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KCNC1 (NM_004976) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG215716
KCNC1 (tGFP-tagged) - Human potassium voltage-gated channel, Shaw-related subfamily, member 1 (KCNC1), transcript variant B
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $913.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "KCNC1 or NM_004976"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol KCNC1
Synonyms EPM7; KV3.1; KV4; NGK2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_004976
ORF Size 1533 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004976.4
RefSeq Size 1602 bp
RefSeq ORF 1536 bp
Locus ID 3746
UniProt ID P48547
Cytogenetics 11p15.1
Protein Families Druggable Genome, Ion Channels: Potassium, Transmembrane
Summary This gene encodes a member of a family of integral membrane proteins that mediate the voltage-dependent potassium ion permeability of excitable membranes. Alternative splicing is thought to result in two transcript variants encoding isoforms that differ at their C-termini. These isoforms have had conflicting names in the literature: the longer isoform has been called both "b" and "alpha", while the shorter isoform has been called both "a" and "beta" (PMIDs 1432046, 12091563). provided by RefSeq, Oct 2014
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"NM_004976" in other vectors (6)
SKU Description Size Price
RC215716 KCNC1 (Myc-DDK-tagged)-Human potassium voltage-gated channel, Shaw-related subfamily, member 1 (KCNC1), transcript variant B 10 ug
$713.00
RC215716L1 Lenti ORF clone of Human potassium voltage-gated channel, Shaw-related subfamily, member 1 (KCNC1), transcript variant B, Myc-DDK-tagged 10 ug
$1,013.00
RC215716L2 Lenti ORF clone of Human potassium voltage-gated channel, Shaw-related subfamily, member 1 (KCNC1), transcript variant B, mGFP tagged 10 ug
$1,013.00
RC215716L3 Lenti ORF clone of Human potassium voltage-gated channel, Shaw-related subfamily, member 1 (KCNC1), transcript variant B, Myc-DDK-tagged 10 ug
$1,013.00
RC215716L4 Lenti ORF clone of Human potassium voltage-gated channel, Shaw-related subfamily, member 1 (KCNC1), transcript variant B, mGFP tagged 10 ug
$1,013.00
SC310304 KCNC1 (untagged)-Human potassium voltage-gated channel, Shaw-related subfamily, member 1 (KCNC1), transcript variant B 10 ug
$715.00

Plasmid Map for RG215716

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Cloning scheme for RG215716
Circular map for RG215716

file-alt Citations

Immunoproteomic studies on paediatric opsoclonus-myoclonus associated with neuroblastoma
Torres-Vega, Durán-Moreno, Sánchez Del Pino et al
J Neuroimmunol (2016) 297, 98-102 DOI: 10.1016/j.jneuroim.2016.05.015
Cited by 4
AI Summary: OriGene products contributed to this study by providing the anti-KCNC3 antibody (Cat# TA336524), which was used for immunoblotting to identify neuronal membrane proteins potentially implicated as antigens in paediatric opsoclonus-myoclonus associated with neuroblastoma.
A recurrent de novo mutation in KCNC1 causes progressive myoclonus epilepsy
Muona, Berkovic, Dibbens et al
Nat. Genet. (2014)
Cited by 317
AI Summary: OriGene’s pCMV-Entry human KCNC1 cDNA clone (encoding a C-terminal Myc-DDK tag) was used as the template for introducing the PME-associated KCNC1 mutation, enabling functional analysis of the mutant channel in this study.
Snippet: …We used the QuikChange kit (Stratagene) to engineer the missense mutation c.959G>A (p.Arg320His) into human KCNC1 cDNA (NM_004976;this construct corresponds to the KV3.1a isoform (511 amino acids), which has a shorter cytoplasmic C-terminal domain but identical biophysical properties in comparison to the longer KV3.1b (585 amino acids) isoform79) cloned in a pCMV-Entry vector obtained from OriGene Technologies. …
Immunoproteomic studies on paediatric opsoclonus-myoclonus associated with neuroblastoma
Torres-Vega, Durán-Moreno, del Pino
Journal of Neuroimmunology (2016) 297, 98
AI Summary: OriGene contributed GFP-tagged plasmids encoding human potassium-gated channel subunits Kv3.1, Kv3.2, Kv3.3, and Kv3.4 (catalog numbers RG215716, etc.), which were used to transfect HEK293T cells for antibody detection assays in the study.
Snippet: test for the presence of antibodies, HEK239T cells (American Type Culture Collection;CRL-3216) were transfected with plasmids containing human subunits of potassium-gated channels Kv3.1, Kv3.2, Kv3.3 and Kv3.4 (GFP-tagged clones from Origene: RG215716, ... Cite SaveSaving
Showing 1-3 of 3 papers.
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