LCE2B (NM_014357) Human Tagged ORF Clone

  • TrueORF®
SKU
RG219058
LCE2B (tGFP-tagged) - Human late cornified envelope 2B (LCE2B)
  $350.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol LCE2B
Synonyms LEP10; SPRL1B; XP5
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG219058 representing NM_014357
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTTGCCAGCAAAACCAGCAGCAGTGCCAGCCCCCTCCCAAGTGTCCTCCCAAGTGTACCCCAAAAT
GTCCACCTAAGTGTCCCCCTAAATGCCTGCCCCAGTGCCCAGCTCCATGTTCCCCTGCAGTCTCTTCTTG
CTGTGGTCCCATCTCTGGGGGCTGCTGTGGTCCCAGCTCTGGGGGCTGCTGCAACTCTGGGGCTGGTGGC
TGCTGCCTGAGCCACCACAGGCCCCGTCTCTTCCACCGGCGCCGGCACCAGAGCCCCGACTGCTGTGAGA
GTGAACCTTCTGGGGGCTCTGGCTGCTGCCACAGCTCTGGGGGCTGCTGC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG219058 representing NM_014357
Red=Cloning site Green=Tags(s)

MSCQQNQQQCQPPPKCPPKCTPKCPPKCPPKCLPQCPAPCSPAVSSCCGPISGGCCGPSSGGCCNSGAGG
CCLSHHRPRLFHRRRHQSPDCCESEPSGGSGCCHSSGGCC

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_014357
ORF Size 330 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014357.5
RefSeq Size 619 bp
RefSeq ORF 333 bp
Locus ID 26239
UniProt ID O14633
Cytogenetics 1q21.3
Summary This gene is one of the at least 20 genes expressed during epidermal differentiation and located on chromosomal band 1q21. This gene is involved in epidermal differentiation, and it is expressed at high levels in normal and psoriatic skin, but not in cultured keratinocytes or in any other tested cell types or tissues. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_014357" in other vectors (4)
SKU Description Size Price
RC219058 LCE2B (Myc-DDK-tagged)-Human late cornified envelope 2B (LCE2B) 10 ug
$289.00
RC219058L3 Lenti ORF clone of Human late cornified envelope 2B (LCE2B), Myc-DDK-tagged 10 ug
$450.00
RC219058L4 Lenti ORF clone of Human late cornified envelope 2B (LCE2B), mGFP tagged 10 ug
$450.00
SC304086 LCE2B (untagged)-Human late cornified envelope 2B (LCE2B) 10 ug
$165.00

Plasmid Map for RG219058

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Cloning scheme for RG219058

Circular map for RG219058

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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