SARS2 (NM_017827) Human Tagged ORF Clone

SKU RG208306

SARS2 (tGFP-tagged) - Human seryl-tRNA synthetase 2, mitochondrial (SARS2), nuclear gene encoding mitochondrial protein, transcript variant 2

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: SARS2
• Synonyms: mtSerRS; SARS; SARSM; SerRS; SerRSmt; SERS; SYS
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208306 representing NM_017827; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCTGCGTCCATGGCGCGGCGCTTGTGGCCTTTGCTGACTCGTCGGGGGTTCCGGCCCCGGGGAGGCT; GCATCTCCAACGATAGTCCAAGGAGAAGTTTCACTACAGAGAAACGAAACCGGAACCTCCTGTACGAGTA; TGCGCGCGAGGGCTACAGCGCACTCCCTCAGCTGGACATAGAGCGGTTCTGCGCATGCCCAGAAGAGGCC; GCACACGCCCTGGAGCTCCGCAAGGGGGAGCTGCGCTCGGCGGACCTGCCCGCGATCATCTCGACATGGC; AGGAGCTGAGGCAGCTGCAGGAGCAGATCCGGAGCCTGGAGGAAGAGAAGGCAGCTGTGACTGAGGCAGT; GCGGGCCCTGCTGGCAAACCAGGACAGTGGTGAAGTGCAGCAGGACCCCAAGTACCAGGGTCTGCGGGCA; CGTGGCCGGGAGATCCGGAAGGAGCTTGTTCACCTGTACCCCAGGGAGGCCCAGCTTGAGGAGCAGTTCT; ACCTGCAGGCGCTGAAGCTGCCCAACCAGACCCACCCAGACGTGCCCGTCGGGGATGAGAGCCAGGCTCG; AGTGCTCCACATGGTCGGAGACAAGCCAGTTTTCTCCTTCCAACCTCGGGGCCACCTGGAAATTGGCGAG; AAACTCGACATCATCCGTCAGAAGCGCCTGTCCCACGTGTCTGGCCACCGGTCCTATTACCTGCGCGGGG; CTGGAGCCCTCCTGCAGCACGGCCTGGTCAACTTCACATTCAACAAGCTTCTCCGCCGGGGCTTCACCCC; CATGACGGTGCCAGACCTTCTCCGCGGAGCAGTGTTTGAAGGCTGTGGGATGACACCAAATGCCAACCCA; TCCCAAATTTACAACATCGACCCTGCCCGCTTCAAAGATCTCAACCTGGCTGGAACAGCGGAGGTGGGGC; TTGCAGGCTACTTCATGGACCACACCGTGGCCTTCAGGGACCTGCCAGTCAGGATGGTTTGCTCCAGCAC; CTGCTACCGGGCAGAGACAAACACGGGACAGGAACCCCGGGGGCTGTATCGAGTACACCACTTCACCAAG; GTGGAGATGTTTGGGGTGACAGGCCCTGGGCTGGAGCAGAGCTCACAGCTGCTGGAGGAGTTCCTGTCCC; TTCAGATGGAGATCTTGACAGAGCTGGGCTTGCACTTCCGGGTCCTGGATATGCCCACCCAAGAACTGGG; CCTCCCCGCCTACCGCAAGTTTGACATTGAGGCCTGGATGCCAGGCCGAGGCCGCTTTGGAGAGGTCACC; AGTGCTTCCAACTGCACAGACTTCCAGAGCCGCCGCCTCCACATCATGTTCCAGACCGAGGCTGGGGAGC; TGCAGTTTGCCCATACGGTGAACGCCACCGCCTGTGCTGTCCCCCGCCTTCTCATCGCGCTCCTGGAGAG; TAACCAGCAGAAGGACGGCTCAGTGCTCGTGCCCCCTGCCCTCCAGTCCTACCTCGGCACTGATCGGATC; ACAGCCCCTACCCACGTGCCTCTCCAGTACATCGGCCCCAACCAGCCCCGGAAGCCTGGGCTGCCTGGCC; AGCCTGCTGTAAGC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208306 representing NM_017827; Red=Cloning site Green=Tags(s); ; MAASMARRLWPLLTRRGFRPRGGCISNDSPRRSFTTEKRNRNLLYEYAREGYSALPQLDIERFCACPEEA; AHALELRKGELRSADLPAIISTWQELRQLQEQIRSLEEEKAAVTEAVRALLANQDSGEVQQDPKYQGLRA; RGREIRKELVHLYPREAQLEEQFYLQALKLPNQTHPDVPVGDESQARVLHMVGDKPVFSFQPRGHLEIGE; KLDIIRQKRLSHVSGHRSYYLRGAGALLQHGLVNFTFNKLLRRGFTPMTVPDLLRGAVFEGCGMTPNANP; SQIYNIDPARFKDLNLAGTAEVGLAGYFMDHTVAFRDLPVRMVCSSTCYRAETNTGQEPRGLYRVHHFTK; VEMFGVTGPGLEQSSQLLEEFLSLQMEILTELGLHFRVLDMPTQELGLPAYRKFDIEAWMPGRGRFGEVT; SASNCTDFQSRRLHIMFQTEAGELQFAHTVNATACAVPRLLIALLESNQQKDGSVLVPPALQSYLGTDRI; TAPTHVPLQYIGPNQPRKPGLPGQPAVS; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017827
• ORF Size: 1554 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_017827.2, NP_060297.1
• RefSeq Size: 2067 bp
• RefSeq ORF: 1557 bp
• Locus ID: 54938
• UniProt ID: Q9NP81
• Cytogenetics: 19q13.2
• Domains: tRNA-synt_2b
• Protein Pathways: Aminoacyl-tRNA biosynthesis
• Summary: This gene encodes the mitochondrial seryl-tRNA synthethase precursor, a member of the class II tRNA synthetase family. The mature enzyme catalyzes the ligation of Serine to tRNA(Ser) and participates in the biosynthesis of selenocysteinyl-tRNA(sec) in mitochondria. The enzyme contains an N-terminal tRNA binding domain and a core catalytic domain. It functions in a homodimeric form, which is stabilized by tRNA binding. This gene is regulated by a bidirectional promoter that also controls the expression of mitochondrial ribosomal protein S12. Both genes are within the critical interval for the autosomal dominant deafness locus DFNA4 and might be linked to this disease. Multiple transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Mar 2009]