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Wls (NM_199408) Rat Untagged Clone

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SKU
RN200060
Wls (untagged ORF) - Rat G protein-coupled receptor 177 (Gpr177), transcript variant 1, (10 ug)
  $807.00
5 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Rat Untagged Clone
Target Symbol Wls
Synonyms Gpr177
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>RN200060 representing NM_199408
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGGGGCAATTATAGAAAACATGAGCACCAAGAAGCTCTGCATTGTTGGAGGGATTCTTCTGGTTT
TCCAAATCGTTGCCTTTCTGGTGGGAGGCTTGATCGCTCCAGCACCCACAACGGCAGTGTCCTACGTGGC
AGCAAAATGTGTGGATGTCCGGAAGAACCACCATAAAACAAGATGGCTGATGCCCTGGGGACCAAACAAG
TGTAACAAGATCAATGACTTCGAAGAAGCAATTCCAAGGGAAATTGAAGCGAATGACATTGTGTTTTCTG
TACACATTCCCCTCCCTTCTATGGAGATGAGCCCATGGTTCCAGTTTATGCTGTTTATCCTGCAGATAGA
CATTGCTTTCAAGCTAAACAACCAAATCAGAGAAAATGCAGAAGTTTCCATGGATGTTTCCCTGGGTTAC
CGTGATGATATGTTTTCTGAGTGGACTGAAATGGCGCACGAAAGAGTACCACGTAAACTCAGATGCACTT
TCACATCCCCCAAGACCCCAGAGCATGAAGGTCGTCATTATGAATGTGATGTCCTTCCTTTCATGGAAAT
TGGGTCAGTGGCTCATAAGTATTACCTTCTAAATATCCGGCTACCTGTAAATGAGAAGAAGAAAATCAAT
GTTGGAATTGGGGAAATAAAGGACATTCGGTTGGTGGGAATCCACCAAAATGGAGGTTTCACTAAGGTAT
GGTTTGCTATGAAGACCTTCCTCACACCCAGCATCTTCATCATTATGGTGTGGTATTGGAGAAGGATCAC
CATGATGTCCCGACCTCCAGTGCTTCTGGAAAAAGTCATCTTTGCCCTTGGGATTTCCATGACCTTTATC
AATATCCCTGTGGAATGGTTTTCCATTGGATTTGATTGGACCTGGATGCTGTTATTTGGTGACATACGAC
AGGGCATCTTCTATGCAATGCTTCTTTCCTTCTGGATCATCTTCTGTGGCGAGCACATGATGGATCAACA
TGAGCGGAATCACATTGCAGGGTATTGGAAGCAAGTTGGACCAATTGCTGTTGGCTCTTTCTGCCTCTTC
ATATTTGACATGTGTGAGAGAGGAGTGCAACTCACAAATCCTTTCTACAGTATCTGGACTACAGATGTTG
GAACAGAACTGGCTATGGCTTTCATCATTGTGGCAGGTATCTGCCTCTGCCTCTACTTCCTGTTTCTGTG
TTTCATGGTATTTCAAGTATTCAGAAACATCAGTGGGAAACAGTCTAGCCTCCCAGCCATGAGCAAAGTC
CGGAGGCTGCACTATGAGGGTCTGATTTTCAGGTTCAAGTTCCTCATGCTGATCACCTTGGCTTGTGCTG
CCATGACTGTTATCTTCTTCATTGTTAGTCAGGTGACAGAAGGCCATTGGAAATGGGGTGGGGTCACAGT
TCAAGTGAGCAGTGCTTTCTTCACTGGAATCTATGGGATGTGGAACCTGTATGTCTTTGCTTTGATGTTC
TTGTATGCACCATCCCATAAGAACTATGGGGAAGACCAGTCTAATGGTGACCTGGGTGTCCACAGCGGGG
AAGAACTGCAGCTCACTACCACAATCACCCATGTAGATGGACCGACTGAGATCTACAAGTTGACCCGTAA
AGAAGCACAGGAGTAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_199408
Insert Size 1626 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_199408.2, NP_955440.1
RefSeq Size 2899 bp
RefSeq ORF 1626 bp
Locus ID 362065
UniProt ID Q6P689
Cytogenetics 2q45
Summary Regulates Wnt proteins sorting and secretion in a feedback regulatory mechanism. This reciprocal interaction plays a key role in the regulation of expression, subcellular location, binding and organelle-specific association of Wnt proteins. Plays also an important role in establishment of the anterior-posterior body axis formation during development (By similarity).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein.
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"NM_199408" in other vectors (3)
SKU Description Size Price
RR200060 Wls (Myc-DDK-tagged ORF) - Rat G protein-coupled receptor 177 (Gpr177), transcript variant 1, (10 ug) 10 ug
$755.00
RR200060L3 Lenti ORF clone of Wls (Myc-DDK-tagged ORF) - Rat G protein-coupled receptor 177 (Gpr177), transcript variant 1, (10 ug) 10 ug
$1,055.00
RR200060L4 Lenti ORF clone of Wls (mGFP-tagged ORF) - Rat G protein-coupled receptor 177 (Gpr177), transcript variant 1, (10 ug) 10 ug
$1,055.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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