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KRTAP5-9 (NM_005553) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG211005
KRTAP5 (tGFP-tagged) - Human keratin associated protein 5-9 (KRTAP5-9)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$489.00 $500.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol KRTAP5-9
Synonyms KRN1; KRTAP5-1; KRTAP5.9
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG211005 representing NM_005553
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCTGCTGTGGCTGCTCCGGAGGCTGTGGCTCCAGCTGTGGAGGCTGTGACTCCAGCTGTGGGAGCT
GTGGCTCTGGCTGCAGGGGCTGTGGCCCCAGCTGCTGTGCACCCGTCTACTGCTGCAAGCCCGTGTGCTG
CTGTGTTCCAGCCTGTTCCTGCTCTAGCTGTGGCAAGCGGGGCTGTGGCTCCTGTGGGGGCTCCAAGGGA
GGCTGTGGTTCTTGTGGCTGCTCCCAGTGCAGTTGCTGCAAGCCCTGCTGTTGCTCTTCAGGCTGTGGGT
CATCCTGCTGCCAGTGCAGCTGCTGCAAGCCCTACTGCTCCCAGTGCAGCTGCTGTAAGCCCTGTTGCTC
CTCCTCGGGTCGTGGGTCATCCTGCTGCCAATCCAGCTGCTGCAAGCCCTGCTGCTCATCCTCAGGCTGT
GGGTCATCCTGCTGCCAGTCCAGCTGCTGCAAGCCCTGCTGCTCCCAGTCCAGATGCTGTGTCCCTGTGT
GCTACCAGTGCAAGATC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG211005 representing NM_005553
Red=Cloning site Green=Tags(s)

MGCCGCSGGCGSSCGGCDSSCGSCGSGCRGCGPSCCAPVYCCKPVCCCVPACSCSSCGKRGCGSCGGSKG
GCGSCGCSQCSCCKPCCCSSGCGSSCCQCSCCKPYCSQCSCCKPCCSSSGRGSSCCQSSCCKPCCSSSGC
GSSCCQSSCCKPCCSQSRCCVPVCYQCKI

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_005553
ORF Size 507 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005553.4
RefSeq Size 1188 bp
RefSeq ORF 510 bp
Locus ID 3846
UniProt ID P26371
Cytogenetics 11q13.4
Summary In the hair cortex, hair keratin intermediate filaments are embedded in an interfilamentous matrix, consisting of hair keratin-associated protein (KRTAP), which are essential for the formation of a rigid and resistant hair shaft through their extensive disulfide bond cross-linking with abundant cysteine residues of hair keratins. The matrix proteins include the high-sulfur and high-glycine-tyrosine keratins.UniProtKB/Swiss-Prot Function
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"NM_005553" in other vectors (4)
SKU Description Size Price
RC211005 KRTAP5 (Myc-DDK-tagged)-Human keratin associated protein 5-9 (KRTAP5-9) 10 ug
$289.00 $300.00
RC211005L3 Lenti-ORF clone of KRTAP5 (Myc-DDK-tagged)-Human keratin associated protein 5-9 (KRTAP5-9) 10 ug
$600.00
RC211005L4 Lenti-ORF clone of KRTAP5 (mGFP-tagged)-Human keratin associated protein 5-9 (KRTAP5-9) 10 ug
$600.00
SC303665 KRTAP5 (untagged)-Human keratin associated protein 5-9 (KRTAP5-9) 10 ug
$330.00

Plasmid Map for RG211005

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG211005
Circular map for RG211005

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