HSPC302 (TBCK) (NM_001163435) Human Tagged Lenti ORF Clone

  • LentiORF®
SKU
RC228610L4
Lenti ORF clone of Human TBC1 domain containing kinase (TBCK), transcript variant 1, mGFP tagged
  $1,118.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol HSPC302
Synonyms HSPC302; IHPRF3; TBCKL
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC228610).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001163435
ORF Size 2679 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001163435.1, NP_001156907.1
RefSeq ORF 2682 bp
Locus ID 93627
UniProt ID Q8TEA7
Cytogenetics 4q24
Protein Families Druggable Genome, Protein Kinase
MW 100.5 kDa
Summary This gene encodes a protein that contains a protein kinase domain, a Rhodanase-like domain and the Tre-2/Bub2/Cdc16 (TBC) domain. The encoded protein is thought to play a role in actin organization, cell growth and cell proliferation by regulating the mammalian target of the rapamycin (mTOR) signaling pathway. This protein may also be involved in the transcriptional regulation of the components of the mTOR complex. Alternative splicing results in multiple transcript variants. provided by RefSeq, Mar 2014
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Documents
Resources
"NM_001163435" in other vectors (4)
SKU Description Size Price
RC228610 TBCK (Myc-DDK-tagged)-Human TBC1 domain containing kinase (TBCK), transcript variant 1 10 ug
$818.00
RC228610L3 Lenti ORF clone of Human TBC1 domain containing kinase (TBCK), transcript variant 1, Myc-DDK-tagged 10 ug
$1,118.00
RG228610 TBCK (tGFP-tagged) - Human TBC1 domain containing kinase (TBCK), transcript variant 1 10 ug
$1,018.00
SC327245 TBCK (untagged)-Human TBC1 domain containing kinase (TBCK) transcript variant 1 10 ug
$900.00

Plasmid Map for RC228610L4

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Cloning scheme for RC228610L4

Circular map for RC228610L4

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