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MCAF2 (ATF7IP2) (NM_024997) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG206076
ATF7IP2 (tGFP-tagged) - Human activating transcription factor 7 interacting protein 2 (ATF7IP2)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $825.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MCAF2
Synonyms MCAF2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG206076 representing NM_024997
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAAGTCCAGATAGAAGTAAACGGAAGATATTAAAAGCCAAAAAGACAATGCCCCTAAGTTGCCGGA
AGCAAGTAGAGATGCTGAATAAGTCAAGGAATGTTGAAGCGCTGAAAACAGCAATTGGGAGTAATGTTCC
AAGCGGTAATCAGAGTTTCAGTCCTAGTGTCATAACTAGGACGACTGAAATAACCAAATGTAGCCCTTCT
GAAAATGGTGCATCCTCATTGGACTCTAATAAAAATTCAATATCAGAGAAAAGTAAAGTATTCTCTCAGA
ATTGCATAAAACCAGTAGAAGAAATTGTTCATTCAGAAACAAAATTGGAACAAGTTGTTTGTTCGTACCA
AAAGCCAAGTAGAACAACAGAATCCCCCAGCAGAGTCTTCACAGAAGAGGCAAAAGATTCACTGAACACT
TCTGAAAACGATTCTGAGCATCAGACAAATGTAACAAGATCCCTTTTTGAGCATGAGGGGGCTTGTAGTC
TAAAGTCCAGTTGCTGTCCACCCAGTGTATTGAGTGGTGTTGTTCAGATGCCAGAGTCTACAGTAACCAG
TACCGTGGGTGACAAGAAAACTGACCAGATGGTTTTCCATTTAGAAACAAACTCCAATTCAGAATCACAT
GATAAAAGGCAAAGTGACAACATTTTATGTTCAGAAGACTCAGGTTTTGTGCCTGTTGAGAAAACACCTA
ATTTGGTGAATTCAGTCACTTCTAACAACTGTGCTGATGACATTTTGAAAACTGATGAGTGTAGTAGAAC
CAGTATTTCAAATTGTGAAAGTGCAGACTCAACATGGCAGTCATCACTTGACACTAATAACAACAGCCAT
TATCAAAAGAAGAGGATGTTTTCAGAAAACGAGGAAAATGTTAAACGCATGAAAACTTCAGAGCAAATTA
ATGAAAATATTTGTGTAAGTTTGGAAAGGCAAACAGCATTCCTGGAACAGGTCAGACATTTGATTCAGCA
GGAGATCTATAGCATAAATTATGAACTATTTGATAAGAAACTGAAAGAATTGAACCAACGCATTGGGAAG
ACAGAGTGCAGAAATAAGCATGAAGGAATAGCTGATAAACTTTTGGCAAAAATAGCAAAACTTCAAAGAC
GTATTAAAACAGTATTATTATTTCAAAGGAATTGTTTGAAACCAAACATGTTATCCAGTAATGGAGCCTC
TAAGGTTGCAAATTCAGAGGCTATGATTTTGGATAAGAATCTTGAGTCAGTTAATAGTCCAATTGAAAAG
TCTTCTGTGAATTATGAGCCTTCTAACCCTTCCGAAAAAGGAAGTAAAAAAATTAATTTGTCATCAGATC
AAAATAAGTCTGTTTCTGAAAGTAACAATGATGATGTTATGTTGATTTCTGTGGAAAGTCCTAATTTGAC
AACTCCAATTACATCAAATCCAACAGATACCAGAAAAATTACATCAGGAAATTCTAGCAATTCTCCCAAT
GCTGAAGTTATGGCTGTACAGAAGAAACTTGATTCTATAATTGATTTGACAAAAGAAGGCCTATCCAACT
GCAATACAGAAAGTCCAGTATCCCCCCTGGAGTCACATTCGAAAGCTGCTTCAAACTCAAAGGAAACAAC
CCCATTGGCACAAAATGCAGTCCAGGTTCCTGAGTCCTTTGAGCACCTGCCACCTCTCCCAGAACCACCA
GCACCACTACCTGAATTAGTAGACAAAACCCGAGACACACTTCCTCCCCAGAAGCCTGAGCTCAAAGTGA
AACGGGTTTTCAGACCCAATGGCATTGCCCTGACTTGGAATATAACCAAAATCAATCCCAAGTGTGCTCC
TGTAGAAAGCTACCACCTCTTCCTGTGTCATGAGAACTCTAATAATAAGTTGATTTGGAAGAAGATTGGA
GAAATTAAAGCTTTACCACTCCCCATGGCCTGTACTTTATCTCAGTTTTTAGCTTCCAACAGATACTATT
TTACTGTCCAATCAAAAGATATTTTTGGACGATATGGACCATTCTGTGATATAAAATCTATCCCTGGGTT
TTCTGAAAATCTTACG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG206076 representing NM_024997
Red=Cloning site Green=Tags(s)

