GMPPB (NM_013334) Human Tagged ORF Clone

  • TrueORF®
SKU
RG219720
GMPPB (tGFP-tagged) - Human GDP-mannose pyrophosphorylase B (GMPPB), transcript variant 1
  $657.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol GMPPB
Synonyms LGMDR19; MDDGA14; MDDGB14; MDDGC14
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG219720 representing NM_013334
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGGCACTGATCTTAGTGGGGGGCTATGGGACGCGGCTACGGCCGCTGACGCTGAGCACCCCGAAGC
CACTGGTGGACTTCTGCAATAAGCCCATCTTGCTGCACCAAGTGGAGGCGCTAGCCGCGGCAGGCGTGGA
CCACGTGATCCTGGCCGTGAGCTACATGTCGCAGGTGCTGGAGAAGGAAATGAAGGCACAGGAGCAGAGG
CTGGGAATCCGAATCTCCATGTCCCATGAAGAGGAGCCTTTGGGGACAGCTGGGCCCCTGGCGCTGGCCC
GTGACCTACTCTCTGAGACTGCAGACCCTTTCTTCGTCCTCAACAGTGACGTGATCTGCGATTTCCCCTT
CCAAGCCATGGTGCAGTTCCACCGGCACCATGGCCAGGAGGGCTCCATCCTGGTGACCAAGGTGGAGGAA
CCCTCCAAGTACGGTGTGGTGGTGTGTGAGGCTGACACAGGCCGCATTCACCGGTTCGTGGAGAAGCCAC
AGGTGTTTGTGTCCAATAAGATCAACGCAGGCATGTACATCCTGAGCCCTGCAGTGCTGCGGCGCATCCA
GCTGCAGCCTACGTCCATTGAGAAGGAGGTCTTCCCCATTATGGCCAAGGAGGGGCAGCTATATGCCATG
GAGTTACAGGGCTTCTGGATGGACATTGGGCAGCCCAAGGACTTCCTCACTGGCATGTGCCTCTTCCTGC
AGTCACTGAGGCAGAAGCAGCCTGAGCGGCTGTGCTCAGGCCCTGGCATTGTGGGCAACGTGCTGGTGGA
CCCAAGTGCCCGCATCGGCCAGAACTGCAGCATTGGCCCCAATGTGAGCCTGGGACCTGGCGTGGTGGTC
GAAGATGGTGTGTGTATCCGGCGGTGCACGGTGCTGCGGGATGCCCGGATCCGTTCCCATTCCTGGCTTG
AGTCCTGCATTGTGGGCTGGCGCTGCCGCGTGGGTCAGTGGGTAAGCCTGTGGGCTGGGCTGGGTGGGGA
GAGGGGCGGGGAGTGTGCCTGCCTCCCTGACAAGGCCTATCCTCTCCTGGAGGTACGCATGGAGAACGTG
ACAGTGCTGGGTGAGGACGTCATAGTTAATGATGAGCTCTACCTCAACGGAGCCAGCGTGCTGCCCCACA
AGTCTATTGGCGAGTCAGTGCCAGAGCCTCGTATCATCATG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG219720 representing NM_013334
Red=Cloning site Green=Tags(s)

MKALILVGGYGTRLRPLTLSTPKPLVDFCNKPILLHQVEALAAAGVDHVILAVSYMSQVLEKEMKAQEQR
LGIRISMSHEEEPLGTAGPLALARDLLSETADPFFVLNSDVICDFPFQAMVQFHRHHGQEGSILVTKVEE
PSKYGVVVCEADTGRIHRFVEKPQVFVSNKINAGMYILSPAVLRRIQLQPTSIEKEVFPIMAKEGQLYAM
ELQGFWMDIGQPKDFLTGMCLFLQSLRQKQPERLCSGPGIVGNVLVDPSARIGQNCSIGPNVSLGPGVVV
EDGVCIRRCTVLRDARIRSHSWLESCIVGWRCRVGQWVSLWAGLGGERGGECACLPDKAYPLLEVRMENV
TVLGEDVIVNDELYLNGASVLPHKSIGESVPEPRIIM

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_013334
ORF Size 1161 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013334.3, NP_037466.2
RefSeq Size 1664 bp
RefSeq ORF 1164 bp
Locus ID 29925
UniProt ID Q9Y5P6
Cytogenetics 3p21.31
Domains hexapep, NTP_transferase
Protein Pathways Amino sugar and nucleotide sugar metabolism, Fructose and mannose metabolism, Metabolic pathways
Summary This gene is thought to encode a GDP-mannose pyrophosphorylase. The encoded protein catalyzes the conversion of mannose-1-phosphate and GTP to GDP-mannose, a reaction involved in the production of N-linked oligosaccharides. Alternatively spliced transcript variants encoding distinct isoforms have been described. provided by RefSeq, Jan 2009
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Documents
Resources
"NM_013334" in other vectors (4)
SKU Description Size Price
RC219720 GMPPB (Myc-DDK-tagged)-Human GDP-mannose pyrophosphorylase B (GMPPB), transcript variant 1 10 ug
$457.00
RC219720L3 Lenti ORF clone of Human GDP-mannose pyrophosphorylase B (GMPPB), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC219720L4 Lenti ORF clone of Human GDP-mannose pyrophosphorylase B (GMPPB), transcript variant 1, mGFP tagged 10 ug
$757.00
SC313251 GMPPB (untagged)-Human GDP-mannose pyrophosphorylase B (GMPPB), transcript variant 1 10 ug
$503.00

Plasmid Map for RG219720

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Cloning scheme for RG219720

Circular map for RG219720

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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