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CD19 (NM_001178098) Human Tagged Lenti ORF Clone

  • LentiORF®
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SKU
RC230267L2
Lenti-ORF clone of CD19 (mGFP-tagged)-Human CD19 molecule (CD19), transcript variant 1
ORF Plasmid:
mGFP DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $1,078.00
5 Days*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CD19
Synonyms B4; CVID3
Vector pLenti-C-mGFP
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection None
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC230267).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001178098
ORF Size 1671 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001178098.1
RefSeq Size 1968 bp
RefSeq ORF 1674 bp
Locus ID 930
UniProt ID P15391
Cytogenetics 16p11.2
Protein Families Druggable Genome, Transmembrane
Protein Pathways B cell receptor signaling pathway, Hematopoietic cell lineage, Primary immunodeficiency
MW 61.6 kDa
Summary This gene encodes a member of the immunoglobulin gene superfamily. Expression of this cell surface protein is restricted to B cell lymphocytes. This protein is a reliable marker for pre-B cells but its expression diminishes during terminal B cell differentiation in antibody secreting plasma cells. The protein has two N-terminal extracellular Ig-like domains separated by a non-Ig-like domain, a hydrophobic transmembrane domain, and a large C-terminal cytoplasmic domain. This protein forms a complex with several membrane proteins including complement receptor type 2 (CD21) and tetraspanin (CD81) and this complex reduces the threshold for antigen-initiated B cell activation. Activation of this B-cell antigen receptor complex activates the phosphatidylinositol 3-kinase signalling pathway and the subsequent release of intracellular stores of calcium ions. This protein is a target of chimeric antigen receptor (CAR) T-cells used in the treatment of lymphoblastic leukemia. Mutations in this gene are associated with the disease common variable immunodeficiency 3 (CVID3) which results in a failure of B-cell differentiation and impaired secretion of immunoglobulins. CVID3 is characterized by hypogammaglobulinemia, an inability to mount an antibody response to antigen, and recurrent bacterial infections. Alternative splicing results in multiple transcript variants encoding distinct isoforms. provided by RefSeq, Jul 2020
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Documents
Resources
"NM_001178098" in other vectors (6)
SKU Description Size Price
RC230267 CD19 (Myc-DDK-tagged)-Human CD19 molecule (CD19), transcript variant 1 10 ug
$289.00 $778.00
RC230267L1 Lenti-ORF clone of CD19 (Myc-DDK-tagged)-Human CD19 molecule (CD19), transcript variant 1 10 ug
$1,078.00
RC230267L3 Lenti-ORF clone of CD19 (Myc-DDK-tagged)-Human CD19 molecule (CD19), transcript variant 1 10 ug
$1,078.00
RC230267L4 Lenti-ORF clone of CD19 (mGFP-tagged)-Human CD19 molecule (CD19), transcript variant 1 10 ug
$1,078.00
RG230267 CD19 (tGFP-tagged) - Human CD19 molecule (CD19), transcript variant 1 10 ug
$489.00 $978.00
SC328905 CD19 (untagged)-Human CD19 molecule (CD19) transcript variant 1 10 ug
$779.00

Plasmid Map for RC230267L2

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Cloning scheme for RC230267L2
Circular map for RC230267L2

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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