MASPDRSKRKILKAKKTMPLSCRKQVEMLNKSRNVEALKTAIGSNVPSGNQSFSPSVITRTTEITKCSPS
ENGASSLDSNKNSISEKSKVFSQNCIKPVEEIVHSETKLEQVVCSYQKPSRTTESPSRVFTEEAKDSLNT
SENDSEHQTNVTRSLFEHEGACSLKSSCCPPSVLSGVVQMPESTVTSTVGDKKTDQMVFHLETNSNSESH
DKRQSDNILCSEDSGFVPVEKTPNLVNSVTSNNCADDILKTDECSRTSISNCESADSTWQSSLDTNNNSH
YQKKRMFSENEENVKRMKTSEQINENICVSLERQTAFLEQVRHLIQQEIYSINYELFDKKLKELNQRIGK
TECRNKHEGIADKLLAKIAKLQRRIKTVLLFQRNCLKPNMLSSNGASKVANSEAMILDKNLESVNSPIEK
SSVNYEPSNPSEKGSKKINLSSDQNKSVSESNNDDVMLISVESPNLTTPITSNPTDTRKITSGNSSNSPN
AEVMAVQKKLDSIIDLTKEGLSNCNTESPVSPLESHSKAASNSKETTPLAQNAVQVPESFEHLPPLPEPP
APLPELVDKTRDTLPPQKPELKVKRVFRPNGIALTWNITKINPKCAPVESYHLFLCHENSNNKLIWKKIG
EIKALPLPMACTLSQFLASNRYYFTVQSKDIFGRYGPFCDIKSIPGFSENLT

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_024997
ORF Size 2046 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024997.5
RefSeq Size 3677 bp
RefSeq ORF 2049 bp
Locus ID 80063
UniProt ID Q5U623
Cytogenetics 16p13.2-p13.13
Summary Recruiter that couples transcriptional factors to general transcription apparatus and thereby modulates transcription regulation and chromatin formation. Can both act as an activator or a repressor depending on the context. Mediates MBD1-dependent transcriptional repression, probably by recruiting complexes containing SETDB1. The complex formed with MBD1 and SETDB1 represses transcription and probably couples DNA methylation and histone H3 'Lys-9' trimethylation (H3K9me3) activity (Probable).UniProtKB/Swiss-Prot Function
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"NM_024997" in other vectors (4)
SKU Description Size Price
RC206076 ATF7IP2 (Myc-DDK-tagged)-Human activating transcription factor 7 interacting protein 2 (ATF7IP2) 10 ug
$589.00 $625.00
RC206076L3 Lenti-ORF clone of ATF7IP2 (Myc-DDK-tagged)-Human activating transcription factor 7 interacting protein 2 (ATF7IP2) 10 ug
$925.00
RC206076L4 Lenti-ORF clone of ATF7IP2 (mGFP-tagged)-Human activating transcription factor 7 interacting protein 2 (ATF7IP2) 10 ug
$925.00
SC305209 ATF7IP2 (untagged)-Human activating transcription factor 7 interacting protein 2 (ATF7IP2) 10 ug
$688.00

Plasmid Map for RG206076

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG206076
Circular map for RG206076

file-alt Citations

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