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Prkaa2 (NM_023991) Rat Untagged Clone

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SKU
RN208871
Prkaa2 (untagged ORF) - Rat protein kinase, AMP-activated, alpha 2 catalytic subunit (Prkaa2), (10 ug)
  $772.00
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Specifications
Specifications
Product Data
Type Rat Untagged Clone
Target Symbol Prkaa2
Synonyms Ampk; Ampka2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>RN208871 representing NM_023991
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGAGAAGCAGAAGCACGACGGGCGTGTGAAGATCGGACACTACGTGCTGGGGGACACCCTGGGCG
TCGGCACCTTCGGCAAAGTGAAGATTGGAGAACATCAATTGACAGGCCATAAAGTGGCAGTTAAGATCTT
AAATAGACAGAAGATTCGCAGTTTAGATGTTGTTGGAAAAATAAAACGAGAAATTCAAAATCTTAAACTC
TTTCGTCATCCTCATATTATCAAACTCTACCAAGTGATCAGCACTCCAACAGACTTTTTTATGGTAATGG
AATATGTGTCTGGAGGTGAATTGTTCGACTACATCTGTAAACACGGGAGGGTTGAAGAGGTGGAAGCTCG
CCGGCTCTTCCAGCAGATTCTGTCTGCCGTGGACTACTGTCACAGGCACATGGTTGTCCACAGGGACCTG
AAGCCAGAGAACGTGTTGCTGGACGCCCAGATGAATGCTAAGATAGCTGACTTCGGACTCTCTAATATGA
TGTCAGATGGTGAATTTCTACGAACTAGCTGTGGATCGCCAAATTATGCAGCACCGGAGGTCATCTCAGG
AAGGCTGTATGCGGGTCCTGAGGTTGATATCTGGAGCTGTGGTGTTATCCTGTATGCCCTTCTCTGTGGC
ACCCTCCCGTTCGACGATGAGCACGTGCCTACGCTCTTTAAGAAGATCCGAGGGGGTGTGTTCTACATCC
CGGAGTATCTCAACCGTTCTATTGCCACTCTGCTGATGCACATGCTGCAGGTGGACCCCTTGAAGCGAGC
AACTATCAAAGACATACGAGAGCATGAATGGTTTAAACAGGATTTGCCCAGTTACCTCTTTCCTGAAGAC
CCCTCCTATGATGCTAACGTCATTGATGATGAGGCTGTGAAAGAAGTATGTGAAAAATTTGAGTGTACAG
AATCAGAAGTGATGAACAGTTTATACAGTGGTGACCCTCAAGACCAGCTCGCAGTGGCTTATCATCTCAT
CATTGACAATCGGAGAATAATGAACCAAGCCAGTGAGTTCTACCTCGCCTCCAGTCCTCCAACGGGTTCC
TTCATGGACGATATGGCCATGCACATTCCCCCCGGCCTGAAACCACATCCTGAAAGGATGCCACCTCTCA
TAGCAGACAGCCCCAAAGCACGCTGTCCACTGGATGCACTCAACACAACTAAGCCCAAATCTTTAGCTGT
GAAAAAAGCCAAGTGGCACCTTGGGATCCGAAGCCAGAGCAAACCATACGACATTATGGCGGAGGTGTAC
CGAGCTATGAAGCAGCTGGACTTTGAATGGAAGGTAGTGAATGCATACCATCTTCGAGTAAGAAGAAAAA
ACCCAGTGACTGGCAATTACGTGAAAATGAGCTTACAGCTTTACCTGGTTGACAATCGGAGCTATCTTCT
AGACTTTAAAAGCATCGATGATGAGGTGGTGGAGCAGAGGTCTGGTTCTTCAACACCTCAGCGCTCCTGT
TCTGCTGCCGGCCTCCACAGACCTCGGTCAAGTGTCGATTCCAGCACAGCCGAGAACCATTCACTGTCTG
GCTCTCTCACTGGTTCTTTGACTGGCAGCACTTTGTCCTCCGCTTCCCCGCGCCTGGGCAGTCATACCAT
GGATTTTTTTGAAATGTGCGCCAGTCTTATCACTGCTTTAGCCCGTTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI |
ACCN NM_023991
Insert Size 1659 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_023991.1, NP_076481.1
RefSeq Size 2652 bp
RefSeq ORF 1659 bp
Locus ID 78975
UniProt ID Q09137
Cytogenetics 5q34
Summary protein kinase that phosphorylates and inhibits acetyl-CoA carboxylase (ACC); rate-limiting enzyme in malonyl-CoA synthesis RGD, Feb 2006
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Documents
Resources
"NM_023991" in other vectors (5)
SKU Description Size Price
RR208871 Prkaa2 (Myc-DDK-tagged ORF) - Rat protein kinase, AMP-activated, alpha 2 catalytic subunit (Prkaa2), (10 ug) 10 ug
$822.00
RR208871L1 Lenti ORF clone of Prkaa2 (Myc-DDK-tagged ORF) - Rat protein kinase, AMP-activated, alpha 2 catalytic subunit (Prkaa2), (10 ug) 10 ug
$1,122.00
RR208871L2 Lenti ORF clone of Prkaa2 (mGFP-tagged ORF) - Rat protein kinase, AMP-activated, alpha 2 catalytic subunit (Prkaa2), (10 ug) 10 ug
$1,122.00
RR208871L3 Lenti ORF clone of Prkaa2 (Myc-DDK-tagged ORF) - Rat protein kinase, AMP-activated, alpha 2 catalytic subunit (Prkaa2), (10 ug) 10 ug
$1,122.00
RR208871L4 Lenti ORF clone of Prkaa2 (mGFP-tagged ORF) - Rat protein kinase, AMP-activated, alpha 2 catalytic subunit (Prkaa2), (10 ug) 10 ug
$1,122.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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