (2016)
Cited by 54
AI Summary: OriGene’s recombinant LOX (rLOX) protein was used in this study to assess whether LOX could induce osteoclastogenesis or substitute for RANKL, demonstrating that rLOX from OriGene failed to replace RANKL in promoting osteoclast differentiation.
Snippet: Reagents The following reagents were used: rLOX (OriGene Technologies) Lot # 06J09L15 / rLOX (Mouse) (Cloud-Clone Corp) Lot # P20150715497 / Amplite ... In vivo injection of LOX and RANKL Recombinant LOX (OriGene Technologies) in saline (50 μg kg ... Cite SaveSaving
Showing 1-1 of 1 papers.
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