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CEP70 (NM_024491) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG205474
CEP70 (tGFP-tagged) - Human centrosomal protein 70kDa (CEP70)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $756.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CEP70
Synonyms BITE
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG205474 representing NM_024491
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTTCCGGTAGCCCCTAAACCCCAGGATTCCAGTCAACCATCAGACAGACTCATGACTGAAAAACAGC
AGGAAGAAGCAGAATGGGAAAGCATAAATGTGCTATTGATGATGCATGGCTTAAAACCTTTGTCTCTAGT
CAAAAGAACAGATCTCAAAGATCTCATCATTTTTGACAAACAGTCATCACAAAGGATGAGACAGAATTTG
AAATTGTTGGTGGAAGAAACATCATGTCAACAGAACATGATACAGGAGCTTATAGAAACTAATCAACAGC
TTAGAAATGAACTTCAGCTAGAGCAAAGCCGAGCAGCCAATCAAGAACAACGAGCTAATGACTTGGAACA
AATTATGGAAAGTGTGAAATCCAAAATTGGTGAATTGGAGGATGAATCACTAAATAGGGCTTGCCACCAA
CAGAATAAAATAAAAGATCTTCAAAAGGAGCAGAAAACTTTGCAGGTGAAGTGCCAGCATTATAAGAAAA
AACGAACGGAGCAAGAAGAAACTATTGCTTCTTTGCAAATGGAAGTCTGTAGATTAAAAAAGGAGGAAGA
AGATCGCATTGTCACTCAAAACAGAGTGTTTGCCTATCTGTGCAAAAGAGTTCCTCATACCGTCTTGGAT
AGACAGTTGCTTTGTCTAATTGATTACTATGAATCTAAAATTAGAAAAATTCATACACAAAGGCAATATA
AAGAAGATGAAAGTCAGTCAGAAGAAGAAAACGACTACAGAAATCTGGATGCCTCACCAACTTATAAAGG
CCTTTTAATGTCATTACAGAATCAACTCAAGGAATCAAAATCTAAGATTGATGCACTTTCAAGTGAAAAG
CTGAACCTCCAAAAAGATTTGGAAACCAGGCCTACGCAGCATGAATTAAGACTTTATAAACAGCAGGTGA
AGAAGCTGGAAAAAGCCCTTAAGAAAAACGTCAAATTACAGGAGCTTATTAATCATAAGAAGGCTGAGGA
CACAGAGAAGAAAGATGAGCCCAGCAAATATAATCAGCAACAGGCCCTAATTGACCAGAGATACTTTCAG
GTGCTGTGTAGCATCAATTCAATTATCCACAATCCAAGAGCTCCAGTAATAATTTATAAACAGACCAAAG
GGGGAGTCCAAAATTTTAATAAAGATCTTGTTCAAGATTGTGGATTTGAGCATCTTGTTCCTGTAATAGA
AATGTGGGCAGATCAACTGACATCCTTAAAGGATTTGTATAAGTCCTTGAAAACACTATCTGCAGAACTG
GTACCTTGGCTTAATTTGAAGAAGCAGGATGAAAATGAAGGTATCAAAGTTGAAGATTTGTTGTTTATAG
TAGATACTATGCTGGAAGAAGTTGAAAATAAGGAAAAGGACAGCAATATGCCACACTTTCAAACTTTGCA
AGCTATTGTTTCTCACTTCCAAAAGTTATTTGATGTGCCTTCTTTAAATGGAGTCTATCCCCGAATGAAT
GAAGTTTATACTAGGCTTGGAGAAATGAACAATGCTGTGAGAAACCTCCAAGAACTCTTAGAATTAGATA
GTTCATCCTCATTGTGTGTGCTAGTAAGCACTGTTGGAAAACTCTGTAGGCTGATTAATGAAGATGTGAA
TGAGCAGGTTATGCAGGTATTAGGACCTGAAGACCTCCAGAGCATTATCTACAAATTGGAAGAACACGAG
GAATTTTTCCCAGCATTTCAGGCATTTACTAATGATCTACTTGAAATCTTAGAAATTGATGACTTGGATG
CCATTGTACCTGCAGTAAAGAAATTAAAAGTACTTTCATAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG205474 representing NM_024491
Red=Cloning site Green=Tags(s)

MFPVAPKPQDSSQPSDRLMTEKQQEEAEWESINVLLMMHGLKPLSLVKRTDLKDLIIFDKQSSQRMRQNL
KLLVEETSCQQNMIQELIETNQQLRNELQLEQSRAANQEQRANDLEQIMESVKSKIGELEDESLNRACHQ
QNKIKDLQKEQKTLQVKCQHYKKKRTEQEETIASLQMEVCRLKKEEEDRIVTQNRVFAYLCKRVPHTVLD
RQLLCLIDYYESKIRKIHTQRQYKEDESQSEEENDYRNLDASPTYKGLLMSLQNQLKESKSKIDALSSEK
LNLQKDLETRPTQHELRLYKQQVKKLEKALKKNVKLQELINHKKAEDTEKKDEPSKYNQQQALIDQRYFQ
VLCSINSIIHNPRAPVIIYKQTKGGVQNFNKDLVQDCGFEHLVPVIEMWADQLTSLKDLYKSLKTLSAEL
VPWLNLKKQDENEGIKVEDLLFIVDTMLEEVENKEKDSNMPHFQTLQAIVSHFQKLFDVPSLNGVYPRMN
EVYTRLGEMNNAVRNLQELLELDSSSSLCVLVSTVGKLCRLINEDVNEQVMQVLGPEDLQSIIYKLEEHE
EFFPAFQAFTNDLLEILEIDDLDAIVPAVKKLKVLSY

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_024491
ORF Size 1791 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024491.2, NP_077817.2
RefSeq Size 2678 bp
RefSeq ORF 1794 bp
Locus ID 80321
UniProt ID Q8NHQ1
Cytogenetics 3q22.3
Summary Plays a role in the organization of both preexisting and nascent microtubules in interphase cells. During mitosis, required for the organization and orientation of the mitotic spindle.UniProtKB/Swiss-Prot Function
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Resources
"NM_024491" in other vectors (4)
SKU Description Size Price
RC205474 CEP70 (Myc-DDK-tagged)-Human centrosomal protein 70kDa (CEP70) 10 ug
$289.00 $556.00
RC205474L3 Lenti-ORF clone of CEP70 (Myc-DDK-tagged)-Human centrosomal protein 70kDa (CEP70) 10 ug
$856.00
RC205474L4 Lenti-ORF clone of CEP70 (mGFP-tagged)-Human centrosomal protein 70kDa (CEP70) 10 ug
$856.00
SC112234 CEP70 (untagged)-Human centrosomal protein 70kDa (CEP70) 10 ug
$557.00

Plasmid Map for RG205474

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG205474
Circular map for RG205474

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