EBLN2 (NM_018029) Human Tagged ORF Clone

  • TrueORF®
SKU
RG208579
EBLN2 (tGFP-tagged) - Human endogenous Borna-like N element-2 (EBLN2)
$489.00 $500.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol EBLN2
Synonyms EBLN-2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG208579 representing NM_018029
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGTTATTTTCTTAAATTGTATGCTTATGTTAATTCTCACAGTCTTTTTGTTTGGGTCTGTGACAGAT
CTTACAAAAGATCTTTTAGACCTATGATTCTTAACAAAATTAAAGAATTAAGTCGGAACCAATTTTCCAC
AATGTCTCATCTAAGAAAGGACTCACAGCCCAGCAGCCCAGGAGATGACGCAATGGACAGGAGTGGGCTC
CCTGACCTTCAAGGAAGATTTGAGCTATCTGGGAAAAACAGACAGTATCCACTGGATGCATTGGAACCCC
AACCCAGCATTGGGGATATTAAGGACATTAAAAAAGCAGCCAAGTCTATGCTAGACCCAGCACATAAATC
TCATTTCCACCCTGTGACCCCAAGTTTAGTATTCTTGTGTTTCATATTTGATGGGTTACACCAGGCATTA
CTGAGTGTTGGTGTGAGCAAGAGGTCTAATACTGTGGTTGGGAATGAGAACGAGGAAAGGGGTACTCCTT
ATGCTAGCAGATTCAAAGATATGCCTAACTTTATTGCCCTTGAGAAGTCATCAGTTCTCCGCCACTGCTG
TGACCTTTTGATAGGCATTGCGGCTGGATCAAGTGATAAGATTTGCACCAGCAGTCTCCAAGTTCAGAGA
CGATTCAAGGCAATGATGGCATCTATTGGAAGACTTTCACATGGTGAGAGTGCTGATCTGCTAATCAGCT
GCAATGCAGAATCAGCCATAGGTTGGATCAGCTCAAGACCGTGGGTTGGAGAATTAATGTTCACACTTCT
ATTTGGAGACTTTGAATCCCCTCTACACAAGCTACGCAAGTCAAGT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG208579 representing NM_018029
Red=Cloning site Green=Tags(s)

MGYFLKLYAYVNSHSLFVWVCDRSYKRSFRPMILNKIKELSRNQFSTMSHLRKDSQPSSPGDDAMDRSGL
PDLQGRFELSGKNRQYPLDALEPQPSIGDIKDIKKAAKSMLDPAHKSHFHPVTPSLVFLCFIFDGLHQAL
LSVGVSKRSNTVVGNENEERGTPYASRFKDMPNFIALEKSSVLRHCCDLLIGIAAGSSDKICTSSLQVQR
RFKAMMASIGRLSHGESADLLISCNAESAIGWISSRPWVGELMFTLLFGDFESPLHKLRKSS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_018029
ORF Size 816 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018029.3, NP_060499.3
RefSeq Size 1679 bp
RefSeq ORF 819 bp
Locus ID 55096
UniProt ID Q6P2I7
Cytogenetics 3p13
Summary May act as an RNA-binding protein. The C-terminal region is highly homologous to the bornavirus nucleocapsid N protein that binds viral RNA and oligomerizes. The viral protein also possesses a nuclear import and a nuclear export signal. These 2 signals seem absent in EBLN-2 supporting an unrelated function in Human.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_018029" in other vectors (6)
SKU Description Size Price
RC208579 EBLN2 (Myc-DDK-tagged)-Human endogenous Borna-like N element-2 (EBLN2) 10 ug
$300.00
RC208579L1 Lenti ORF clone of Human endogenous Borna-like N element-2 (EBLN2), Myc-DDK-tagged 10 ug
$600.00
RC208579L2 Lenti ORF clone of Human endogenous Borna-like N element-2 (EBLN2), mGFP tagged 10 ug
$600.00
RC208579L3 Lenti ORF clone of Human endogenous Borna-like N element-2 (EBLN2), Myc-DDK-tagged 10 ug
$600.00
RC208579L4 Lenti ORF clone of Human endogenous Borna-like N element-2 (EBLN2), mGFP tagged 10 ug
$600.00
SC304554 EBLN2 (untagged)-Human endogenous Borna-like N element-2 (EBLN2) 10 ug
$330.00

Plasmid Map for RG208579

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Cloning scheme for RG208579

Circular map for RG208579

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